Infectious Titres of Human Papillomaviruses (HPVs) in Patient Lesions, Methodological Considerations in Evaluating HPV Infectivity and Implications for the Efficacy of High-Level Disinfectants

Dynamics of papillomavirus in vivo disease formation & susceptibility to high-level disinfection—Implications for transmission in clinical settings

“…6 a and 7 ). Taking these results and the work presented in the companion manuscript by the lab of Dr Michelle Ozbun together (https://doi.org/10.1016/j.ebiom.2020.103165) [34] , it is clear that the proper evaluation of virus infectivity and the efficacy of disinfectants requires a clear understanding of the characteristics of the infection models (in vitro and in vivo) , and the source of virus (cell-free virus, virus with exfoliating cells and/or clinically sourced virus), along with the appropriate use of validated controls (e.g. for virus titre, contact time, concertation of disinfectant and the choice of neutralisers).…”

New approaches for infective HPV detection, quantification and inactivation: Preventing accidental virus transmission in medical settings

“…In two articles of EBioMedicine , the authors presented a very detailed and controlled set of experiments to determine the efficacy of ortho-phthalaldehyde (OPA), hypoclorite and hydrogen peroxide in inactivating PVs particles from experimental models, including quasivirions, xenografts, raft cultures and Mus musculus PV type 1 (MmuPV1) induced-lesions in mice. Besides, they included the analysis of HPVs virions retrieved from recurrent respiratory papillomas, anal warts and cervical lesions from patients with hrHPV [ 5 , 6 ].…”

“…The study by Ozbun and colleagues described a precise workflow to determine the efficacy of disinfectants against HPV virions that may remain on surfaces [5] . This is important to prevent the transmission of different HPV types by fomites in nosocomial/health care settings.…”