Infection with an Acinetobacter-like Bacterium in Atlantic Salmon (Salmo salar) Broodfish

Roald, Sverre Ola; Håstein, Tore

doi:10.1007/978-3-642-67854-7_25

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…Acinetobacter is widely distributed in the external environment and can be isolated in water, soil, humans, and animals [2], but it is generally considered to be rarely pathogenic [32,33]. Te species of Acinetobacter can cause infectious diseases, which is known as a typical opportunistic pathogen in fshes [34,35]. Te damage that Acinetobacter causes to fsh displayed symptoms such as ascites and ulceration on the entire body resembling the septicaemic form of infection [9,36].…”

Section: Discussionmentioning

confidence: 99%

Identification and Pathogenicity of Emerging Fish Pathogen Acinetobacter johnsonii from a Disease Outbreak in Rainbow Trout (Oncorhynchus mykiss)

Yuan

Jia

et al. 2023

Aquaculture Research

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In November 2017, a group of farmed rainbow trout (Oncorhynchus mykiss) died in Yunnan Province, China, likely due to infection. These rainbow trout exhibited slow movement, no feeding, dark body color, exophthalmia, and occasional ulcers on the body surface. Pathogens were isolated from diseased rainbow trout livers, head kidneys, spleens, and eyes, and the strain was preliminarily identified as Acinetobacter johnsonii based on morphology, biochemical tests, and 16S rRNA and rpoB sequences. The isolated strains were highly sensitive to florfenicol, ciprofloxacin, oxolinic acid, and norfloxacin but resistant to ampicillin, doxycycline, sulfadiazine, thiamphenicol, and sulfamethazine. The selected isolate was performed for the experimental infection of rainbow trout to confirm its pathogenicity. Experimentally infected fish showed disease symptoms similar to those observed in fish naturally infected with these bacteria. Vacuolar degeneration was prevalent in the liver and spleen of diseased fish. Cytoplasm volume was very high in the lymphocytes of the head kidney. The protein structure and topology of monomeric outer membrane protein A (OmpA), outer membrane protein 34 (Omp34), and a nucleoside-specific outer membrane transporter protein Tsx (OmpTsx) of A. johnsonii were predicted by AlphaFold 2. The predicted local distance difference test (pLDDT) score was used for the assessment of structure prediction. Comprehensive analysis of antigenic determinants of the OMP family member, OmpA, contains three antigenic determinants, which are 90% similar with epitopes of A. baumannii. OmpA is a recombinant protein with 35 kDa expressed in the Escherichia coli system. Based on these findings, A. johnsonii is regarded as an emerging opportunistic pathogen in farmed rainbow trout. OmpA protein can be used as a subunit vaccine candidate molecule of A. johnsonii.

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Section: Discussionmentioning

confidence: 99%

Identification and Pathogenicity of Emerging Fish Pathogen Acinetobacter johnsonii from a Disease Outbreak in Rainbow Trout (Oncorhynchus mykiss)

Yuan

Jia

et al. 2023

Aquaculture Research

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“…These genera are Acinetobacter , Aeromonas , Chryseobacterium , Corynebacterium , Flavobacterium , Janthinobacterium , Micrococcus , Pseudomonas , Shewanella and Staphylococcus . Bacteria of the genus Acinetobacter sp., as Acinetobacter junii , and Acinetobacter lwoffii, can cause the acinetobacter disease which killed 92% of wild Atlantic Salmon in Norway (Roald & Hastein, ). Aeromonas hydrophila, Aeromonas salmonicida and Aeromonas sobria are known to cause septicaemia or ulcer disease in many freshwater fishes (Austin & Austin, ).…”

Section: Discussionmentioning

confidence: 99%

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Sevellec

Pavey

Boutin

et al. 2014

J of Evolutionary Biology

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Few studies have applied NGS methods to investigate the microbiome of vertebrates in their natural environment and in freshwater fishes in particularly. Here, we used pyrosequencing of the 16S gene rRNA to (i) test for differences in kidney bacterial communities (i.e. microbiota) of dwarf and normal whitefish found as sympatric pairs, (ii) test the hypothesis of higher bacterial diversity in normal compared with dwarf whitefish and (iii) test for the occurrence of parallelism with the presence and composition of bacterial communities across species pairs inhabiting different lakes. The kidney microbiota of 253 dwarf and normal whitefish from five lakes was analysed combining a double-nested PCR approach with 454 pyrosequencing. Bacteria were detected in 52.6% of the analysed whitefish. There was no overall significant difference among lakes and forms, although the lake 9 form interaction was found significant. We identified 579 bacterial genera, which is substantially more than previous descriptions using less sensitive techniques of fish bacterial diversity in kidney, pathogenic or not. Ten of these genera contained eighteen pathogenic species. Differences in bacteria composition between whitefish forms were not parallel among lakes. In accordance with the higher diversity of prey types, normal whitefish kidney tissue consistently had a more diverse bacterial community and this pattern was parallel among lakes. These results add to building evidence from previous studies on this system that the adaptive divergence of dwarf, and normal whitefish has been driven by both parallel and nonparallel ecological conditions across lakes.

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“…Although epizootics caused by these bacteria have not been described in our country, infections by Streptococcus and Staphylococcus produced major losses in fish cultured in Japan (Kusuda and Sugiyama, 1981;Nakasugawa, 1983), the USA (Baya et al, 1990a) and South Africa (Bragg and Broere, 1986). Similarly the presence of MoraxellaAcinetobacter in turbot cannot be ruled out since strains of this group have been implicated in mortalities of Atlantic salmon (Salmo salar L.) in Norway (Roal and Hastein, 1980) and striped bass (Morone saxatilis) in the USA (Baya et al, 1990b).…”

Section: Resultsmentioning

confidence: 99%

Epizootic study in a turbot farm: bacteriology, virology, parasitology and histology

Novoa¹,

Nún˜ez²,

Fernández-Puentes³

et al. 1992

Aquaculture

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Infection with an Acinetobacter-like Bacterium in Atlantic Salmon (Salmo salar) Broodfish

Cited by 9 publications

References 4 publications

Identification and Pathogenicity of Emerging Fish Pathogen Acinetobacter johnsonii from a Disease Outbreak in Rainbow Trout (Oncorhynchus mykiss)

Identification and Pathogenicity of Emerging Fish Pathogen Acinetobacter johnsonii from a Disease Outbreak in Rainbow Trout (Oncorhynchus mykiss)

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Epizootic study in a turbot farm: bacteriology, virology, parasitology and histology

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