Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model

Abstract

BackgroundIn the present study, the influence of bacterial infection, lipopolysacharides (LPS) and hydroxyethyl starch (HES) on platelet function in a parallel plate flow chamber were measured. Experiments were performed with non-activated and protease-activating-receptor (PAR) 4 agonist activated platelets. Comparative measurements were in vivo capillary bleeding time, platelet function analyzer and impedance aggregometry.ResultsPAR 4 agonist did not increase platelet adhesion of platelets from dogs with bacterial inflammation in the flow chamber in contrast to platelets of healthy dogs. Except from impedance aggregometry with lower sensitivity and specificity, PFA did not detect platelet dysfunctions in dogs with infection. In vitro addition of LPS or HES significantly reduced platelet covered area after PAR-activation.ConclusionsThe flow chamber detects platelet dysfunctions in dogs with inflammatory diseases. In vitro addition of LPS highlights the inhibiting effect of bacterial wall components on platelet function. Platelet dysfunction induced by infection could possibly also be diagnosed after treatment of sepsis with colloids has commenced. The flow chamber could be a useful tool to detect sepsis associated platelet dysfunction given that larger prospective trials confirm these findings from a proof of concept study.

Platelets and Infections – Complex Interactions with Bacteria

“…This latter result suggests that beyond their role in IE due to S. aureus , platelets participate in the pathophysiology of S. aureus -induced sepsis (127). …”

The curious case of how mimicking physiological complexity in in vitro models of the human respiratory system influences the inflammatory responses. A preliminary study focused on gold nanoparticles

“…Further controls were used to test the robustness of the chosen experimental set-up, such as the use of uncoated channels. The experiments under dynamic conditions were performed based on slightly adapted protocols publicly available from Cellix Ltd and that have been used in a number of studies (Choi et al, 2008;Dominical et al, 2015;Ferkau et al, 2013;Long et al, 2004;Munir et al, 2015;Nissinen et al, 2010;Robinson, Kashanin, O'Dowd, Williams, & Walsh, 2008;Wu, Mitchell, & Walsh, 2005). Briefly, after washing the channel with 30 μL DMEM media, THP-1 cells were introduced at shear stress of 0.03 dyne/cm 2 .…”

Abnormal platelet activity in dogs and cats – impact and measurement

“…, Ferkau et al . ). Thrombocytopenia will not be covered further in this article as it is well described elsewhere (Johnstone , O'Marra et al .…”

“…). The Multiplate has also been used to detect reduced platelet aggregation induced in vitro by the addition of lipopolysaccharide to replicate sepsis (Ferkau et al . , Li & Chan ). This test should be run within 4 hours of sampling.…”