Infections byLeptospira interrogans, Seoul Virus, andBartonellaspp. Among Norway Rats (Rattus norvegicus) from the Urban Slum Environment in Brazil

Abstract

Norway rats (Rattus norvegicus) are reservoir hosts for zoonotic pathogens that cause significant morbidity and mortality in humans. Studies evaluating the prevalence of zoonotic pathogens in tropical Norway rat populations are rare, and data on co-infection with multiple pathogens are nonexistent. Herein, we describe the prevalence of leptospiral carriage, Seoul virus (SEOV), and Bartonella spp. infection independently, in addition to the rates of co-infection among urban, slum-dwelling Norway rats in Salvador, Brazil, trapped during the rainy season from June to August of 2010. These data were complemented with previously unpublished Leptospira and SEOV prevalence information collected in 1998. Immunofluorescence staining of kidney impressions was used to identify Leptospira interrogans in 2010, whereas isolation was used in 1998, and western blotting was used to detect SEOV antibodies in 2010, whereas enzyme-linked immunosorbent assay (ELISA) was used in 1998: in 2010, Bartonella spp. were isolated from a subsample of rats. The most common pathogen in both years was Leptospira spp. (83%, n = 142 in 1998, 63%, n = 84 in 2010). SEOV was detected in 18% of individuals in both 1998 and 2010 (n = 78 in 1998; n = 73 in 2010), and two species of Bartonella were isolated from 5 of 26 rats (19%) tested in 2010. The prevalence of all agents increased significantly with rat mass/age. Acquisition of Leptospira spp. occurred at a younger mass/age than SEOV and Bartonella spp. infection, suggesting differences in the transmission dynamics of these pathogens. These data indicate that Norway rats in Salvador serve as reservoir hosts for all three of these zoonotic pathogens and that the high prevalence of leptospiral carriage in Salvador rats poses a high degree of risk to human health.

Serosurvey of Leptospira spp. and Toxoplasma gondii in rats captured from two zoos in Southern Brazil

“…The frequency of seropositive rats in both zoos was lower than that previously described in Baltimore (32%) 12 , Copenhagen (94%) 8 , and Salvador (63.1%) 13 . This unexpected outcome might be explained by the limited sample size in this study.…”

“…seropositivity between sexes (p = 0.211) or sampling locations (p = 0.645). Although a higher prevalence in females was found in a previous study 12 , no such difference was expected, as the risk of exposure is similar for both sexes 2,8,13 .…”

“…Nevertheless, the copenhageni and icterohaemorrhagiae serovars, both in the Icterohaemorrhagiae serogroup, have been previously described in rats, which are considered the major reservoirs of Leptospira spp. 13 .…”

Association of Bartonella Species with Wild and Synanthropic Rodents in Different Brazilian Biomes

“…have been conducted in Brazil: one in synanthropic rodents (Rattus norvegicus) in the state of Bahia (northeastern Brazil) and one in wild rodents in the state of Mato Grosso do Sul (midwestern Brazil). These studies reported the first isolation of B. queenslandensis (5/26) and B. tribocorum (1/26) in synanthropic rodents (20) and the first detection of Bartonella vinsonii subsp. arupensis in wild rodents (21).…”

Rat infestation associated with environmental deficiencies in an urban slum community with high risk of leptospirosis transmission

“…A few major cities in Brazil (São Paulo, Salvador, Recife and Curitiba) have shifted the focus of their interventions from rodenticide-focused pest control to a more holistic approach of integrated interventions (e.g., education, poisoning and environmental modifications). Nevertheless, the lack of knowledge regarding the social and ecological determinants influencing rodent infestation in these settings has been a barrier to implement more effective rodent control 13,14,15,16 .…”