Infection of Colletotrichum acutatum and Phytophthora infestans by taxonomically different plant viruses

Mascia, Tiziana; Vučurović, Ana; Minutillo, S. A.; Nigro, Franco; Labarile, Rossella; Savoia, Michele Antonio; Palukaitis, Peter; Gallitelli, D.

doi:10.1007/s10658-018-01615-9

Cited by 23 publications

(16 citation statements)

References 42 publications

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“…2019 ). Evidence of cross-kingdom HVT was indeed shown for plant viruses replicating in fungi both experimentally ( Mascia et al. 2019 ) and naturally ( Andika et al.…”

Section: Introductionmentioning

confidence: 90%

The virome from a collection of endomycorrhizal fungi reveals new viral taxa with unprecedented genome organization

et al. 2020

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Mutualistic plant-associated fungi are recognized as important drivers in plant evolution, diversity and health. The discovery that mycoviruses can take part and play important roles in symbiotic tripartite interactions has prompted us to study the viromes associated with a collection of ericoid and orchid mycorrhizal (ERM and ORM, respectively) fungi. Our study, based on high-throughput sequencing of transcriptomes (RNAseq) from fungal isolates grown in axenic cultures, revealed in both ERM and ORM fungi the presence of new mycoviruses closely related to already classified virus taxa, but also new viruses that expand the boundaries of characterized RNA virus diversity to previously undescribed evolutionary trajectories. In ERM fungi, we provide first evidence of a bipartite virus, distantly related to narnaviruses, that splits the RNA-dependent RNA polymerase (RdRP) palm domain into two distinct proteins, encoded by each of the two segments. Furthermore, in one isolate of the ORM fungus Tulasnella spp. we detected a 12 kb genomic fragment coding for an RdRP with features of bunyavirus-like RdRPs. However, this 12 kb genomic RNA has the unique features, for Bunyavirales members, of being tri-cistronic and carrying ORFs for the putative RdRP and putative nucleocapsid in ambisense orientation on the same genomic RNA. Finally, a number of ORM fungal isolates harbored a group of ambisense bicistronic viruses with a genomic size of around 5 kb, where we could identify a putative RdRP palm domain that has some features of plus strand RNA viruses; these new viruses may represent a new lineage in the Riboviria, as they could not be reliably assigned to any of the branches in the recently derived monophyletic tree that includes most viruses with an RNA genome.

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“…2019 ). Evidence of cross-kingdom HVT was indeed shown for plant viruses replicating in fungi both experimentally ( Mascia et al. 2019 ) and naturally ( Andika et al.…”

Section: Introductionmentioning

confidence: 90%

The virome from a collection of endomycorrhizal fungi reveals new viral taxa with unprecedented genome organization

et al. 2020

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“…Indeed, there are a number of examples of the successful or promising use of mycoviruses to control fungal diseases (37)(38)(39). Interestingly, a number of studies have recently demonstrated that phytopathogenic fungi could be a suitable host of some plant viruses (40)(41)(42), which extends the previously known compatibility of plant viruses and yeast (S. cerevisiae) as hosts (43,44). Conversely, mycoviruses originating from marine fungi (Penicillium aurantiogriseum var.…”

Section: Significancementioning

confidence: 96%

Symptomatic plant viroid infections in phytopathogenic fungi

Wei

Bian

Andika

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Viroids are pathogenic agents that have a small, circular noncoding RNA genome. They have been found only in plant species; therefore, their infectivity and pathogenicity in other organisms remain largely unexplored. In this study, we investigate whether plant viroids can replicate and induce symptoms in filamentous fungi. Seven plant viroids representing viroid groups that replicate in either the nucleus or chloroplast of plant cells were inoculated to three plant pathogenic fungi, Cryphonectria parasitica, Valsa mali, and Fusarium graminearum. By transfection of fungal spheroplasts with viroid RNA transcripts, each of the three, hop stunt viroid (HSVd), iresine 1 viroid, and avocado sunblotch viroid, can stably replicate in at least one of those fungi. The viroids are horizontally transmitted through hyphal anastomosis and vertically through conidia. HSVd infection severely debilitates the growth of V. mali but not that of the other two fungi, while in F. graminearum and C. parasitica, with deletion of dicer-like genes, the primary components of the RNA-silencing pathway, HSVd accumulation increases. We further demonstrate that HSVd can be bidirectionally transferred between F. graminearum and plants during infection. The viroids also efficiently infect fungi and induce disease symptoms when the viroid RNAs are exogenously applied to the fungal mycelia. These findings enhance our understanding of viroid replication, host range, and pathogenicity, and of their potential spread to other organisms in nature.plant viroid | fungus | transmission | pathogenicity V iroids are infectious pathogenic agents possessing small, nonencapsidated, circular single-stranded RNAs that, to date, have been found to naturally infect only plants (1, 2). Viroids infect a wide variety of higher plant species, causing devastating diseases in many crops, particularly vegetables, fruits, and ornamental plants (3). In crop plants, viroids are known to spread by vegetative propagation; by mechanical agricultural practices; and, in certain cases, through seeds, pollen, and insect transmission (3, 4). As viroids do not encode any proteins and do not require a helper agent for their multiplication and survival, the biological activities of viroids are entirely dependent on direct interactions of their RNA genome or its derivatives with cellular host components (5-9). Viroid replication or processing of its RNAs in the yeast, Saccharomyces cerevisiae, and cyanobacterium, Nostoc (Nostocales), have been demonstrated (10-12).Currently, at least 34 viroid species have been identified and are classified into two families, Avsunviroidae and Pospiviroidae (13-15). The members of Avsunviroidae (type species: Avocado sunblotch viroid) replicate in the chloroplasts or plastids through symmetric rolling-circle replication using the host nuclear-encoded polymerase. Their RNAs form highly branched secondary structures and have ribozyme activities. Members of Pospiviroidae (type species: Potato spindle tuber viroid) replicate and accumulate in the nucleus th...

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“…Furthermore, horizontal transmission of cucumber mosaic virus (CMV) was observed in the phytopathogenic fungus Rhizoctonia solani [98]. Far less is known about the mycoviruses that infect, and replicate in, plant cells [99,100]. Notably, the fungal virus CHV1 was only able to systemically infect Nicotiana tabacaum plants when inoculated together with other plant viruses or tobacco mosaic virus (TMV), which indicates facilitative interactions between fungi and plant viruses [101].…”

Section: Horizontal Gene Transfer Has Revealed Foreign Structural Dommentioning

confidence: 99%

Viruses of endophytic and pathogenic forest fungi

Siddique

2020

Virus Genes

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Mycoviruses, just as the fungal endophytes they infect, are ubiquitous biological entities on Earth. Mycoviruses constitute a diverse group of viruses, and metagenomic approaches have-through recent discoveries of been mycoviruses-only recently began to provide evidence of this astonishing diversity. The current review presents (1) various mycoviruses which infect fungal endophytes and forest pathogens, (2) their presumed origins and interactions with fungi, plants and the environment, (3) high-throughput sequencing techniques that can be used to explore the horizontal gene transfer of mycoviruses, and (4) how the hypo-and hypervirulence induced by mycoviral infection is relevant to the biological control of pathogenic fungi.

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Infection of Colletotrichum acutatum and Phytophthora infestans by taxonomically different plant viruses

Cited by 23 publications

References 42 publications

The virome from a collection of endomycorrhizal fungi reveals new viral taxa with unprecedented genome organization

The virome from a collection of endomycorrhizal fungi reveals new viral taxa with unprecedented genome organization

Symptomatic plant viroid infections in phytopathogenic fungi

Viruses of endophytic and pathogenic forest fungi

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