Infections with multiple high‐risk HPV types are associated with high‐grade and persistent low‐grade intraepithelial lesions of the cervix

Brot, Louise De; Pellegrini, Bruno; Moretti, Sabrina; Carraro, Dirce Maria; Soares, Fernando Augusto; Baiocchi, Glauco; Cunha, Isabela Werneck da; Andrade, Victor Piana de

doi:10.1002/cncy.21789

Cited by 53 publications

(51 citation statements)

References 19 publications

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“…This observation was corroborated by our findings in that a high viral load of HPV 16 was significantly different in LSIL compared to other groups [38]. Recent studies have shown a decrease in multiple HPV infections in HSIL cases compared to LSIL, unlike our study, where we found 55% multiple HPV infections in LSIL versus 62% in HSIL; in this Brazilian study, they found 58% multiple HPV infections in LSIL and 55% in HSIL cases [39]. Considering that our method is unable to detect LR-HPV, we cannot discard samples that might also have multiple HPV infections with an LR-HPV.…”

Section: Discussionsupporting

confidence: 90%

“…This kit is based on simultaneous real-time amplification (multiplex PCR) of DNA fragments of 14 HR-HPV types (HPV 16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68) and a DNA fragment of the ß-globin gene as an internal endogenous control, carried out in four separated reaction tubes. Each genotype is detected in a separate fluorescent channel (FAM, JOE, ROX, and Cy5) that allows its detection and quantification (a total of 16 qPCR probes are measured).…”

Section: Hpv Genotyping and Viral Loadmentioning

confidence: 99%

“…The most common HR-HPV types in CC (16,18,31,33,35,39,45,51,52,56,58,59 and 68) are associated with low-grade cervical lesions (LSIL), high-grade cervical lesions (HSIL), and CC [10]. HPV types 16 and 18 are responsible for 70% of HPV associated cancer cases worldwide [11].…”

Section: Introductionmentioning

confidence: 99%

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Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Oyervides-Muñoz

Pérez-Maya

Sánchez‐Domínguez

et al. 2020

Viruses

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Persistent high-risk human papillomavirus (HR-HPV) infections play a major role in the development of invasive cervical cancer (CC), and screening for such infections is in many countries the primary method of detecting and preventing CC. HPV typing can be used for triage and risk stratification of women with atypical squamous cells of undetermined significance (ASC-US)/low-grade cervical lesions (LSIL), though the current clinical practice in Mexico is to diagnose CC or its preceding conditions mainly via histology and HR-HPV detection. Additional information regarding these HPV infections, such as viral load and co-infecting agents, might also be useful for diagnosing, predicting, and evaluating the possible consequences of the infection and of its prevention by vaccination. The goal of this follow-up hospital case study was to determine if HPV types, multiple HPV infections, and viral loads were associated with infection persistence and the cervical lesion grade. A total of 294 cervical cytology samples drawn from patients with gynecological alterations were used in this study. HPV types were identified by real-time PCR DNA analysis. A subset of HPV-positive patients was reevaluated to identify persistent infections. We identified HPV types 16, 18, and 39 as the most prevalent. One hundred five of the patients (59%) were infected with more than one type of HPV. The types of HPV associated with multiple HPV infections were 16, 18, and 39. In the follow-up samples, 38% of patients had not cleared the initially detected HPV infection, and these were considered persistent. We found here an association between multiple HPV infections and high viral loads with and infection persistence. Our findings suggest there are benefits in ascertaining viral load and multiple HPV infections status of HR-HPV infections for predicting the risk of persistence, a requirement for developing CC. These findings contribute to our understanding of HPV epidemiology and may allow screening programs to better assess the cancer-developing risks associated with individual HR-HPV infections.

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Section: Discussionsupporting

confidence: 90%

Section: Hpv Genotyping and Viral Loadmentioning

confidence: 99%

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Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Oyervides-Muñoz

Pérez-Maya

Sánchez‐Domínguez

et al. 2020

Viruses

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“…When L1 gene methylation unchanged, it was 4.409 times to appear DNA heteroploid in multiple infection than single infection in Han women, in Uygur women, the OR was 3.279, the results accord with previous studies. [15,26] Compared to the Uygur women, HPV-16 infection status seemed to be more influence on Han women (OR: 3.297 vs 4.409). And L1 gene methylation created larger influence on Uygur women than Han women, in the same HPV infection, with a mild degree of L1 gene methylation as reference, Uygur women increase 5.075 and 63.634 times to appear DNA heteroploid when L1 gene methylation changed to moderate and severe grade, but the 2 OR values were 3.313 and 20.592 in Han women, which was smaller than Uygur women.…”

Section: Discussionmentioning

confidence: 99%

Increased HPV L1 gene methylation and multiple infection status lead to the difference of cervical epithelial cell lesion in different ethnic women of Xinjiang, China

et al. 2017

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Human papillomavirus (HPV) L1 gene methylation deeply involved in the progression and heterogeneity of cervical cell epithelial lesions. The DNA ploidy also represented the early lesions of cervical cell, and it was associated with different HPV infection status in different ethnic women. So, the research was to explore whether it was possible that HPV L1 gene methylation and HPV infection status as the risk factors to lead to the differences of cervical epithelial cells’ lesions in different ethnics women.The flow-through hybridization and gene chip for HPV genotypes test, general characteristics, and cervical exfoliated cell samples were collected from 94 Uygur and 79 Han women with HPV-16 infection. The cases were divided into the single HPV-16 (sHPV-16) infection group and multiple HPV-16 (mHPV-16) infection group in each ethnic women. The DNA ploidy was analyzed by flow cytometry, and the methylation-sensitive high resolution melting (MS-HRM) was used to test the HPV-16 L1 gene methylation, the results of methylation was segmented into mild methylation, moderate methylation, and severe methylation groups. Multifactor logistic analysis explored the relation between DNA heteroploid and HPV-16 infection status, HPV-16 L1 gene methylation in different ethnic women.The higher proportion of mHPV-16 infection in Uygur than Han women (61.7% vs 38.0%). L1 gene methylation had statistic difference between single and mHPV-16 infection under the same ethnic women. The proportion of DNA heteroploid had statistic difference between different HPV-16 infection status or different L1 gene methylation grades in Han or Uygur women. Both L1 gene methylation and HPV infection status were the risk factors of DNA heteroploid. Compared to the sHPV-16 infection, the odds ratio (OR) of mHPV-16 infection were 4.409 (CI: 1.398–13.910) and 3.279 (CI: 1.069–10.060) in Han and Uygur women. Compared the mild L1 gene methylation, the OR of moderate L1 gene methylation were 3.313 (CI: 1.002–10.952) and 5.075 (CI: 1.385–18.603) in Han and Uygur women, the OR of severe L1 gene methylation were 20.592 (CI: 3.691–114.880) and 63.634 (CI: 10.400–389.368) in Han and Uygur women.The study first reported that HPV L1 gene methylation and HPV infection status were the risk factors to the DNA heteroploid of cervical cell in different ethnics women, HPV L1 gene methylation and infection status should be recommended to the existing system of cervical lesion screening in order to provide better serves for the HPV infected women, especially for the ethnic women with high proportion of severe L1 gene methylation and multiple infection status.

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“…Graças às técnicas da biologia molecular, estabeleceu-se a relação entre a infecção persistente pelo HPV com alguns tipos virais e o câncer cervical e que infectam preferencialmente os órgãos genitais (vulva, vagina, colo uterino, pênis e áreas perianais) e orofaringe. Dessa forma, o HPV mesmo sendo reconhecido como fator de risco fundamental para câncer cervical não pode ser visto como a causa única para o desenvolvimento deste tipo de neoplasia maligna 13 .…”

Section: Revisão De Literaturaunclassified

Fatores de risco de mulheres adolescentes e jovens frente ao Papilomavírus Humano [Human Papilloma Virus-related risk factors for adolescent and young women] [Factores de riesgo para las adolescentes y jóvenes mujeres ante el Virus del Papiloma Humano]

et al. 2017

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RESUMOObjetivo: identificar os fatores de risco à infecção pelo Papilomavírus Humano (HPV) associados aos comportamentos e atitudes de adolescentes e jovens de uma unidade escolar de Ensino Médio do Rio de Janeiro. Método: estudo quantitativo, descritivo, realizado de maio a novembro de 2012, com 128 mulheres entre 15 e 24 anos. Os dados foram coletados por questionário e receberam tratamento estatístico descritivo, sendo destacados em variáveis demográficas, econômicas e comportamentais. O estudo foi aprovado pelo protocolo n o 030/2011. Resultados: os dados apontaram haver resistência ao uso de preservativo nas relações sexuais, sendo que 81,3% das mulheres nunca utilizaram camisinha nas relações. O estudo também destacou como uma das características entre as participantes o uso de preservativo somente na primeira relação. O preservativo feminino não foi utilizado pelo grupo estudado. Conclusão: o estudo mostra dados epidemiológicos e questões socioculturais importantes que indicam a necessidade de investigações aprofundadas, além da atuação do enfermeiro em novas estratégias educativas visando à efetividade na mudança de comportamento e atitude desses segmentos populacionais. Palavras-chave: Saúde da mulher; Papilomavírus Humano; fatores de risco; preservativos.ABSTRACT Objective: to identify risk factors for human papillomavirus (HPV) infection associated with behaviors and attitudes of adolescents and young people at an upper secondary school in Rio de Janeiro. Method: in this quantitative, descriptive study conducted from May to November 2012, data were collected by questionnaire from 128 women from 15 to 24 years old, and received descriptive statistical treatment, which highlighted demographic, economic and behavioral variables. The study was approved by the research ethics committee of the Anna Nery School of Nursing, São Francisco de Assis School Hospital, Rio de Janeiro Federal University (protocol No. 030/2011). Results: the data indicated resistance to condom use in sexual relations: 81.3% of the women never used condoms in their relationships. Another of the participants' characteristics highlighted by the study was the use of a condom in the first relation only. Female condoms were not used by the group studied. Conclusion: the study shows epidemiological data and important sociocultural issues that indicate the need for in-depth research, as well as the nurses' playing a role in new educational strategies designed to be effective in changing behavior and attitudes in these population segments.

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Infections with multiple high‐risk HPV types are associated with high‐grade and persistent low‐grade intraepithelial lesions of the cervix

Cited by 53 publications

References 19 publications

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Increased HPV L1 gene methylation and multiple infection status lead to the difference of cervical epithelial cell lesion in different ethnic women of Xinjiang, China

Fatores de risco de mulheres adolescentes e jovens frente ao Papilomavírus Humano [Human Papilloma Virus-related risk factors for adolescent and young women] [Factores de riesgo para las adolescentes y jóvenes mujeres ante el Virus del Papiloma Humano]

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