Inferring the Geometry of Fourth-Period Metallic Elements in Arabidopsis thaliana Seeds using Synchrotron-Based Multi-Angle X-ray Fluorescence Mapping

Young, Lester; Westcott, Neil D.; Christensen, Colleen; Terry, Jeff; Lydiate, Derek J.; Reaney, Martin J. T.

doi:10.1093/aob/mcm205

Cited by 12 publications

(24 citation statements)

References 25 publications

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“…Even though, this paper provided a clear example of the usefulness of 3D elemental imaging through fluorescence SR-µCT, it is also true that this technique is time-consuming and not suitable for screening of a large number of mutants. To overcome this difficulty, Young et al (2007) proposed a method to infer the geometry of metals in Arabidopsis seeds by collecting 2D maps at few orientations. Laterally resolved XRF and XANES have also been used to assess the distribution and speciation of As in white and brown rice grains (Meharg et al 2008).…”

Section: Sr-techniques For the Investigation Of Metals In Seedsmentioning

confidence: 99%

Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives

2009

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Spectroscopic approaches to plant and soil sciences have provided important information for several decades. However, many of these approaches suffered from a number of limitations and drawbacks especially in terms of spatial resolution and requirements for sample preparation. The advent of dedicated synchrotron facilities, that allow the exploitation of the particular qualities of synchrotron radiation as a research tool, has revolutionised the way we approach

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Section: Sr-techniques For the Investigation Of Metals In Seedsmentioning

confidence: 99%

Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives

2009

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“…In recent decades, imaging of elements in plant seeds or grains has been carried out using X-ray fluorescence (XRF), [7][8][9][10][11][12] particle induced X-ray emission (PIXE), [12][13][14][15][16][17][18] and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). 19,20 The samples in these research studies covered rice, 7,12 wheat, 19,20 soybean 17 and other plant seeds.…”

Section: Introductionmentioning

confidence: 99%

Distribution of the Elements in Cotyledon, Embryonic Axis, and Testa of Peanut Seeds Obtained by ICP-MS with Microwave Acid Digestion

2013

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Eighteen elements in the cotyledon, the embryonic axis, and the testa of peanut seeds were determined by inductively coupled plasma mass spectrometry (ICP-MS) after microwave acid digestion, while the gravimetric standard addition with internal standard was applied for the calibration of the elemental concentrations. The detection limit and the procedure blank value for each element were low enough to ensure the precise analysis of the elements, with a relative expanded uncertainty of less than 5%. The concentrations of the elements in peanut seed samples covered 6 orders of magnitude from approximately 0.01 mg kg -1 of Co to approximately 7000 mg kg -1 of K. The correlation coefficient factor was around 0.98 for the elemental concentrations in peanut seeds grown in Japan and those grown in China, indicating a good correlation. Most of the elements distributed in the cotyledon in large amounts because of the cotyledon's relatively high mass fraction. By contrast, Na, Ca, Fe, Co, Ni, Cu, Ga, Sr, Cd, and Ba were apparently enriched in the testa and the relative enrichment factor (REF) values of the elements were over 4. The relative enrichment of Mo, Fe, Zn and other elements was observed in the embryonic axis samples with REF values over 2. The relative enrichment of Cd in the testa of peanut seed indicates that about 15 to 25% of the Cd intake through peanut seeds could be effectively lowered by removal of the testa (roughly 2.5 to 3.5% of the peanut seed).

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“…, 2003; Vogel‐Mikuš et al. , 2007) and synchrotron X‐ray fluorescence (S‐XRF) mapping (Young et al. , 2007; Meharg et al.…”

Section: Introductionmentioning

confidence: 99%

“…However, these techniques can only provide a rough and semiquantitative estimate of metal distribution and concentration. Much more powerful combinations of X-ray analysis and electron microscopy techniques, such as energy-dispersive X-ray (EDX) analysis (Otegui et al, 2002), microproton-induced X-ray emission (micro-PIXE, Bhatia et al, 2003;Vogel-Mikuš et al, 2007) and synchrotron X-ray fluorescence (S-XRF) mapping (Young et al, 2007;Meharg et al, 2008;Lombi et al, 2009), as well as high-resolution secondary ion mass spectrometry (SIMS) (Heard et al, 2001;Feeney et al, 2003;Moore et al, 2010) are currently available methods for better localization of individual components. In particular, Kim et al (2006) convincingly demonstrated the potential of combining S-XRF microtomography in relating molecular studies on micronutrient transport to mapping element distribution in Arabidopsis seeds.…”

Section: Introductionmentioning

confidence: 99%

Stable isotope labelling and zinc distribution in grains studied by laser ablation ICP‐MS in an ear culture system reveals zinc transport barriers during grain filling in wheat

2010

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Summary Zinc (Zn) deficiency has been recognized as a potential risk for human health in many developing regions where staple food with low micronutrient density represents a major proportion of the diet. The success of strategies to increase Zn content in the edible part of crops requires better understanding of Zn transport to, and distribution within, the grains. The transfer of Zn from the growth medium to wheat (Triticum aestivum) grains in an ear culture system was investigated by using the stable Zn isotope 70Zn, and the spatial distribution of Zn within the grains was studied by laser ablation‐inductively coupled plasma‐mass spectrometry (LA‐ICP‐MS). Zinc was readily transported in the stem up to the rachis. More Zn accumulated in the stem when higher amounts of Zn were supplied to the medium. Once Zn was transported into the grain, Zn accumulated particularly in the crease vascular tissue. The gradient of 70Zn concentration between crease vascular tissue, aleurone layer and endosperm demonstrates that Zn is distributed within grain through the crease phloem. These results suggest that two barriers of Zn transport into wheat grains may exist: between the stem tissue rachis and the grain, and the maternal and filial tissues in the grain.

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Inferring the Geometry of Fourth-Period Metallic Elements in Arabidopsis thaliana Seeds using Synchrotron-Based Multi-Angle X-ray Fluorescence Mapping

Cited by 12 publications

References 25 publications

Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives

Synchrotron-based techniques for plant and soil science: opportunities, challenges and future perspectives

Distribution of the Elements in Cotyledon, Embryonic Axis, and Testa of Peanut Seeds Obtained by ICP-MS with Microwave Acid Digestion

Stable isotope labelling and zinc distribution in grains studied by laser ablation ICP‐MS in an ear culture system reveals zinc transport barriers during grain filling in wheat

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