Infeksi Ganda Begomovirus Dan Crinivirus  Pada Tanaman Tomat Di Kabupaten Magelang , Jawa Tengah  (Double Infections of Begomovirus and Crinivirus  on Tomato Plants at Magelang Regency, Central Java)

Kusumaningrum, Fitri Ayu; Hartono, Slamet; Sulandari, Sri; Somowiyarjo, Susamto

doi:10.22146/jpti.17542

Cited by 9 publications

(11 citation statements)

References 5 publications

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“…However, the sequencing of the full DNA-A of Begomovirus needs to be carried out to achieve precise and accurate identification results (Hernandez-Zepeda et al, 2007). Previous studies also used this method for the quick identification of Begomovirus in melon (Subiastuti et al, 2019), tobacco (Widarta et al, 2017), tomato (Kusumaningrum et al, 2015), bitter melon (Tiwari et al, 2010), and mung bean (M. N. Islam et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The application of polymerase chain reaction (PCR) techniques to detect viral infections in plants is a sensitive, reliable, reproducible, and effective method, especially for large samples (Lopez et al, 2008). Previous studies reported the use of molecular methods to detect Begomovirus infections in chilies and eggplants (Maruthi et al, 2007), okra (Venkataravanappa et al, 2018), and tomatoes (Kusumaningrum et al, 2015). The present study was conducted to determine the effect of using screen houses in chili (Capsicum frutescens L. 'Cempluk') cultivation on the severity of Begomovirus infection and the diversity of Begomovirus coat proteins that cause yellow leaf curling in chilies using molecular methods.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Screen House on Disease Severity and Coat Protein Diversity of Begomovirus-infected Capsicum frutescens L. ‘Cempluk’ from Indonesia

Subiastuti¹,

Putri²,

Permadani³

et al. 2021

JTAS

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Chili can be infected by Begomovirus through whiteflies (Bemisia tabaci) serving as a vector insect. Begomovirus infection causes dwarf plants and yellow curly leaves. The molecular detection of Begomovirus coat protein gene may serve as a preliminary identification of Begomovirus. This study was conducted to observe the differences in the symptom severity of Begomovirus infection in chilies (Capsicum frutescens L. ‘Cempluk’) planted inside and outside a screen house. This study also observed whether or not using a screen house in chili farming affects the diversity of the coat protein of Begomovirus. Symptom observation and sampling were conducted in Madurejo, Prambanan, Sleman. Molecular detection was performed by amplifying the coat protein (CP) gene using the universal primer Krusty and Homer. Results showed 7 plant samples with DNA bands ± 550 bp and confirmed that the plants were positively infected with Begomovirus. The amplified bands were purified and sequenced. The nucleotide sequences were analyzed using BLASTn, followed by phylogenetic analysis using MEGA. Planting chili in the screen house resulted in low disease severity and good crop conditions. The coat protein sequence showed different strains of Begomovirus infected the chili plants inside and outside the screen house. Pepper yellow leaf curl Indonesia virus (PepYLCIV) was found inside the screen house while PepYLCIV [Ageratum] was dominant outside the screen house. Both strains are closely related to other Pepper yellow leaf curl virus (PepYLCV) from various regions in Indonesia. Optical manipulation using an ultraviolet screen or screen house was effective in reducing Begomovirus infection and improving plant performance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Screen House on Disease Severity and Coat Protein Diversity of Begomovirus-infected Capsicum frutescens L. ‘Cempluk’ from Indonesia

Subiastuti¹,

Putri²,

Permadani³

et al. 2021

JTAS

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“…Sampling was conducted in 2018. Ketep was chosen as a sampling site because it is one of the horticulture produc tion areas in Central Java and reported as endemic area of begomovirus infections, as reported by Kusumaningrum et al (2017). Symptom appearance of the sample was mo saic, leaf yellowing, stunting, which are common symp toms of begomovirus infection (Figure 1).…”

Section: Sampling Locationmentioning

confidence: 99%

“…Diseases caused by begomovirus in fection initially only occurred in the lowlands. But in re cent years, the disease is also found in the highlands area (Kusumaningrum et al 2017). Besides, diseases caused by begomovirus have also been reported affecting new hosts, including eggplant and luffa (Kintasari et al; Giovanni et al 2020).…”

Section: Introductionmentioning

confidence: 99%

A recombinant DNA‐satellite associated with Pepper yellow leaf curl Indonesia virus in highland area

Kandito

Hartono

Sulandari

et al. 2021

Indones. J. Biotechnol.

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Yellow curl disease caused by begomovirus is a major threat for horticulture in Indonesia. Control mea‐ sures for the disease face several constraints, one of which is the association between begomovirus and DNA satellites which can affect the severity of symptoms. In this study, we detected the presence of a DNA satellite associated with begomovirus in a highland area. The sample was obtained from Ketep, Magelang, located approximately 1400 meters above sea level. Begomovirus was detected using primers PAL1V1978/PAR1C715 that resulted in an amplicon of ap‐ proximately 1600bp. The presence of this satellite was detected using primers CLB36F/CLB37R, resulting in full‐length satellite genome of approximately 1300bp. Sequence analysis showed the sample was infected by Pepper yellow leaf curl Indonesia virus (PepYLCIV) and a non‐coding satellite which resembled some characteristics of common betasatellites with imperfect putative ORF βC1. SimPlot analysis revealed the recombination event between betasatellites and DNA‐B of PepYLCIV. The satellite found in this study is thought to be the result of recombination due to multiple infections in plants.

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“…Secara umum, peningkatan keparahan penyakit akan terjadi apabila adanya koinfeksi dari satu atau dua virus dibandingkan apabila diinfeksi oleh satu virus (Choi et al, 2002;Kusumaningrum et al, 2015;Syller, 2012). Akan tetapi, infeksi ganda dapat menimbulkan tekanan pada salah satu virus (Dufresne et al, 1999: Poolpol andInouye 1986;Syller dan Grupa, 2016).…”

Section: Pembahasanunclassified

Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai

Trisno

Jamsari²,

Hidayat³

2021

J. Proteksi. Tanaman

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Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.

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Infeksi Ganda Begomovirus Dan Crinivirus Pada Tanaman Tomat Di Kabupaten Magelang , Jawa Tengah (Double Infections of Begomovirus and Crinivirus on Tomato Plants at Magelang Regency, Central Java)

Cited by 9 publications

References 5 publications

Effect of Screen House on Disease Severity and Coat Protein Diversity of Begomovirus-infected Capsicum frutescens L. ‘Cempluk’ from Indonesia

Effect of Screen House on Disease Severity and Coat Protein Diversity of Begomovirus-infected Capsicum frutescens L. ‘Cempluk’ from Indonesia

A recombinant DNA‐satellite associated with Pepper yellow leaf curl Indonesia virus in highland area

Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai

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