Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Su, Ang; Tong, Jie; Fu, Yue; Müller, Sandy; Weldearegay, Yenehiwot Berhanu; Becher, Paul; Valentin‐Weigand, Peter; Meens, Jochen; Herrler, Georg

doi:10.1186/s13567-020-00861-2

Cited by 11 publications

(16 citation statements)

References 44 publications

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“…In addition, at this inoculation density, cilia coverage of the ALI at 24 hpi was significantly lower than in the control cultures with no bacteria . The authors concluded that this observation could be due to the lack of mucus-clearing in the ALI model compared to in vivo conditions …”

Section: In Vitro Respiratory System Modelsmentioning

confidence: 83%

Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease

2023

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Bovine respiratory disease (BRD) is a multifactorial condition where different genera of bacteria, such as Mannheimia haemolytica, Histophilus somni, Pasteurella multocida, and Mycoplasma bovis, and viruses, like bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes virus-1, infect the lower respiratory tract of cattle. These pathogens can co-infect cells in the respiratory system, thereby making specific treatment very difficult. Currently, the most common models for studying BRD include a submerged tissue culture (STC), where monolayers of epithelial cells are typically covered either in cellular or spent biofilm culture medium. Another model is an air−liquid interface (ALI), where epithelial cells are exposed on their apical side and allowed to differentiate. However, limited work has been reported on the study of three-dimensional (3D) bovine models that incorporate multiple cell types to represent the architecture of the respiratory tract. The roles of different defense mechanisms in an infected bovine respiratory system, such as mucin production, tight junction barriers, and the production of antimicrobial peptides in in vitro cultures require further investigation in order to provide a comprehensive understanding of the disease pathogenesis. In this report, we describe the different aspects of BRD, including the most implicated pathogens and the respiratory tract, which are important to incorporate in disease models assembled in vitro. Although current advancements of bovine respiratory cultures have led to knowledge of the disease, 3D multicellular organoids that better recapitulate the in vivo environment exhibit potential for future investigations.

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Section: In Vitro Respiratory System Modelsmentioning

confidence: 83%

Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease

2023

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“…In addition, they are also considered one of the secondary exacerbation factors of BRD. Moreover, it is known that Pm and Mh were further infected in nasal mucosa when damaged by stress and/or viruses, and therefore, symptoms of the infected animals became more severe [ 39 , 40 ]. Our analysis exhibited that there were no significant differences in the detection of Mh- and Pm-specific genes between BRD-infected and healthy calves.…”

Section: Discussionmentioning

confidence: 99%

Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves

Goto

Fukunari

Suzuki

2023

Viruses

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Bovine respiratory diseases (BRD) are associated with various predisposing factors, such as physical and physiological stress factors, and bacterial and viral pathogens. These stressors and viruses suppress immune defenses, leading to bacterial growth in the upper respiratory tract and invasion of pathogens into the lower respiratory tract. Therefore, continuous monitoring of the causative pathogens would contribute to the early detection of BRD. Nasal swabs and sera from 63 clinically healthy calves were continuously collected from seven farms in Iwate prefecture from 2019 to 2021. We attempted to monitor dynamics of BRD-associated pathogens by multiplex real-time RT-PCR (RT-qPCR) using their nasal swab samples. In addition, we attempted to monitor fluctuation of antibody titers against each BRD-associated pathogen by virus neutralization test (VNT) using their sera. In contrast, nasal swabs from 89 calves infected with BRD were collected from 28 farms in Iwate prefecture from 2019 to 2021. We attempted to analyze their nasal swab samples by multiplex RT-qPCR aim to detect BRD-associated pathogens that are dominant in this region. As a result, our analyses using samples from clinically healthy calves showed that positive results by multiplex RT-qPCR were closely related to a significant increase of antibody titers by VNT in bovine coronavirus (BCoV), bovine torovirus (BToV), and bovine respiratory syncytial virus (BRSV). In addition, our data exhibited that BCoV, BToV, BRSV, bovine parainfluenza virus 3, and Mycoplasma bovis have been more frequently detected in calves infected with BRD compared to those detected in clinically healthy calves. Moreover, the data presented herein revealed co-infections by combination multiple viral pathogens with bacterial pathogens are closely involved in the onset of BRD. Taken together, our study demonstrates multiplex RT-qPCR which can simultaneously analyze multiple pathogens, including viruses and bacteria, and is useful for the early detection of BRD.

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“…It causes pneumonia, genital infections, abscesses and septicemia. Thus, leads to shortage in production and high mortality rate (Su et al 2020). The current study was designed to evaluate the expression and immunogenicity of recombinant outer membrane protein A (OmpA) of P. multocida.…”

Section: Discussionmentioning

confidence: 99%

The potentials of Pasteurella multocida OmpA protein as the candidate for sub-unit vaccine and for the development of an ELISA kit to evaluate the vaccine response in the animals

Shoaib

Nawaz

Absar

et al. 2023

Preprint

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Pasteurella multocida is the primary causative agent of Hemorrhagic Septicemia (HS) in cattle and Buffalo. It is responsible for causing huge economic loses every year. The P. multocida vaccines are used for livestock, but because sensitive and specific serological tests are not available, sero-conversion in these animals is unknown. As a result, the vaccine and it’s the immune response cannot be evaluated. So, the present study was designed to evaluate immunogenic potential of OmpA gene of P. multocida. PCR primers designed to have restriction sites to cut amplicon and ligate product into expression vector. Purified PCR product was subjected to cloning PCR and the product was sequenced. The product was excised from cloning vector and ligated into expression vector (pET 40b (+)). Expression vector was transformed to chemically competent Escherichia coli strain DH5-α by heat shock method. Plasmid was extracted and ligation was confirmed by restriction digestion. IPTG was used to trigger expression. Total cell protein and medium were SDS-PAGE was used to analyze the expression. Recombinant proteins were injected into mice to test their immunogenicity. OmpA was proved to be highly immunogenic in nature, making it a more suitable candidate for sub-unit vaccine preparation and development of ELISA kit for the detection of immune responses in animals.

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Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Cited by 11 publications

References 44 publications

Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease

Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease

Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves

The potentials of Pasteurella multocida OmpA protein as the candidate for sub-unit vaccine and for the development of an ELISA kit to evaluate the vaccine response in the animals

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