Infectious Herpesvirus DNA

Graham, Frank L.; Veldhuisen, G.; Wilkie, N. M.

doi:10.1038/newbio245265a0

Cited by 77 publications

(38 citation statements)

References 7 publications

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“…This work claimed that the "calcium technique" was a suitable system to study transformation by adenovirus DNA and the efficiency of transformation, though not high, appeared to be reasonably reproducible (Graham & Van Der EB, 1973b). In another study, Graham, Veldhuisen and Wilkie used the aforementioned technique to investigate the infectivity of herpes simplex virus type I (HSV-I) (Graham et al, 1973). In 1975, Abrahams and Van Der EB made a transformation of rat kidney cells and mouse 3T3 cells by DNA from Simian Virus 40 using "calcium technique".…”

Section: Historical Viewmentioning

confidence: 99%

Nano-Particulate Calcium Phosphate as a Gene Delivery System

Mostaghaci¹,

Hanifi²,

Loretz³

et al. 2011

Non-Viral Gene Therapy

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Section: Historical Viewmentioning

confidence: 99%

Nano-Particulate Calcium Phosphate as a Gene Delivery System

Mostaghaci¹,

Hanifi²,

Loretz³

et al. 2011

Non-Viral Gene Therapy

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“…Both total intracellular DNA prepared from infected cells (Graham et al, 1973) and purified virus DNA were analysed for their infectivity by the calcium phosphate technique essentially as described by Graham et al (1973), but the cells were transfected in suspension. Specifically, BSC-1 cells, at 5 to 7 days after subcultivation, were trypsinized and suspended in HEPES buffer.…”

Section: Cellsmentioning

confidence: 99%

Low Infectivity of HSV-1 DNA Caused by Defective-interfering Genomes

Hennes-Stegmann

Schröder

1982

Journal of General Virology

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SUMMARYThe infectivity of herpes simplex virus, type 1, strain ANG progeny DNA from standard virus infections and of progeny DNA from infections involving defectiveinterfering virus particles (DI DNA) was compared in transfection assays. No difference in infectivity of virus DNA isolated either from infected cells or from progeny virus was found for a given type of infection. However, the values for the two types of infection differed markedly, with DI progeny DNA being less infectious by more than 2 log10. The low infectivity was mainly due to the presence of interfering DNA molecules in DI progeny DNA, regardless of whether intracellular DNA or DNA extracted from mature virions was analysed. The interfering capacity of DI progeny DNA did not depend on the integrity of the genomes. The physical proximity provided by simultaneous precipitation of infectious and of interfering DNA is an important factor influencing the degree to which DI DNA interferes. Interference by DI DNA in the transfection assay can be partly reversed by the addition of XbaI fragments of standard DNA; in control experiments this fragmented DNA was shown to lead to a reduction rather than to an enhancement of the infectivity of standard virus DNA.

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“…Growing cells will be likely to modify whereas nongrowing cells appear to be more equipped to cleave. Successful use of naked herpes virus DNA [22] or adenovirus DNA [23] as infectious agents appears to have involved the use of growing monolayer cell cultures, however there have been no definitive experiments to answer this problem. Clearly this will be of relevance in any recombinant DNA techniques involving mammalian cells.…”

Section: Alsomentioning

confidence: 99%