Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva

Brown, C.G.D.; Stagg, D.A.; Purnell, R.E.; Kanhai, G. K.; Payne, Rose

doi:10.1038/245101a0

Cited by 181 publications

(80 citation statements)

References 2 publications

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“…CD8 T-cell responses were studied using blood leukocytes collected 15 days after the final challenge. Cell lines transformed by infection with Theileria annulata were established from each animal at the outset of the study by in vitro infection of peripheral blood mononuclear cells (PBMC) with the sporozoite stage of the parasite, as described previously [51].…”

Section: Animals and Immunizationmentioning

confidence: 99%

Identification of immediate early gene products of bovine herpes virus 1 (BHV-1) as dominant antigens recognized by CD8 T cells in immune cattle

Hart

MacHugh

Sheldrake

et al. 2017

Journal of General Virology

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In common with other herpes viruses, bovine herpes virus 1 (BHV-1) induces strong virus-specific CD8 T-cell responses. However, there is a paucity of information on the antigenic specificity of the responding T-cells. The development of a system to generate virus-specific CD8 T-cell lines from BHV-1-immune cattle, employing Theileria-transformed cell lines for antigen presentation, has enabled us to address this issue. Use of this system allowed the study to screen for CD8 T-cell antigens that are efficiently presented on the surface of virus-infected cells. Screening of a panel of 16 candidate viral gene products with CD8 T-cell lines from 3 BHV-1-immune cattle of defined MHC genotypes identified 4 antigens, including 3 immediate early (IE) gene products (ICP4, ICP22 and Circ) and a tegument protein (UL49). Identification of the MHC restriction specificities revealed that the antigens were presented by two or three class I MHC alleles in each animal. Six CD8 T-cell epitopes were identified in the three IE proteins by screening of synthetic peptides. Use of an algorithm (NetMHCpan) that predicts the peptide-binding characteristics of restricting MHC alleles confirmed and, in some cases refined, the identity of the epitopes. Analyses of the epitope specificity of the CD8 T-cell lines showed that a large component of the response is directed against these IE epitopes. The results indicate that these IE gene products are dominant targets of the CD8 T-cell response in BHV-I-immune cattle and hence are prime-candidate antigens for the generation of a subunit vaccine.

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Section: Animals and Immunizationmentioning

confidence: 99%

Identification of immediate early gene products of bovine herpes virus 1 (BHV-1) as dominant antigens recognized by CD8 T cells in immune cattle

Hart

MacHugh

Sheldrake

et al. 2017

Journal of General Virology

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“…Among several Theileria species, T. parva is highly pathogenic for cattle and cause fatal lymphoproliferative diseases known as East Coast fever (Brown et al, 1973;Irvin et al, 1975;Lawrence and 1987). The MDM2 protein acts as a negative regulator of p53 through two known mechanisms: First, MDM2 binds p53 through its NH2-terminus, resulting in inhibition of its transcriptional activity (Momand et al, 1992), and second, the COOH-terminus of MDM2 serves as an E3 ubiquitin ligase targeting p53 for proteasomal degradation (Haupt et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

MDM2 regulates a novel form of incomplete neoplastic transformation of Theileria parva infected lymphocytes

Hayashida

Kajino

Hattori

et al. 2013

Experimental and Molecular Pathology

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“…T. parva is a protozoal pathogen of cattle that replicates in the cytoplasm of lymphocytes (7), inducing lymphoblastoid transformation resulting in clonal expansion of the infected cell (8,9). These transformed cells, primarily T cells, disseminate to lymphoid and nonlymphoid tissues, including the lung, kidney, and, intestine, where they mimic the behavior of lymphoid tumors (10,11).…”

Section: Introductionmentioning

confidence: 99%

Parasite-mediated nuclear factor κB regulation in lymphoproliferation caused byTheileria parva infection

Palmer

Machado²,

Fernández³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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one of the authors regrets that she inadvertently omitted references to the computer program and protein potential function that the authors used for their simulations of barnase cited above. The following sentence should have been the first sentence of the Methods section: Molecular dynamics simulations were performed with the program ENCAD (44) and the potential energy function of Levitt et al. (45).

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Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva

Cited by 181 publications

References 2 publications

Identification of immediate early gene products of bovine herpes virus 1 (BHV-1) as dominant antigens recognized by CD8 T cells in immune cattle

Identification of immediate early gene products of bovine herpes virus 1 (BHV-1) as dominant antigens recognized by CD8 T cells in immune cattle

MDM2 regulates a novel form of incomplete neoplastic transformation of Theileria parva infected lymphocytes

Parasite-mediated nuclear factor κB regulation in lymphoproliferation caused byTheileria parva infection

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