Infection with methicillin-gentamicin-resistant Staphylococcus aureus strains in a paediatric surgical unit in Lisbon

Cristino, José A. G. M.; Pereira, A. Torres; Afonso, Fernando

doi:10.1016/0195-6701(85)90060-x

Cited by 9 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hospital receives patients from a tertiary care hospital in Lisbon and from all district hospitals located in the southern part of the country (covering approximately 50% of the territory). The first MRSA isolate was detected in this hospital in 1983 (27), and the prevalence of MRSA was high in 1992 (46.9%), 1993 (44.9%), and 1994 (41.0%). In 1994, drastic infection control measures were introduced into the hospital, which included routine cleaning of all areas, new rules on the use of disinfectants and antiseptics, and control of prescription of antimicrobial agents; frequent hand washing and the use of gloves and gowns were enforced.…”

Section: Methodsmentioning

confidence: 90%

Detection of an Archaic Clone of Staphylococcus aureus with Low-Level Resistance to Methicillin in a Pediatric Hospital in Portugal and in International Samples: Relics of a Formerly Widely Disseminated Strain?

et al. 1999

View full text Add to dashboard Cite

Close to half of the 878 methicillin-resistant Staphylococcus aureus (MRSA) strains recovered between 1992 and 1997 from the pediatric hospital in Lisbon were bacteria in which antibiotic resistance was limited to β-lactam antibiotics. The other half were multidrug resistant. The coexistence of MRSA with such unequal antibiotic resistance profiles prompted us to use molecular typing techniques for the characterization of the MRSA strains. Fifty-three strains chosen randomly were typed by a combination of genotypic methods. Over 90% of the MRSA strains belonged to two clones: the most frequent one, designated the “pediatric clone,” was reminiscent of historically “early” MRSA: most isolates of this clone were only resistant to β-lactam antimicrobials and remained susceptible to macrolides, quinolones, clindamycin, spectinomycin, and tetracycline. They showed heterogeneous and low-level resistance to methicillin (MIC, 1.5 to 6 μg/ml), carried the ClaI-mecApolymorph II, were free of the transposon Tn554, and showed macrorestriction pattern D (clonal type II::NH::D). The second major clone was the internationally spread and multiresistant “Iberian” MRSA with homogeneous and high-level resistance to methicillin (MIC, >200 μg/ml) and clonal type I::E::A. Surprisingly, the multidrug-resistant and highly epidemic Iberian MRSA did not replace the much less resistant pediatric clone during the 6 years of surveillance. The pediatric clone was also identified among contemporary MRSA isolates from Poland, Argentina, The United States, and Colombia, and the overwhelming majority of these were also associated with pediatric settings. We propose that the pediatric MRSA strain represents a formerly widely spread archaic clone which survived in some epidemiological settings with relatively limited antimicrobial pressure.

show abstract

Section: Methodsmentioning

confidence: 90%

Detection of an Archaic Clone of Staphylococcus aureus with Low-Level Resistance to Methicillin in a Pediatric Hospital in Portugal and in International Samples: Relics of a Formerly Widely Disseminated Strain?

et al. 1999

View full text Add to dashboard Cite

show abstract

“…27 In 1994, a systematic multicenter survey was initiated and since then the prevalence of nosocomial MRSA has been at a high, steady 48-50%. 26,28 The prevalence of MRSA in the community is unknown.…”

Section: Introductionmentioning

confidence: 99%

Low Prevalence of Methicillin-Resistant Strains amongStaphylococcus aureusColonizing Young and Healthy Members of the Community in Portugal

Sá-Leão

Sanches²,

Couto³

et al. 2001

Microbial Drug Resistance

View full text Add to dashboard Cite

Recent reports suggest that methicillin-resistant Staphylococcus aureus (MRSA) may be emerging as a community pathogen. In Portuguese hospitals, the incidence of MRSA among disease causing isolates is extremely high (48-50%). To determine the prevalence of MRSA in the Portuguese community, nasal samples were obtained from 823 draftees, 484 nonmedical university students, and 107 high-school students. In addition, throat samples were obtained from the 823 draftees and S. aureus isolates were also recovered from 283 (13%) nasopharyngeal samples obtained from 2,111 children attending day-care centers. The rate of nasal colonization of S. aureus was 34%, 25%, and 46% for draftees, nonmedical university students, and high-school students, respectively. The rate of pharyngeal colonization of the draftees was 33%. Of the 1,001 S. aureus isolates obtained, seven were MRSA and eight were borderline oxacillin-resistant S. aureus (BORSA). By molecular typing techniques, five of the seven MRSA were identified as belonging to one of three highly epidemic clones, the Brazilian, Iberian, and Pediatric clones of MRSA, which were identified as endemic in Portuguese hospitals. The eight BORSA were all members of clones previously identified in international samples. In spite of the extremely high prevalence of MRSA in Portuguese hospitals, the carriage rate of MRSA in healthy and young individuals remains low.

show abstract

Plasmid analysis of 219 methicillin-resistant Staphylococcus aureus strains with uncommon profiles isolated in Lisbon

Melo‐Cristino

Pereira

1989

Journal of Hospital Infection

View full text Add to dashboard Cite

Infection with methicillin-gentamicin-resistant Staphylococcus aureus strains in a paediatric surgical unit in Lisbon

Cited by 9 publications

References 3 publications

Detection of an Archaic Clone of Staphylococcus aureus with Low-Level Resistance to Methicillin in a Pediatric Hospital in Portugal and in International Samples: Relics of a Formerly Widely Disseminated Strain?

Detection of an Archaic Clone of Staphylococcus aureus with Low-Level Resistance to Methicillin in a Pediatric Hospital in Portugal and in International Samples: Relics of a Formerly Widely Disseminated Strain?

Low Prevalence of Methicillin-Resistant Strains amongStaphylococcus aureusColonizing Young and Healthy Members of the Community in Portugal

Plasmid analysis of 219 methicillin-resistant Staphylococcus aureus strains with uncommon profiles isolated in Lisbon

Contact Info

Product

Resources

About