Infection of Escherichia coli K-12 by bacteriophage φX-174

Dowell, C. E.; Jansz, H.S.; Zandberg, Jaco

doi:10.1016/0042-6822(81)90271-3

Cited by 14 publications

(11 citation statements)

References 6 publications

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“…While the JACS-K strain was capable of forming plaques on both E. coli host plates, the ancestral JACS strain was not. The JACS-K plaques on E. coli K-12 were significantly smaller than the plaques observed on the E. coli C plates, similar to those described previously (7). Over 40 replicates were conducted to ensure reproducibility of the mutant's infectivity of E. coli K-12 and plaque size.…”

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confidence: 75%

Mechanisms Responsible for a ΦX174 Mutant's Ability To InfectEscherichia coliby Phosphorylation

Cox

Putonti

2010

J Virol

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The ability for a virus to expand its host range is dependent upon a successful mode of viral entry. As such, the host range of the well-studied ΦX174 bacteriophage is dictated by the presence of a particular lipopolysaccharide (LPS) on the bacterial surface. The mutant ΦX174 strain JACS-K, unlike its ancestor, is capable of infecting both its native host Escherichia coli C and E. coli K-12, which does not have the necessary LPS. The conversion of an alanine to a very reactive threonine on its virion surface was found to be responsible for the strain's expanded host range.

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mentioning

confidence: 75%

Mechanisms Responsible for a ΦX174 Mutant's Ability To InfectEscherichia coliby Phosphorylation

Cox

Putonti

2010

J Virol

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“…In early studies, host recognition sites for φX174 and S13 were mapped genetically to the pilot protein H (Sinsheimer 1968), the major spike protein G (Newbold & Sinsheimer 1970; Weisbeek et al . 1973) and the major capsid protein F (Tessman 1965; Dowell et al . 1981), but the identities of host-specific mutations within these proteins are unknown.…”

Section: Resultsmentioning

confidence: 99%

Experimental evolution of viruses: Microviridae as a model system

Wichman

Brown

2010

Phil. Trans. R. Soc. B

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φX174 was developed as a model system for experimental studies of evolution because of its small genome size and ease of cultivation. It has been used extensively to address statistical questions about the dynamics of adaptive evolution. Molecular changes seen during experimental evolution of φX174 under a variety of conditions were compiled from 10 experiments comprising 58 lineages, where whole genomes were sequenced. A total of 667 substitutions was seen. Parallel evolution was rampant, with over 50 per cent of substitutions occurring at sites with three or more events. Comparisons of experimentally evolved sites to variation seen among wild phage suggest that at least some of the adaptive mechanisms seen in the laboratory are relevant to adaptation in nature. Elucidation of these mechanisms is aided by the availability of capsid and pro-capsid structures for φX174 and builds on years of genetic studies of the phage life history.

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“…As we have discussed above, populations comprising different clusters can persist and diverge only if they exist in niches that are sufficiently different to escape purging through periodic selection events. It is possible that the transfer of gene F allowed the two sister clades to occupy sufficiently different niches because gene F encodes the major capsid protein, a known determinant of host specificity in X174 (9,12) and because the host ranges of these phages are different. It is pertinent to this hypothesis that we failed to isolate close relatives of ␣3 and K on E. coli C and that both K and ␣3 were isolated on other E. coli host strains (18).…”

Section: Discussionmentioning

confidence: 99%

Horizontal Gene Transfer and the Evolution of Microvirid Coliphage Genomes

et al. 2006

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Bacteriophage genomic evolution has been largely characterized by rampant, promiscuous horizontal gene transfer involving both homologous and nonhomologous source DNA. This pattern has emerged through study of the tailed double-stranded DNA (dsDNA) phages and is based upon a sparse sampling of the enormous diversity of these phages. The single-stranded DNA phages of the family Microviridae, including X174, appear to evolve through qualitatively different mechanisms, possibly as result of their strictly lytic lifestyle and small genome size. However, this apparent difference could reflect merely a dearth of relevant data. We sought to characterize the forces that contributed to the molecular evolution of the Microviridae and to examine the genetic structure of this single family of bacteriophage by sequencing the genomes of microvirid phage isolated on a single bacterial host. Microvirids comprised 3.5% of the detectable phage in our environmental samples, and sequencing yielded 42 new microvirid genomes. Phylogenetic analysis of the genes contained in these and five previously described microvirid phages identified three distinct clades and revealed at least two horizontal transfer events between clades. All members of one clade have a block of five putative genes that are not present in any member of the other two clades. Our data indicate that horizontal transfer does contribute to the evolution of the microvirids but is both quantitatively and qualitatively different from what has been observed for the dsDNA phages.

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Infection of Escherichia coli K-12 by bacteriophage φX-174

Cited by 14 publications

References 6 publications

Mechanisms Responsible for a ΦX174 Mutant's Ability To InfectEscherichia coliby Phosphorylation

Mechanisms Responsible for a ΦX174 Mutant's Ability To InfectEscherichia coliby Phosphorylation

Experimental evolution of viruses: Microviridae as a model system

Horizontal Gene Transfer and the Evolution of Microvirid Coliphage Genomes

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