Infectious mononucleosis: detection of herpeslike virus and reticular aggregates of small cytoplasmic particles in continuous lymphoid cell lines derived from peripheral blood.

Moses, Harold L.; Glade, Philip R.; Kasel, Julius A.; Rosenthal, Alan S.; Hirshaut, Yashar; Chessin, Lawrence N.

doi:10.1073/pnas.60.2.489

Cited by 69 publications

(31 citation statements)

References 15 publications

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“…All white cell lines showed similar ultrastructural features and could not be distinguished qualitatively either from each other or from cells of previously described lymphoid cell lines [2,13,26,28]. A spectrum of lymphoreticular cells was observed in each culture sample, although the predominant cell would be described as "blast-like" [13] with sparse granular endoplasmic reticulum, prominent Golgi zones, and moderate numbers of mitochondria.…”

Section: Resultsmentioning

confidence: 95%

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Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

et al. 1971

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Continuous leukocyte cultures were established from the blood of three siblings with classical nephropathic cystinosis and from their mother. The cells from patients with cystinosis (CC) contained at least 15-to 20-fold more cystine than cultured leukocytes from normal individuals (NC). The transport of cystine-35 S into CC was similar to uptake into NC when tested at pH 5.0 and pH 7.4.The cultured CC could not be distinguished by ultrastructure from NC Crystalline inclusions which could easily have been mistaken for cystine were observed in the lysosome of a normal cell. The modal number of chromosomes in CC and NC lines was 46. There was a marked degree of aneuploidy. Tetraploidy was present in 3.8-9.6% of metaphases. Structural rearrangements and breaks were seen in all cell lines. Speculation

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Section: Resultsmentioning

confidence: 95%

“…Dense microtubular structures (Fig. 3) within the endoplasmic reticulum, previously described in lymphoid cell lines of neoplastic and nonneoplastic origin [3,6,12,26,28], were abundant in cells of two patients with cystinosis and in one of the controls. Enlarged mitochondria with paracrystalline inclusions (Fig.…”

Section: Resultsmentioning

confidence: 97%

Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

et al. 1971

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“…Since these structures have not been detected in hepatocytes of chimpanzees experimentally infected with hepatitis A or hepatitis B virus, it is considered that cytoplasmic tubular structures serve as useful morphological markers in chimpanzees successfully infected with NANB hepatitis agents. Similar structures, however, have been observed in a variety of cells; numerous types of malignant turnours [Uzman et al, 19711, cultured cell lines [Chandra et al, 19691, lymphocytes of patients with acquired immune deficiency syndrome (AIDS) [Sidhu et al, 19831, leukaemia cells of Japanese patients with adult T cell leukaemia [Shamoto et al, 19811, and cells infected with other RNA and DNA viruses [Moses et al, 1968;Smith et al, 19681. Furthermore, Kamimura et a1 [ 19831 demonstrated identical ultrastructural changes in hepatocytes of HBsAg carrier chimpanzees experimentally infected with delta agent.…”

Section: Discussionmentioning

confidence: 94%

Studies on transmission of human non‐A, non‐B hepatitis to marmosets

Watanabe¹,

Katagiri²,

Kojirna³

et al. 1987

Journal of Medical Virology

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Two sera obtained from four healthy blood donors, which caused non-A, non-B post-transfusion hepatitis in two recipients, were experimentally inoculated into nine marmosets. Three of seven marmosets developed acute hepatitis characterized by the elevation of serum concentrations of glutamic pyruvic transaminase (GPT) and/or isocitric dehydrogenase (ICD) 8-11 weeks after inoculation. Four of seven showed histopathological changes of acute hepatitis in liver biopsy specimens during the biochemically acute phase. In electron microscopic examination, attached membrane-like structures, which consisted of two-unit membranes of two neighboring endoplasmic reticula with electron-dense material between them, were noted in cytoplasm of hepatocytes during the acute phase of hepatitis. Furthermore, acute-phase sera obtained from two animals were inoculated into four additional marmosets, and non-A, non-B hepatitis was successfully passaged in two of them. The results of this study indicate that certain species of marmoset monkeys are susceptible to human non-A, non-B hepatitis agents and provide a useful animal model for non-A, non-B hepatitis.

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“…Included are patients with infectious mononucleosis (Moses et al, 1968;Payne et al, 19821, after infection with Coxsackie B [Haas and Yunis, 19701, herpes simplex [Baringer,197 11,rubella virus [Kistler,197.51, accompanying collagen diseases, especially systemic lupur erythematodes [Gyorkey et al, 19721. in some malignant tumors [Gyorkey et al, 19711.…”

Section: Discuss I 0 Nmentioning

confidence: 99%

Ultrastructural alterations in hepatocytes and sinus endothelia in experimental non‐A, non‐B hepatitis in chimpanzees with and without immunoglobulin prophylaxis

Spichtin

Eder²,

Gudat

et al. 1983

Journal of Medical Virology

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Three chimpanzees were inoculated with an infectious factor VIII preparation. Two of the chimpanzees received in addition a human immunoglobulin preparation as used for prophylaxis in humans. All three chimpanzees developed an acute limited non-A, non-B hepatitis as judged from light and electron microscopic markers after an incubation period of two weeks. The use of immunoglobulin did not prevent the infection. A prolonged incubation of 15 weeks, however, was observed in one animal when alanine aminotransferase (ALT) elevation was used as criterion of infection. In the electron microscope, non-A, non-B hepatitis was characterized by tubular structures, spongelike inclusions and attaching curved membranes, in the absence of nuclear viruslike particles. An additional finding were viruslike particles in crystalline arrays which were found in the cytoplasm of sinusoidal-lining endothelial cells and tubuloreticular complexes.

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Infectious mononucleosis: detection of herpeslike virus and reticular aggregates of small cytoplasmic particles in continuous lymphoid cell lines derived from peripheral blood.

Cited by 69 publications

References 15 publications

Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

Studies on transmission of human non‐A, non‐B hepatitis to marmosets

Ultrastructural alterations in hepatocytes and sinus endothelia in experimental non‐A, non‐B hepatitis in chimpanzees with and without immunoglobulin prophylaxis

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