Infectious nucleic acid in plants with tobacco mosaic

Studies on Viral Pathogenesis in Plant Hosts.

“…Our results would fit with the view of FRY and MATTHKWS. Even in whole leaf tissues of tobacco plants infected with TMV, infectious RNA precursor for complete virus has been detected during early stages of infection (COCHRAN and CHIDESTER 1957, HAMERS-CASTERMAN and JEENER 1957, ENGLER and SCHRAMM 1959, 1960, WHITFELD, DAY, HELMS and VENABLES 1960, DIENER 1962, VAN KAMMEN 1963, S.\RKAR 1965. However, these data reported by several workers did not involve in the RNA precursor as a cell-to-cell transport form.…”

Physiology of Virus Diseases

“…Leaf extracts contain ribonuclease and other sub stances that readily inactivate virus nucleic acid, whereas the leaf proteases do not readily hydrolyse virus proteins. As the proteins are also readily identified serologically, it is not surprising that these rather than free virus nucleic acid have been most often reported; however, by grinding at low temperatures (liquid nitrogen) leaves in which TMV was increasing rap· idly, extracts have been made that are stated to contain infective nucleic acid (26).…”