Infection prevalence and phylogenetic analysis of Perkinsus olseni in Ruditapes philippinarum from East China

Abstract

The prevalence of Perkinsus sp. infection in Manila clam Ruditapes philippinarum was investigated in the coastal areas of east China. Thirteen groups of clams were collected from 5 sites: Dandong and Qingdao Bays (Yellow Sea), Weifang Bay (Bohai Sea), and Ningbo and Fuzhou Bays (East China Sea). The clams were tested for perkinsosis infection using Ray's fluid thioglycollate medium culture assay. Perkinsus sp. was found in samples from all 5 sites from May 2008 to May 2009. Infection prevalence ranged from 43.75 to 95.83%, and was significantly higher in October than in May. The only 3 uninfected groups of clams were collected from Weifang Bay, the site farthest from the ocean. There was no difference in the prevalence of infection among the remaining 4 sites. The conserved internal transcribed spacer regions of the ribosomal RNA gene complex in each of the Perkinsus sp. isolates were amplified by PCR. The resulting amplicons were sequenced and phylogenetically analyzed. All the Perkinsus isolates were identified as Perkinsus olseni. KEY WORDS: Perkinsus olseni · Ruditapes philippinarum · Infection prevalence · Phylogenetic analysis · ChinaResale or republication not permitted without written consent of the publisher

Survey on <i>Perkinsus</i> Species in Manila Clam <i>Ruditapes philippinarum</i> in Korean Waters Using Species-Specific PCR

“…KX514084 and KX514085). ITS sequences of the control P. olseni from Gomso Bay showed 100% sequence similarity to the ITS sequences of P. olseni reported from Portugal (Robledo et al, 2002), Spain (Casas et al, 2002), China (Wu et al, 2011), and Japan (Dungan and Reece, 2006).…”

Development of a loop-mediated isothermal amplification method for detection of Perkinsus spp. in mollusks

“…After shucking, part of the gill was excised and stored at −20°C for later PCR examination. The remaining parts of each clam tissue were cultured separately with RFTM following the methods of Wu et al (2011). In brief, the remaining clam tissue was homogenized and then placed in fluid thioglycollate medium (Fluka) supplemented with 2.5% chloromycetin (Amresco) and 1% nystatin (Amresco).…”

Protein expression profiling in haemocytes and plasma of the Manila clam Ruditapes philippinarum in response to infection with Perkinsus olseni

“…R. philippinarum was introduced into European coast for aquaculture purposes (Gosling 2003); its introduction probably brought new diseases for European clams, such as infection with Perkinsus olseni (Vilas et al 2011). This protistan parasite has been blamed for Manila clam high mortality in Spain (Santmart ı et al 1995) Korea (Park & Choi 2001;Choi & Park 2010), China (Liang et al 2001;Wu et al 2011) and Italy (Pretto et al 2014). The pathogenicity of this parasite in the Manila clam has been demonstrated (Shimokawa, Yoshinaga & Ogawa 2010;Waki et al 2012;Waki & Yoshinaga 2013), and sublethal effects, such as reduction of host fecundity (Park, Figueras & Choi 2006;Uddin et al 2010;Casas & Villalba 2012), may also involve economic losses.…”