2023
DOI: 10.1002/biot.202300254
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Infectious clone of a contemporary Tembusu virus and replicons expressing reporter genes or heterologous antigens from poultry viruses

Jerome D. G. Comes,
Meliawati Poniman,
Linda van Oosten
et al.

Abstract: The novel mosquito‐borne Tembusu virus (TMUV, family Flaviviridae) was discovered as the cause of a severe outbreak of egg‐drop syndrome affecting ducks in Southeast Asia in 2010. TMUV infection can also lead to high mortality in various additional avian species such as geese, pigeons, and chickens. This study describes the construction of an infectious cDNA clone of a contemporary duck‐isolate (TMUV WU2016). The virus recovered after transfection of BHK‐21 cells shows enhanced virus replication compared to th… Show more

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