Infectious and Non-infectious Mutants of Cauliflower Mosaic Virus DNA

Melcher, Ulrich; Steffens, David L.; Lyttle, David J.; Lebeurier, G.; Lin, Haifan; Choe, In Seong; Essenberg, Richard C.

doi:10.1099/0022-1317-67-7-1491

Cited by 16 publications

(13 citation statements)

References 24 publications

(41 reference statements)

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“…A chlorotic lesion was typically found to yield 5 to 10ng of CaMV DNA, confirming and extending previous observations (Melcher et al, 1981(Melcher et al, , 1986. In contrast, no CaMV signal was detectable in the green area of a leaf.…”

Section: Discussionsupporting

confidence: 78%

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The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

Riederer

Grimsley²,

Höhn³

et al. 1992

Journal of General Virology

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We inoculated the leaves of turnip plants (Brassica campestris spp. rapa cv. Just Right) with two cauliflower mosaic viruses (CaMVs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. Surprisingly, analysis of viral DNA in single primary chlorotic lesions revealed the presence of both mutants. In contrast, the secondary chlorotic lesions and systemically infected leaves contained virus molecules of either one or the other type only. Infection of plants with different ratios of the two reporter viruses showed that this ratio is not conserved during systemic virus spread. Infection with CaMV DNA in the form of heteroduplexes containing a single mismatched base pair, in which each strand carried a distinct diagnostic marker, provided us with evidence that the mismatch was subjected to a repair process in the host plant.

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Section: Discussionsupporting

confidence: 78%

“…Primary chlorotic lesions have been reported to contain viral DNA detected by a leaf blotting technique (Melcher et al, 1981), and the amount of viral DNA per unit area of leaf has been estimated (Melcher et al, 1986). However, it is difficult to quantify the amount of DNA present in single lesions by these techniques.…”

Section: Introductionmentioning

confidence: 99%

The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

Riederer

Grimsley²,

Höhn³

et al. 1992

Journal of General Virology

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“…The 30K superfamily phylogenetic tree places all tubule-forming MPs in a separate cluster (15), suggesting an important relationship between MP secondary structure and movement strategy. Our results and previous results (19) support this prediction, showing that all tested 30K family members encoded by an RNA virus mediate virus NPC movement, whereas only a subset have developed the additional feature of controlling tubule-guided transport.…”

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confidence: 99%

Caulimoviridae Tubule-Guided Transport Is Dictated by Movement Protein Properties

Sánchez‐Navarro

Fajardo

Zicca

et al. 2010

J Virol

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Plant viruses move through plasmodesmata (PD) either as nucleoprotein complexes (NPCs) or as tubuleguided encapsidated particles with the help of movement proteins (MPs). To explore how and why MPs specialize in one mechanism or the other, we tested the exchangeability of MPs encoded by DNA and RNA virus genomes by means of an engineered alfalfa mosaic virus (AMV) system. We show that Caulimoviridae (DNA genome virus) MPs are competent for RNA virus particle transport but are unable to mediate NPC movement, and we discuss this restriction in terms of the evolution of DNA virus MPs as a means of mediating DNA viral genome entry into the RNA-trafficking PD pathway.

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“…DNAs of the Cabb S, NY8153, W and CM4-184 CaMV isolates were obtained from plasmids pCa37 (Lebeurier et al, 1982), pCMS31 (Armour et al, 1983), pLW303X (Walden & Howell, 1982;Choe et aL, 1985) and pLW414 (Howell et al, 1980), respectively, pCaDH was constructed as described by Armour et aL (!983) from DNA extracted from virions obtained from turnip plants infected with the D/H isolate of CaMV (Balazs et al, 1982). 'Fwo derivatives of pCa37, each bearing a small alteration in open reading frame (ORF) III, were used: pUMI30 (Melcher et al, 1986b) and pXZG ( Fig. 1) (Choe et al, 1985).…”

Section: Methodsmentioning

confidence: 99%

“…The recombinant pVR246 ( Fig. 1) was similarly obtained from inplanta recombination (V. Vaden & U. Melcher, unpublished data) between DNA of the Cabb S mutant pUM24 (Melcher et al, 1986b) and pLW76 (Howell et al, 1981) which contained mutated DNA of the CM4-184 isolate (Howarth et al, 1981). Plasmids pCS/W and pW/CS (Fig.…”

Section: Methodsmentioning

confidence: 99%

Competition between Isolates and Variants of Cauliflower Mosaic Virus in Infected Turnip Plants

Zhang¹,

Melcher²

1989

Journal of General Virology

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SUMMARYThe Cabb S isolate of cauliflower mosaic virus (CaMV) is the only isolate that can be recovered from turnip plants mixedly infected with Cabb S and an infectious variant of Cabb S. Analysis of the progeny resulting from mixed infections of turnip plants was done for a better understanding of this dominance. Cabb S DNA was the dominant DNA recovered from plants after a mixed infection with Cabb S and D/H or W isolates of CaMV, whereas UM130 (derived from Cabb S by the insertion of a short oligonucleotide in open reading frame III) coexisted in turnip plants with D/H, NY8153, CM4-184 and W isolates. To determine whether there are additional sequences responsible for the ability of Cabb S to dominate over UM130, coinoculation experiments were done using chimeric DNAs, obtained by in vivo recombination (IC141, IC143 and VR246) and by in vitro construction (W/CS and CS/W). Chimeras IC141, IC143 and VR246 coexisted in turnip plants with UM130. Chimera CS/W dominated over UM 130, whereas UM 130 dominated over W/CS. The location of Cabb S sequences in these chimeras suggests that more than one region is responsible for the competitive advantage of Cabb S DNA. A different modification of Cabb S DNA in open reading frame III also destroyed the ability of the DNA to dominate over the W isolate. In DNA from virions obtained after co-infection with U M 130 and CS/W, more molecules were recovered with U M 130-specific sequences at position 1364 than with those at position 2040. This and similar results with progeny from W/CS and UM130 co-infection suggest that genetic exchanges had occurred. However, exchanges were not detected in progeny from other mixed infections. Thus the dominance of the Cabb S isolate in mixed infections was due primarily to a better competitive ability of the Cabb S DNA. Plants inoculated first with UM130 and 2 to 8 days later with Cabb S contained only UM130 CaMV DNA, indicating that dominance was unable to overcome cross-protection.

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Infectious and Non-infectious Mutants of Cauliflower Mosaic Virus DNA

Cited by 16 publications

References 24 publications

The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

Caulimoviridae Tubule-Guided Transport Is Dictated by Movement Protein Properties

Competition between Isolates and Variants of Cauliflower Mosaic Virus in Infected Turnip Plants

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