Infection of triatomines with Trypanosoma cruzi

Garcia, Elói S.; Azambuja, Patrícia

doi:10.1007/978-94-009-1535-0_13

Cited by 12 publications

(10 citation statements)

References 11 publications

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“…66,67 We placed each jar of nymphs under an artificial membrane feeder containing the parasite-blood solution at 37.5°C for about 30 minutes. 68 Control insects consumed blood without parasites. We weighed each nymph after feeding to estimate the number of parasites ingested, which was calculated by subtracting each insect's pre-feeding weight from its postfeeding weight, dividing this amount by the average density of human blood (1.06 mg/mL), and multiplying the result by 3.3-3.5 × 10 6 flagellates per milliliter, depending on parasite treatment.…”

Section: Methodsmentioning

confidence: 99%

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Peterson

Graham

Dobson

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The effect of a parasite on the life history of its vector is important for understanding and predicting disease transmission. Chagas disease agent Trypanosoma cruzi is a generalist parasite that is diverse across scales from its genetic diversity to the 100s of mammal and vector species it infects. Its vertebrate hosts show quite variable responses to infection, however, to date there are no studies looking at how T. cruzi variability might result in variable outcomes in its invertebrate host. Therefore, we investigated the effect of different T. cruzi I strains on Rhodnius prolixus survival and development. We found significant variation between insects infected with different strains, with some strains having no effect, as compared with uninfected insects, and others with significantly lower survival and development. We also found that different variables had varying importance between strains, with the effect of time postinfection and the blood:weight ratio of the infective meal significantly affecting the survival of insects infected with some strains, but not others. Our results suggest that T. cruzi can be pathogenic not only to its vertebrate hosts but also to its invertebrate hosts.

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Section: Methodsmentioning

confidence: 99%

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Peterson

Graham

Dobson

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Specimens that were completely desiccated at the time of processing were excluded. For specimens collected during field studies, the digestive tract was removed (Garcia and De Azambuja 1997) using sterile forceps and scalpel, and placed in 50 μ l phosphate-buffered saline. Samples were flash-frozen in an ethanol + dry ice bath and stored on dry ice until transported to the laboratory, where they were stored at –70°C until processed.…”

Section: Methodsmentioning

confidence: 99%

Identification of Bloodmeal Sources andTrypanosoma cruziInfection in Triatomine Bugs (Hemiptera: Reduviidae) From Residential Settings in Texas, the United States

et al. 2013

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The host–vector–parasite interactions in Chagas disease peridomestic transmission cycles in the United States are not yet well understood. Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) infection prevalence and bloodmeal sources were determined for adult and immature triatomine (Hemiptera: Reduviidae) specimens collected from residential settings in central Texas. Sequenced cytochrome b DNA segments obtained from triatomine digestive tract identified nine vertebrate hosts and one invertebrate host in four triatomine species (Triatoma gerstaeckeri, Triatoma indictiva, Triatoma protracta, and Triatoma sanguisuga). The broad range of wild and domestic host species detected in triatomine specimens collected from residential sites indicates high host diversity and potential movement between the sylvatic and peridomestic settings. Domestic dogs appear to be key in the maintenance of the peridomestic transmission cycle as both a blood host for the triatomine vectors and a potential reservoir for the parasite. The high rate of T. cruzi infection among triatomine specimens that were collected from inside houses, outside houses, and dog kennels (69, 81, and 82%, respectively) suggests a current risk for Chagas disease vector-borne transmission for humans and domestic animals in residential settings in Texas because of overlap with the sylvatic cycle.

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“…The posterior mid-and hindgut from each newly collected bug was dissected following previously described methods (Garcia and De Azambuja 1997) using sterile, disposable scalpels and forceps disinfected with a 10% bleach solution and ultrapure water rinse between samples. The excised gut was placed in 50-L of molecular-grade water and homogenized using a sterile, disposable pestle.…”

Section: Detection Of T Cruzi In New Specimensmentioning

confidence: 99%

Biogeography andTrypanosoma cruziInfection Prevalence of Chagas Disease Vectors in Texas, USA

Kjos

Snowden

Olson

2009

Vector-Borne and Zoonotic Diseases

118

107

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Data were pooled from multiple sources including newly collected triatomine specimens, preserved specimens, government reports, and scientific articles to create a biogeographical profile of triatomine vector species found in Texas. Triatomine specimens were documented in 97 of 254 counties, and Trypanosoma cruzi-infected specimens were reported from 48 counties. Triatomine specimens were distributed in 11 of the 12 ecoregions in Texas, with all but one species found in multiple ecoregions. Of the 241 newly collected specimens, 50.74% were infected with T. cruzi. Triatoma gerstaeckeri was the most frequently collected and most geographically dispersed species followed by T. sanguisuga. Three species, T. gerstaeckeri, T. sanguisuga, and T. lecticularia, were associated with human dwellings, and over half of the new specimens found inside or near houses were infected with T. cruzi. Chagas disease vectors in Texas are widely distributed and have adapted to ecologically diverse settings. The high T. cruzi infection prevalence of specimens found in close proximity to human settings suggests the presence of an active peridomestic Chagas disease transmission cycle.

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Infection of triatomines with Trypanosoma cruzi

Cited by 12 publications

References 11 publications

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Identification of Bloodmeal Sources andTrypanosoma cruziInfection in Triatomine Bugs (Hemiptera: Reduviidae) From Residential Settings in Texas, the United States

Biogeography andTrypanosoma cruziInfection Prevalence of Chagas Disease Vectors in Texas, USA

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