Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3

Prator, Cecilia A.; Kashiwagi, Chloe M.; Vončina, Darko; Almeida, Rodrigo P. P.

doi:10.1016/j.virol.2017.07.003

Cited by 16 publications

(18 citation statements)

References 31 publications

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“…Previously it was assumed that if mealybugs are not on grapevines, they can neither acquire the virus nor transmit it. However, a recent study shows GLRaV-3 infecting a non-Vitis plant, Nicotiana benthamiana Domin (Solanales: Solanaceae) (Prator et al 2017). Therefore, further testing is required to confirm whether or not GLRaV-3 can be retained on, or acquired from clover lines.…”

Section: Discussionmentioning

confidence: 99%

Mealybug preference among clover cultivars: testing potential groundcover plants to dissociate mealybugs from grapevines

Sandanayaka

Davis

Jesson

2018

NZPP

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The choice of groundcover plants used under grapevines may reduce the quantity of mealybugs found on grapevines in New Zealand vineyards. Preferences of Pseudococcus calceolariae and P. longispinus mealybugs on five clover cultivars were tested under ‘no-choice’ and ‘choice’ conditions. Two plants of each cultivar: ‘Karridale’(KS) (Trifolium subterraneum, L.), ‘Tripoli’ (TW), ‘Nomad’ (N) (T. repens), ‘Crimson cv’ (C) (T. incarnatum) and ‘Strawberry cv’ (S) (T. fragiferum) grown in pots, were arranged in randomised designs for the no-choice and choice tests and inoculated with newly emerged mealybugs. In the no-choice test, significantly more mealybugs (either species) were observed on KS and C than on TW, N, or S plants after 21 days. In the choice test, significantly more P. calceolariae were observed on KS than on TW, N, or S, while mealybug numbers on C were intermediate. Numbers of P. longispinus were significantly higher on KS and C than on TW, N or S. Mealybugs demonstrated preferences for KS and C over TW, N or S. These findings could aid development of recommendations for groundcover management in vineyards.

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Section: Discussionmentioning

confidence: 99%

Mealybug preference among clover cultivars: testing potential groundcover plants to dissociate mealybugs from grapevines

Sandanayaka

Davis

Jesson

2018

NZPP

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“…First instars were used for all experiments because they were shown to be the most efficient vector of GLRaV-3 [ 9 ]. GLRaV-3 virions (accession LR101; variant I) were purified as previously described [ 15 , 16 ]. Whole virion integrity from purifications was confirmed previously [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…GLRaV-3 virions (accession LR101; variant I) were purified as previously described [ 15 , 16 ]. Whole virion integrity from purifications was confirmed previously [ 15 ]. Three resuspended virion purification aliquots from either N. benthamiana expressing the turnip mosaic virus P1/HC-Pro or V. vinifera were immediately pooled and added to 1.8 mL of an artificial diet composed of 15% sucrose and 1% BSA in TE, as described previously [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…Three resuspended virion purification aliquots from either N. benthamiana expressing the turnip mosaic virus P1/HC-Pro or V. vinifera were immediately pooled and added to 1.8 mL of an artificial diet composed of 15% sucrose and 1% BSA in TE, as described previously [ 6 ]. Transgenic tobacco expressing P1/HC-Pro, an antiviral silencing suppressor, were used because transmission efficiencies using wildtype tobacco were very low, and previously published studies showed increased numbers of P1/HC-Pro N. benthamiana plants became infected when compared to wildtype N. benthamiana [ 15 ]. An artificial diet without the addition of purified virions was used as a control.…”

Section: Methodsmentioning

confidence: 99%

“…During feeding, mealybugs extend the stylets into plant vascular tissues where the ingestion of primarily phloem begins, although xylem-sap feeding has also been documented through electropenetrography [ 14 ]. GLRaV-3 is phloem-restricted in both Vitis vinifera and Nicotiana benthamiana; thus, mealybug vectors must ingest phloem-sap to acquire virions for subsequent transmission [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus

Prator

Almeida

2020

Viruses

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Grapevine leafroll disease is one of the most important virus diseases of grapevines and occurs in every major grape-growing region of the world. The vector-transmission mechanisms of the causative agent, Grapevine leafroll-associated virus 3 (GLRaV-3), remain poorly understood. We show that the vine mealybug, Planococcus ficus, feeds through a membrane feeding system on GLRaV-3 viral purifications from both V. vinifera and N. benthamiana and transmits the virus to test plants from plants from both species. Building on this strategy, we used an immunofluorescence approach to localize virions to two retention sites in P. ficus mouthparts. Assays testing molecules capable of blocking virus transmission demonstrated that GLRaV-3-transmission by P. ficus could be disrupted. Our results indicate that our membrane feeding system and transmission-blocking assays are a valid approach and can be used to screen other candidate blocking molecules.

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RETRACTED ARTICLE: Albinism and mosaicism in Apocynum venetum associated with viral infections in China

et al. 2018

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Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3

Cited by 16 publications

References 31 publications

Mealybug preference among clover cultivars: testing potential groundcover plants to dissociate mealybugs from grapevines

Mealybug preference among clover cultivars: testing potential groundcover plants to dissociate mealybugs from grapevines

A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus

RETRACTED ARTICLE: Albinism and mosaicism in Apocynum venetum associated with viral infections in China

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