Infestation of Stored Saw Palmetto Berries by Cadra Cautella (Lepidoptera: Pyralidae) and the Host Paradox in Stored-Product Insects

Arbogast, Richard T.; Chini, Shahpar R.; Kendra, Paul E.

doi:10.1653/0015-4040(2005)088[0314:iosspb]2.0.co;2

Cited by 16 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although several lepidopteran pests attack date fruits, the almond moth, (Cadra cautella) (Walker) (Lepidoptera: Pyralidae) is a major date fruit pest [6][7][8]. The C. cautella damage date fruits when the fruit is still on the tree, infest date fruits in storehouses, and can produce several generations per year under suitable environmental conditions [9].…”

Section: Introductionmentioning

confidence: 99%

RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella

et al. 2021

View full text Add to dashboard Cite

Vitellogenins, major yolk protein precursors, play an essential role in the reproduction and spread of all oviparous species, including insects. To investigate reproductive strategies of the warehouse moth Cadra cautella at the molecular level, a partial transcript of the C. cautella vitellogenin (CcVg) gene was extended through the rapid amplification of cDNA ends PCR and sequenced. The complete CcVg mRNA transcript was 5,334 bp long, which encoded a protein of 1,778 amino acids, including the first 14 amino acids of the signal peptide. The deduced CcVg protein contained a putative cleavage site (RTRR) at the amino-terminal side, similar to several other insect species. DGQR and GI/LCG motifs were present at the CcVg gene C-terminus, followed by nine cysteine residues. CcVg harbored 131 putative phosphorylation sites, numbering 84, 19, and 28 sites for serine, threonine, and tyrosine, respectively. The transcript showed a great resemblance with other lepidopteran Vgs. CcVg protein analysis revealed three conserved regions: 1) vitellogenin-N domain, 2) DUF 1943 (domain of unknown function), and 3) a von Willebrand factor type D domain. Additionally, sex, stage-specific, and developmental expression profiles of the CcVg gene were determined through RT-PCR. The Vg was first expressed in 22-day-old female larvae, and its expression increased with growth. The phylogenetic analysis based on different insect Vgs revealed that the CcVg exhibited close ancestry with lepidopterans. The CcVg-based RNAi experiments were performed, and the effects were critically evaluated. The qRT-PCR results showed that CcVg-based dsRNA suppressed the Vg gene expression up to 90% at 48 h post-injection. Moreover, CcVg-based RNAi effects resulted in low fecundity and egg hatchability in the CcVg-based dsRNA-treated females. The females laid eggs, but because of insufficient yolk protein availability the eggs could not succeed to hatch. The significant difference in the fecundity and hatchability unveils the importance of CcVg gene silencing and confirmed that the Vg gene plays a key role in C. cautella reproduction and it has the potential to be used as a target for RNAi-mediated control of this warehouse pest.

show abstract

Section: Introductionmentioning

confidence: 99%

RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Approximately 70% of moths are linked to stored product infestations. The almond moth, C. cautella (Walker), is an economically important pest of dates [6, 12, 45]. Recent studies have focused on its biology and ecology, and have proposed several management strategies to control these pests, including use of botanical extracts [46], heat treatments [47], freezing effects [48], essential oil extract [49, 50], and modified atmosphere [12, 51].…”

Section: Discussionmentioning

confidence: 99%

“…These pests include the almond moth, Cadra cautella (Walker) (Lepidoptera: Pyralidae) and the sawtoothed grain beetle, Oryzaephilus surinamensis [1]. In the Middle East as well as in many other regions of the world, C. cautella is a destructive polyphagous storage pest of date fruits, cereals, dried fruits, ground nuts, and maize [6–8]. The life cycle of C. cautella is short with many generations per year and a single female can produce 213 and 422 eggs/female, when reared on artificial diet and “khodari” date fruits, respectively [7, 9–12].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the almond moth, Cadra cautella, female abdominal tissues and identification of reproduction control genes

et al. 2019

View full text Add to dashboard Cite

BackgroundThe almond moth, Cadra cautella is a destructive pest of stored food commodities including dates that causes severe economic losses for the farming community worldwide. To date, no genetic information related to the molecular mechanism/strategies of its reproduction is available. Thus, transcriptome analysis of C. cautella female abdominal tissues was performed via next-generation sequencing (NGS) to recognize the genes responsible for reproduction.ResultsThe NGS was performed with an Illumina Hiseq 2000 sequencer (Beijing Genomics Institute: BGI). From the transcriptome data, 9,804,804,120 nucleotides were generated and their assemblage resulted in 62,687 unigenes. The functional annotation analyses done by different databases, annotated, 27,836 unigenes in total. The transcriptome data of C. cautella female abdominal tissue was submitted to the National Center for Biotechnology Information (accession no: PRJNA484692). The transcriptome analysis yielded several genes responsible for C. cautella reproduction including six Vg gene transcripts. Among the six Vg gene transcripts, only one was highly expressed with 3234.95 FPKM value (fragments per kilobase per million mapped reads) that was much higher than that of the other five transcripts. Higher differences in the expression level of the six Vg transcripts were confirmed by running the RT-PCR using gene specific primers, where the expression was observed only in one transcript it was named as the CcVg.ConclusionsThis is the first study to explore C. cautella reproduction control genes and it might be supportive to explore the reproduction mechanism in this pest at the molecular level. The NGS based transcriptome pool is valuable to study the functional genomics and will support to design biotech-based management strategies for C. cautella.

show abstract

“…This should favor a higher investment in survival and flight capability for future reproduction at the cost of lower egg production, in comparison to asexuals. The latter live in grain stores and mills, where host distribution is aggregated ( Bowditch & Madden, 1996 ) and the amplitude of host density variation is very large ( Campbell & Arbogast, 2004 ; Arbogast & Chini, 2005 ; Arbogast, Chini & Kendra, 2005 ; Belda & Riudavets, 2013 ). These environmental conditions should favor higher investment in the production of eggs available for immediate reproduction rather than survival and flight capability.…”

Section: Introductionmentioning

confidence: 99%

Adaptations to different habitats in sexual and asexual populations of parasitoid wasps: a meta-analysis

Amat

Alphen

Kacelnik

et al. 2017

PeerJ

View full text Add to dashboard Cite

BackgroundCoexistence of sexual and asexual populations remains a key question in evolutionary ecology. We address the question how an asexual and a sexual form of the parasitoid Venturia canescens can coexist in southern Europe. We test the hypothesis that both forms are adapted to different habitats within their area of distribution. Sexuals inhabit natural environments that are highly unpredictable, and where density of wasps and their hosts is low and patchily distributed. Asexuals instead are common in anthropic environments (e.g., grain stores) where host outbreaks offer periods when egg-load is the main constraint on reproductive output.MethodsWe present a meta-analysis of known adaptations to these habitats. Differences in behavior, physiology and life-history traits between sexual and asexual wasps were standardized in term of effect size (Cohen’s d value; Cohen, 1988).ResultsSeeking consilience from the differences between multiple traits, we found that sexuals invest more in longevity at the expense of egg-load, are more mobile, and display higher plasticity in response to thermal variability than asexual counterparts.DiscussionThus, each form has consistent multiple adaptations to the ecological circumstances in the contrasting environments.

show abstract

Infestation of Stored Saw Palmetto Berries by Cadra Cautella (Lepidoptera: Pyralidae) and the Host Paradox in Stored-Product Insects

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 16 publications

References 15 publications

RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella

RNAi-mediated silencing of vitellogenin gene curtails oogenesis in the almond moth Cadra cautella

Transcriptome analysis of the almond moth, Cadra cautella, female abdominal tissues and identification of reproduction control genes

Adaptations to different habitats in sexual and asexual populations of parasitoid wasps: a meta-analysis

Contact Info

Product

Resources

About