Infection by the nematode Angiostrongylus cantonensis induces differential expression of miRNAs in mouse brain

Mo, Ze Xun; Guo, Jin Qiang; She, Dan; Zhang, Xin; Puthiyakunnon, Santhosh; Chen, Xiao Guang; Wu, Zhong Dao; Shin, Jyh Wei; Cui, Li Wang; Li, Hua

doi:10.1016/j.jmii.2016.02.002

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…miR-155-5p is a well-defined microRNA induced by LPS in macrophages, which enhances the proinflammatory response by targeting the immunomodulatory mRNAs Ship1 , Socs1 , Il13rα , and C/ebpβ and increasing the half-life of Tnf [64, 84–88]. However, antigens of another helminth, Angiostrongylus cantonensis , also upregulated miR-155-5p [89]. Therefore, it would be of interest to further study the role of miR-155-5p during exposure to helminth antigens.…”

Section: Discussionmentioning

confidence: 99%

Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages

Martínez-Saucedo

Ruíz-Rosado

Terrazas

et al. 2019

Journal of Immunology Research

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Helminth parasites modulate immune responses in their host to prevent their elimination and to establish chronic infections. Our previous studies indicate that Taenia crassiceps-excreted/secreted antigens (TcES) downregulate inflammatory responses in rodent models of autoimmune diseases, by promoting the generation of alternatively activated-like macrophages (M2) in vivo. However, the molecular mechanisms triggered by TcES that modulate macrophage polarization and inflammatory response remain unclear. Here, we found that, while TcES reduced the production of inflammatory cytokines (IL-6, IL-12, and TNFα), they increased the release of IL-10 in LPS-induced bone marrow-derived macrophages (BMDM). However, TcES alone or in combination with LPS or IL-4 failed to increase the production of the canonical M1 or M2 markers in BMDM. To further define the anti-inflammatory effect of TcES in the response of LPS-stimulated macrophages, we performed transcriptomic array analyses of mRNA and microRNA to evaluate their levels. Although the addition of TcES to LPS-stimulated BMDM induced modest changes in the inflammatory mRNA profile, it induced the production of mRNAs associated with the activation of different receptors, phagocytosis, and M2-like phenotype. Moreover, we found that TcES induced upregulation of specific microRNAs, including miR-125a-5p, miR-762, and miR-484, which are predicted to target canonical inflammatory molecules and pathways in LPS-induced BMDM. These results suggest that TcES can modulate proinflammatory responses in macrophages by inducing regulatory posttranscriptional mechanisms and hence reduce detrimental outcomes in hosts running with inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages

Martínez-Saucedo

Ruíz-Rosado

Terrazas

et al. 2019

Journal of Immunology Research

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“…The miR-146 had been identified as regulator of inflammation in CNS by interacting with IRAK at 3′UTR and plays role in NF-κβ signaling with the help of adaptor TRAF6 ( Yu et al, 2014a ) and modulates TNF-α expression in mice model of meningitis ( Mo et al, 2016 ). Similarly, higher expression of miR-155, miR-206, miR-223, and miR-511 had been co-related with higher inflammation in mice model of meningitis ( Yu et al, 2014b ).…”

Section: Mirna In Immune Activation: Strategic Decision Makersmentioning

confidence: 99%

Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

et al. 2017

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Helminthic infections fall under neglected tropical diseases, although they inflict severe morbidity to human and causes major economic burden on health care system in many developing countries. There is increased effort to understand their immunopathology in recent days due to their immuno-modulatory capabilities. Immune response is primarily controlled at the transcriptional level, however, microRNA-mediated RNA interference is emerging as important regulatory machinery that works at the translation level. In the past decade, microRNA (miRNA/miR) research has advanced with significant momentum. The result is ever increasing list of curated sequences from a broad panel of organisms including helminths. Several miRNAs had been discovered from trematodes, nematodes and cestodes like let-7, miR155, miR-199, miR-134, miR-223, miR-146, and fhe-mir-125a etc., with potential role in immune modulation. These miRs had been associated with TGF-β, MAPK, Toll-like receptor, PI3K/AKT signaling pathways and insulin growth factor regulation. Thus, controlling the immune cells development, survival, proliferation and death. Apart from micromanagement of immune system, they also express certain unique miRNA also like cis-miR-001, cis-miR-2, cis-miR-6, cis-miR-10, cis-miR-18, cis-miR-19, trs-mir-0001, fhe-miR-01, fhe-miR-07, fhe-miR-08, egr-miR-4988, egr-miR-4989 etc. The specific role played by most of these species specific unique miRs are yet to be discovered. However, these newly discovered miRNAs might serve as novel targets for therapeutic intervention or biomarkers for parasitic infections.

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“…Estos resultados sugieren que el parásito adulto de A. cantonensis libera esta proteína con su función antes descrita; sin embargo, ha sido reportada por tener efectos severos para la modulación de la inmunidad por Th2 30 . Según Ze-Xun, et al 31 la identificación de perfiles de micro ARN (Ácido Ribonucleico) en el Sistema nervioso central (SNC) de ratones infectados por ☞ http://archivosdeneurociencias.com MENINGOENCEFALITIS EOSINOFÍLICA POR ANGIOSTRONGYLUS CANTONENSIS A. cantonensis reveló significativas alteraciones en la expresión de micro ARN. De esta manera se involucra el micro ARN en las respuestas inmunitarias de los ratones infectados y ayuda a comprender los mecanismos moleculares de la respuesta inmune frente a A. cantonensis en hospederos no habituales.…”

Section: Diagnóstico Auxiliarunclassified

“…En la figura 2 se puede apreciar los resultados de los Reibergramas para un grupo de pacientes con meningoencefalitis eosinofílica por Angiostrongylus cantonensis. Kuang-Yao, et al, realizaron un análisis proteómico de los productos de excreción-secreción en adultos jóvenes de A. cantonensis identificándose tres proteínas inmunorreactivas mediante Western Blott: disulfidoisomerasa, putativo aspártica proteasa y anexina, las cuales pueden ser útiles en el diagnóstico y tratamiento de la Meningoencefalitis eosinofílica 31 . Además, se ha empleado a la proteína señalizadora Sonic Hedgehog (Shh) con efectos antiapoptóticos a través de la reducción del estrés oxidativo en astrocitos 36 .…”

Section: Diagnóstico Auxiliarunclassified

Meningoencefalitis eosinofílica por Angiostrongylus cantonensis

Ramos-Robledo,

Meijides-Mejías,

Zamora-Fung

et al. 2020

Arch Neurocien

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Angiostrongylus cantonensis (A. cantonensis) es un helminto que constituye la principal causa de meningoencefalitis eosinofílica en el mundo y endémica en muchas partes del mundo. El objetivo de este trabajo es revisar la literatura publicada sobre el tema sobre el diagnóstico y tratamiento de la enfermedad que ocasiona, la fisiopatología de la enfermedad, casos interesantes y las particularidades de esta donde no existan hábitos de ingerir caracoles crudos y no existan otras etiologías. Se revisaron 37 artículos sobre la enfermedad en revistas nacionales e internacionales de acceso abierto que abarca la base de datos Scopus en el período de 1957 a octubre de 2019. Se confirma que la liberación de sustancias tóxicas, por lisis masiva de las larvas del parásito, produce el proceso inflamatorio en el sistema nervioso central y de esta manera se instaura finalmente la meningoencefalitis eosinofílica. Las manifestaciones clínicas más comunes de la enfermedad incluyen cefalea intensa, rigidez de nuca, náuseas, vómitos y parestesias. La fiebre se presenta en aproximadamente en la tercera parte de los enfermos. Los síntomas y los signos son a menudo leves o moderados, pero pueden llegar el coma. El diagnóstico está basado en los factores epidemiológicos, cuadro clínico presentado, así como los hallazgos positivos de los métodos diagnósticos inmunológicos y por PCR y los reibergramas como auxiliares. El tratamiento presenta alternativas al tratamiento sintomático con la talidomida y albendazol y la terapéutica combinada Interleucina-12 (IL-12) /Albendazol ensayada hasta ahora en ratas.

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Infection by the nematode Angiostrongylus cantonensis induces differential expression of miRNAs in mouse brain

Abstract: This study suggested a critical role of miRNAs in the host defense during A. cantonensis infection, providing new insights into the molecular mechanisms underlying the interaction between mmu-miR-146a-5p and TNF-α in angiostrongyliasis in nonpermissive hosts.

Cited by 6 publications

References 27 publications

Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages

Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages

Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

Meningoencefalitis eosinofílica por Angiostrongylus cantonensis

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