Infection by Leishmania amazonensis in mice: A potential model for chronic hypoxia

Araújo, Alexandra Paiva; Arrais-Silva, Wagner Welber; Giorgio, Selma

doi:10.1016/j.acthis.2012.01.007

Cited by 17 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Primary and secondary antibodies did not react with amastigotes isolated from the lesions. Tissue sections were visualized with a peroxidase substrate solution containing 3,3-diaminobenzidine and hydrogen peroxide [7,8] and counterstained with hematoxylin, dehydrated in graded alcohol solutions and mounted in cytoseal-60 mounting medium (Sigma). The extent and intensity of the expression of each marker were Arch Dermatol Res analyzed, and the intensity of staining was scored as positive (strong to weak staining) and negative (absence of staining).…”

Section: Immunochemistrymentioning

confidence: 99%

“…As described previously [7], the lesion tissues from mice killed at designated time points were fixed for 24 h with paraformaldehyde and embedded in paraffin blocks. Tissue sections (5 lm) were deparaffinized and dehydrated, treated with 5 % hydrogen peroxide for 30 min, and washed with PBS.…”

Section: Immunochemistrymentioning

confidence: 99%

“…Thus, similar knowledge could help to determine the relevance of different host mediators in relation to leishmaniasis control or exacerbation and their predictive value. Recently, our group showed that mice cutaneous lesions present hypoxic areas, i.e., low oxygen tension in the diseased tissue [7,9]. In addition, infected macrophages from lesions accumulate HIF-1a and HIF-2a [7].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, our group showed that mice cutaneous lesions present hypoxic areas, i.e., low oxygen tension in the diseased tissue [7,9]. In addition, infected macrophages from lesions accumulate HIF-1a and HIF-2a [7]. Here, we evaluate the kinetics of some important inflammatory molecules which are detected immunohistochemically in the cellular infiltrate of various inflammatory diseases, in self-controlled lesions in C57BL/6 mice and severe lesions in Balb/c mice infected with L. amazonensis, including: p38 mitogen-activated protein kinase (p38 MAPK) and cyclooxygenase-2 (COX-2) in arthritis [15,22], migration inhibitory factor (MIF) in arthrosclerosis [34], macrophage inflammatory protein 2 (MIP-2) in encephalomyelitis [37], and heat shock protein 70 kDa (HSP70) in colitis [62].…”

Section: Introductionmentioning

confidence: 99%

“…Mouse models infected with L. amazonensis, one of the species involved in localized and diffuse cutaneous leishmaniasis in Brazil, have been an important model for studying pathogen interactions with host immune system [7,36,47]. Leishmaniosis has been associated with inflammation because of the involvement of pro-and anti-inflammatory cytokines, such as TNFa, IFNc, IL1b and IL10, in the exacerbation and control of disease [42,45].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

Araújo

Giorgio

2015

Arch Dermatol Res

Self Cite

View full text Add to dashboard Cite

Leishmanioses are chronic parasitic diseases and host responses are associated with pro- or anti-inflammatory cytokines involved, respectively, in the control or exacerbation of infection. The relevance of other inflammatory mediators and stress markers has not been widely studied and there is a need to search for biomarkers to leishmaniasis. In this work, the stress and inflammatory molecules p38 mitogen-activated protein kinase, cyclooxygenase-2, migration inhibitory factor, macrophage inflammatory protein 2, heat shock protein 70 kDa, vascular endothelial factor (VEGF), hypoxia-inducible factors (HIF-1α and HIF-2α), heme oxygenase and galectin-3 expression were assessed immunohistochemically in self-controlled lesions in C57BL/6 mice and severe lesions in Balb/c mice infected with Leishmania amazonensis. The results indicated that the majority of molecules were expressed in the cutaneous lesions of both C57BL/6 and Balb/c mice during various phases of infection, suggesting no obvious correlation between the stress and inflammatory molecule expression and the control/exacerbation of leishmanial lesions. However, the cytokine VEGF was only detected in C57BL/6 footpad lesions and small lesions in Balb/c mice treated with antimonial pentavalent. These findings suggest that VEGF expression could be a predictive factor for murine leishmanial control, a hypothesis that should be tested in human leishmaniosis.

show abstract

Section: Immunochemistrymentioning

confidence: 99%

Section: Immunochemistrymentioning

confidence: 99%