Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA

O’Rear, Julian J.; Mizutani, Satoshi; Hoffman, Guy G.; Fiandt, M.; Temin, Howard M.

doi:10.1016/0092-8674(80)90628-5

Cited by 60 publications

(45 citation statements)

References 20 publications

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“…To accomplish these aims, DNA from uninfected chicken cells was digested with EcoRI, electrophoresed through a 0.5~ agarose gel, transferred to nitrocellulose, and hybridized to a pool of 10 cloned SNV proviruses which had been nick-translated. These proviruses and adjoining cellular DNA had previously been isolated as EcoRI fragments from SNV-infected chicken cells (O'Rear et al, 1980). Because EcoRI does not cleave within the SNV genome, whole proviruses and adjacent cellular sequences are obtained from EcoRI-digested 'preintegration' sites for these proviruses is predicted by the sum of the lengths of the cellular DNA 5' and 3' to the proviruses.…”

Section: Characteristics Of Cellular Dna Surrounding Integration Sitesmentioning

confidence: 99%

“…A library of SNV-infected rat cell DNA in 2 Charon 4A was constructed from NRK cells chronically infected with SNV (Keshet & Temin,1~79) as previously described for SNV-infected chicken cells (O'Rear et al, 1980). The single SNV provirus recovered from the library is infectious in DNA transfection assays in chicken cells.…”

Section: Cells Viruses and Dnamentioning

confidence: 99%

“…The cells used for this purpose were derived from the same embryo used for infection and subsequent isolation of SNV proviruses described by O'Rear et al (1980).…”

Section: Cells Viruses and Dnamentioning

confidence: 99%

“…A 2 Charon 4A clone containing sequences homologous to chicken DNA adjacent to the SNV provirus in clone 60 (O'Rear et al, 1980) was obtained by screening the chicken DNA recombinant library constructed by Dodgson et al (1979). Subcloned DNAs of 5' and 3" cellular sequences adjacent to SNV proviruses 60, 70, 3-73, 44, 32, and 4 (O'Rear et al, 1980; were mixed together, nick-translated, and used as a hybridization probe in screening.…”

Section: Cells Viruses and Dnamentioning

confidence: 99%

“…When DNA from chicken cells infected with SNV is digested with EcoRI and the resulting fragments are separated by electrophoresis into agarose gels, integrated SNV DNA is found associated with a wide size range of fragments (Keshet & Temin, 1978. The cell-virus junctions of seven SNV proviruses from infected chicken cells have been cloned and sequenced previously (O'Rear et al, 1980;. The length of the cellular repeat sequence for each provirus is five nucleotides, but the sequence repeated is different in each case.…”

Section: Introductionmentioning

confidence: 99%

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Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Fitts

Temin

1983

Journal of General Virology

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267 SUMMARYThe size of the direct repeats of cellular DNA next to a spleen necrosis virus (SNV) provirus from infected rat cells and the nature of the cellular DNA surrounding SNV integration sites in chicken DNA were studied. A five-base pair repeat, ATTTT, was observed at the SNV-rat cell junctions. Five of ten SNV proviruses from chicken cells were flanked by unique DNA and five others were flanked by repetitive DNA. No large rearrangements of cellular DNA at SNV integration sites were observed. A clone of uninfected chicken DNA containing the presumptive unoccupied integration site for one SNV provirus was obtained and part of it was sequenced. A small substitution, approximately 60 nucleotides, was observed at the site of virus DNA insertion. Its significance is not known.

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Section: Characteristics Of Cellular Dna Surrounding Integration Sitesmentioning

confidence: 99%

Section: Cells Viruses and Dnamentioning

confidence: 99%

“…The cells used for this purpose were derived from the same embryo used for infection and subsequent isolation of SNV proviruses described by O'Rear et al (1980).…”

Section: Cells Viruses and Dnamentioning

confidence: 99%

Section: Cells Viruses and Dnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Fitts

Temin

1983

Journal of General Virology

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Cloned single- and double-stranded DNA copies of potato spindle tuber viroid (PSTV) RNA and co-inoculated subgenomic DNA fragments are infectious.

Tabler¹,

Sänger²

1984

The EMBO Journal

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A set of monomeric and oligomeric potato spindle tuber viroid (PSTV) specific DNA forms representing complete DNA copies of the circular PSTV RNA genome were constructed and cloned in plasmid pBR322 and bacteriophage M13. Both single‐ and double‐stranded PSTV DNAs are capable of initiating viroid replication in mechanically inoculated tomato plants where it normally proceeds via the RNA‐RNA pathway without DNA being involved. All dimeric and higher multimeric forms were infectious irrespective of their polarity in the case of single‐stranded DNA and regardless of their orientation in the vector DNA in the case of double‐stranded DNA. The vector‐inserted monomeric PSTV DNA units were also found to be infectious but of low specific infectivity which was increased when these monomers had been excised. Even two subgenomic DNA fragments, representing together the 359 nucleotides of the PSTV RNA genome, initiated the synthesis of viroid RNA progeny when co‐inoculated although each fragment by itself is non‐infectious. These results are discussed with respect to the infectivity previously observed with certain cloned DNAs of conventional RNA and DNA viruses.

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Developments in Molecular Virology: Cloning of Retrovirus DNA in Bacteria and Cloning of other DNA in Retroviruses

Temin¹

1985

Recombinant DNA Research and Viruses

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Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA

Cited by 60 publications

References 20 publications

Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Cloned single- and double-stranded DNA copies of potato spindle tuber viroid (PSTV) RNA and co-inoculated subgenomic DNA fragments are infectious.

Developments in Molecular Virology: Cloning of Retrovirus DNA in Bacteria and Cloning of other DNA in Retroviruses

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