Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference

Liu, Si; Han, Yanhong; Li, Wanxiang; Ding, Shou‐Wei

doi:10.1128/mmbr.00035-22

Cited by 10 publications

(2 citation statements)

References 179 publications

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“…RNAi is a highly conserved post-transcriptional regulatory mechanism that is ubiquitously present in eukaryotes. Biological cells employ this intricate defense strategy to counteract viral invasion [ 35 , 36 ]. In response, viruses have evolved sophisticated RNAi inhibitors to subvert this host defense mechanism and ensure their transcription and replication [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells

Zheng,

Zhao,

Xiong

et al. 2024

Insects

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RNA interference inhibitors were initially discovered in plant viruses, representing a unique mechanism employed by these viruses to counteract host RNA interference. This mechanism has found extensive applications in plant disease resistance breeding and other fields; however, the impact of such interference inhibitors on insect cell RNA interference remains largely unknown. In this study, we screened three distinct interference inhibitors from plant and mammal viruses that act through different mechanisms and systematically investigated their effects on the insect cell cycle and baculovirus infection period at various time intervals. Our findings demonstrated that the viral suppressors of RNA silencing (VSRs) derived from plant and mammal viruses significantly attenuated the RNA interference effect in insect cells, as evidenced by reduced apoptosis rates, altered gene regulation patterns in cells, enhanced expression of exogenous proteins, and improved production efficiency of recombinant virus progeny. Further investigations revealed that the early expression of VSRs yielded superior results compared with late expression during RNA interference processes. Additionally, our results indicated that dsRNA-binding inhibition exhibited more pronounced effects than other modes of action employed by these interference inhibitors. The outcomes presented herein provide novel insights into enhancing defense mechanisms within insect cells using plant and mammal single-stranded RNA virus-derived interference inhibitors and have potential implications for expanding the scope of transformation within insect cell expression systems.

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Section: Discussionmentioning

confidence: 99%

Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells

Zheng,

Zhao,

Xiong

et al. 2024

Insects

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show abstract

“…The role of siRNAs in stress memory: RNA-directed DNA methylation siRNAs are a class of non-coding RNA molecules produced through the processing of long double-stranded RNA (dsRNA) precursors (Lee et al, 2023). Similarly to miRNAs, siRNAs actively participate in RNA interference (RNAi) by binding to complementary mRNA sequences, resulting in mRNA cleavage and subsequent degradation (Liu et al, 2023). Notably, siRNAs can also induce silencing and RNA-directed DNA methylation (RdDM) to enforce epigenetic states, which may be involved in plant stress memory (Liu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The multifaceted role of RNA-based regulation in plant stress memory

Xu,

Cao,

Liu

et al. 2024

Front. Plant Sci.

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Plants have evolved interconnected regulatory pathways which enable them to respond and adapt to their environments. In plants, stress memory enhances stress tolerance through the molecular retention of prior stressful experiences, fostering rapid and robust responses to subsequent challenges. Mounting evidence suggests a close link between the formation of stress memories and effective future stress responses. However, the mechanism by which environmental stressors trigger stress memory formation is poorly understood. Here, we review the current state of knowledge regarding the RNA-based regulation on stress memory formation in plants and discuss research challenges and future directions. Specifically, we focus on the involvement of microRNAs (miRNAs), small interfering RNAs (siRNAs), long non-coding RNAs (lncRNAs), and alternative splicing (AS) in stress memory formation. miRNAs regulate target genes via post-transcriptional silencing, while siRNAs trigger stress memory formation through RNA-directed DNA methylation (RdDM). lncRNAs guide protein complexes for epigenetic regulation, and AS of pre-mRNAs is crucial to plant stress memory. Unraveling the mechanisms underpinning RNA-mediated stress memory formation not only advances our knowledge of plant biology but also aids in the development of improved stress tolerance in crops, enhancing crop performance and global food security.

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Antiviral RNA interference inhibits virus vertical transmission in plants

Liu,

Ding

2024

Cell Host & Microbe

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Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference

Cited by 10 publications

References 179 publications

Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells

Impact of the Transboundary Interference Inhibitor on RNAi and the Baculovirus Expression System in Insect Cells

The multifaceted role of RNA-based regulation in plant stress memory

Antiviral RNA interference inhibits virus vertical transmission in plants

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