Infection during Infancy and Long Incubation Period of Leprosy Suggested in a Case of a Chimpanzee Used for Medical Research

Suzuki, Koichi; Udono, Toshifumi; Fujisawa, Michiko; Tanigawa, Kazunari; Idani, Gen’ichi; Ishii, Norihisa

doi:10.1128/jcm.00017-10

Cited by 38 publications

(36 citation statements)

References 15 publications

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“…PCR primers were designed to be specific to the M. leprae genome and were used in previous studies [24], [27], [28]; all the primers are listed in Table 2. The PCR products were analyzed by 2% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Detection of Mycobacterium leprae DNA from Archaeological Skeletal Remains in Japan Using Whole Genome Amplification and Polymerase Chain Reaction

et al. 2010

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BackgroundIdentification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia.Methodology/Principal FindingsHere we demonstrate Mycobacterium leprae DNA from archaeological skeletal remains in Japan by polymerase chain reaction, DNA sequencing and single nucleotide polymorphism (SNP) analysis. In addition, we have established a highly sensitive method of detecting DNA using a combination of whole genome amplification and polymerase chain reaction, or WGA-PCR, which provides superior sensitivity and specificity in detecting DNA from trace amounts of skeletal materials.Conclusion/SignificanceWe have detected M. leprae DNA in archaeological skeletal remains for the first time in the Far East. Its SNP genotype corresponded to type 1; the first detected case worldwide of ancient M. leprae DNA. We also developed a highly sensitive method to detect ancient DNA by utilizing whole genome amplification.

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Section: Methodsmentioning

confidence: 99%

Detection of Mycobacterium leprae DNA from Archaeological Skeletal Remains in Japan Using Whole Genome Amplification and Polymerase Chain Reaction

et al. 2010

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“…Various incubation periods have been reported: very short ones in young children (3 and 6 months old) [28], or very long ones (up to 30 years) [29]. The short incubation period was observed in 2 leprosy patients with a bacilli count respectively performed 4 months and 15 days before the first signs of lepromatous skin lesions [28].…”

Section: Pathophysiologymentioning

confidence: 99%

Update on the epidemiology, diagnosis, and treatment of leprosy

Reibel

Cambau

Aubry

2015

Médecine et Maladies Infectieuses

121

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“…Furthermore, cases in African chimpanzees raise the possibility that these animals could maintain leprosy transmission in Africa (Suzuki et al 2010). Changes in leprosy magnitude in high-incidence areas will likely only be apparent after many years of work, but it is important to monitor this progress.…”

Section: Discussionmentioning

confidence: 99%

Leprosy frequency in the world, 1999-2010

Penna

2012

Mem. Inst. Oswaldo Cruz

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Infection during Infancy and Long Incubation Period of Leprosy Suggested in a Case of a Chimpanzee Used for Medical Research

Cited by 38 publications

References 15 publications

Detection of Mycobacterium leprae DNA from Archaeological Skeletal Remains in Japan Using Whole Genome Amplification and Polymerase Chain Reaction

Detection of Mycobacterium leprae DNA from Archaeological Skeletal Remains in Japan Using Whole Genome Amplification and Polymerase Chain Reaction

Update on the epidemiology, diagnosis, and treatment of leprosy

Leprosy frequency in the world, 1999-2010

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