Infection Levels of the Microsporidium Larssoniella duplicati in Populations of the Invasive Bark Beetle Ips duplicatus: From Native to New Outbreak Areas

Zimová, Soňa; Resnerová, Karolína; Vanická, Hana; Horák, Jakub; Trombik, Jiří; Kacprzyk, Magdalena; Lindelöw, Åke; Duduman, Mihai‐Leonard; Holuša, Jaroslav

doi:10.3390/f10020131

Cited by 3 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its distribution range covers mainly Eastern European countries and Scandinavia, but also Asian parts of Russia (EFSA 2017). According to Zimová et al (2019), the beetle is currently spreading to Norway spruce (Picea abies) in many European parts, with high outbreak potentials especially where drought events and warm temperatures coincide with Picea abies outside its native range. In that sense, climate change may also lead to an increasing number of I. duplicatus generations per year (Holusa et al 2003).…”

Section: Introductionmentioning

confidence: 99%

A simple PCR-based approach for rapid detection of Ips typographus and Ips duplicatus in the presence of (associated) symbionts and parasites

2020

View full text Add to dashboard Cite

In plant pest diagnosis, Sanger sequencing of marker genes (DNA-barcoding) is the most applied and appropriate method for the identification of insects. Standard PM7/129 of the European and Mediterranean Plant Protection Organization (EPPO) includes a number of primers and PCR protocols for diagnosing insect pests. LCO1490 and HCO2198 primers recommended herein were shown to be excellent tools for amplifying a fragment of the COI gene from a vast range of arthropods. The COI barcoding region is available for thousands of arthropod taxa in public databases and ready-to-use for evolutionary studies. However, we found that LCO1490 and HCO2198 primers are not working for bark beetles of genus Ips. The attempt to amplify this gene fragment from an individual organism using the barcoding primers led to DNA amplification of associated wasps and nematodes, which were apparently vectored by the beetle. Thus, new primers for Ips that bind specifically to another (non-barcoding) region of the COI gene were developed in the past years. These primers were successfully applied in phylogenetic analyses of this genus, resulting in the adverse effect that COI-based Ips phylogenies cannot be expanded to higher systematic categories without sequencing the outgroups (as they are not available in databases yet). Here we provide new primers for Ips that differ significantly from DNA sequences of Ips-associated wasps and nematodes and bind to a COI fragment that largely overlaps with the barcoding region proposed in the EPPO standard. Furthermore, using these primers we developed a quick PCR-based test for detecting Ips duplicatus, a quarantine pest currently emerging in many European countries.

show abstract

Section: Introductionmentioning

confidence: 99%

A simple PCR-based approach for rapid detection of Ips typographus and Ips duplicatus in the presence of (associated) symbionts and parasites

2020

View full text Add to dashboard Cite

show abstract

Overwintering Migration of the Double-Spined Spruce Bark Beetle Ips duplicatus (Sahlberg, 1836) (Coleoptera; Curculionidae)

Davídková

Kleinová

Doležal

2023

Forests

View full text Add to dashboard Cite

The double-spined bark beetle, Ips duplicatus, is an example of a species whose economic importance has increased in many European countries over the past decade. Many aspects of its life cycle remain unknown, complicating its management. I. duplicatus adults overwinter in forest litter, which makes the removal of infested trees during winter ineffective. Therefore, this study focuses on the mechanisms and timing of I. duplicatus overwintering migration into the forest litter. Only 2.2% of adult I. duplicatus overwintered under the bark of spruce logs stored outdoors from August 2016 to March 2017. In all, 94.4% of the beetles emerged and burrowed in forest litter. Migration began in late August, peaked in mid-September, and lasted until mid-October. At that time, forest litter collected at the base of an infested tree contained an average of 154.7 ± 23.9 I. duplicatus. Catches of migrating adults on glue stripes proved that the beetles walked down the trunk on days when the average daily temperature reached 10–15 °C. Based on the results, we suggest that trees infested with I. duplicatus that have completed their development into adults should be removed no later than September. Sanitation cutting in the following months is not effective against this pest.

show abstract

Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae)

Johny,

Große-Wilde,

Kalinová

et al. 2024

IJMS

View full text Add to dashboard Cite

The northern bark beetle, Ips duplicatus, is an emerging economic pest, reportedly infesting various species of spruce (Picea spp.), pine (Pinus spp.), and larch (Larix spp.) in Central Europe. Recent climate changes and inconsistent forest management practices have led to the rapid spread of this species, leaving the current monitoring strategies inefficient. As understanding the molecular components of pheromone detection is key to developing novel control strategies, we generated antennal transcriptomes from males and females of this species and annotated the chemosensory proteins. We identified putative candidates for 69 odorant receptors (ORs), 50 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 27 odorant-binding proteins (OBPs), including a tetramer-OBP, 9 chemosensory proteins (CSPs), and 6 sensory neuron membrane proteins (SNMPs). However, no sex-specific chemosensory genes were detected. The phylogenetic analysis revealed conserved orthology in bark beetle chemosensory proteins, especially with a major forest pest and co-habitant, Ips typographus. Recent large-scale functional studies in I. typographus chemoreceptors add greater significance to the orthologous sequences reported here. Nevertheless, identifying chemosensory genes in I. duplicatus is valuable to understanding the chemosensory system and its evolution in bark beetles (Coleoptera) and, generally, insects.

show abstract

Infection Levels of the Microsporidium Larssoniella duplicati in Populations of the Invasive Bark Beetle Ips duplicatus: From Native to New Outbreak Areas

Cited by 3 publications

References 30 publications

A simple PCR-based approach for rapid detection of Ips typographus and Ips duplicatus in the presence of (associated) symbionts and parasites

A simple PCR-based approach for rapid detection of Ips typographus and Ips duplicatus in the presence of (associated) symbionts and parasites

Overwintering Migration of the Double-Spined Spruce Bark Beetle Ips duplicatus (Sahlberg, 1836) (Coleoptera; Curculionidae)

Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae)

Contact Info

Product

Resources

About