Inflammation in Hypervolemic Hemodialysis Patients: The Roles of RelB and Caspase-4

Ulrich, Christof; Canim, Zeynep; Herberger, Eva; Girndt, Matthias; Fiedler, Roman

doi:10.3390/ijms242417550

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…The patient characteristics of this cohort were reported recently [21]. In short, the hydration status of the patients was analyzed by bioelectric impedance vector analysis (BIVA).…”

Section: Resultsmentioning

confidence: 99%

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Hypervolemia in Dialysis Patients Impairs STAT3 Signaling and Upregulates miR-142-3p: Effects on IL-10 and IL-6

Ulrich,

Fiedler,

Herberger

et al. 2024

IJMS

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Fluid overload in hemodialysis patients (HD) has been proven to be associated with inflammation. Elevated levels of the pro-inflammatory cytokine interleukin-6 (IL-6) appear to be inadequately counterbalanced by the anti-inflammatory cytokine interleukin-10 (IL-10). We initiated a cross-sectional study enrolling 40 HD patients who were categorized by a bioimpedance measurement in normovolemic (N; 23) and hypervolemic (H; 17) groups to test whether IL-10- and IL-6-related signal transduction pathways (signal transducer of transcript 3: STAT3) and/or a post-transcriptional regulating mechanism (miR-142) are impaired by hypervolemia. IL-10/IL-6 transcript and protein production by PBMCs (peripheral blood mononuclear cells) were determined. Phospho-flow cytometry was used to detect the phosphorylated forms of STAT3 (pY705 and pS727). miR-142-3p/5p levels were detected by qPCR. Hypervolemic patients were older, more frequently had diabetes, and showed higher CRP levels. IL-10 transcripts were elevated in H patients but not IL-10 protein levels. In spite of the elevated mRNA expression of the suppressor of cytokine expression 3 (SOCS3), IL-6 mRNA and protein expression were increased in immune cells of H patients. The percentage of cells staining positive for STAT3 (pY705) were comparable in both groups; in STAT3 (pS727), however, the signal needed for full transactivation was decreased in H patients. miR-142-3p, a proven target of IL-10 and IL-6, was significantly elevated in H patients. Insufficient phosphorylation of STAT3 may impair inflammatory and anti-inflammatory cytokine signaling. How far degradative mechanisms induced by elevated miR-142-3p levels contribute to an inefficient anti-inflammatory IL-10 signaling remains elusive.

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“…The patient characteristics of this cohort were reported recently [21]. In short, the hydration status of the patients was analyzed by bioelectric impedance vector analysis (BIVA).…”

Section: Resultsmentioning

confidence: 99%

“…The study population was described recently [21]. In short, there were 40 patients on maintenance hemodialysis at the Nephrology Outpatient Dialysis Center of the Department of Internal Medicine II of the University Halle-Wittenberg were enrolled in the cross-sectional study.…”

Section: Study Populationmentioning

confidence: 99%

Hypervolemia in Dialysis Patients Impairs STAT3 Signaling and Upregulates miR-142-3p: Effects on IL-10 and IL-6

Ulrich,

Fiedler,

Herberger

et al. 2024

IJMS

Self Cite

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Blood pressure control with active ultrafiltration measures and without the use of antihypertensives is essential for survival in patients with CKD in hemodiafiltration and hemodialysis programs. A prospective multicenter observational study.

Mora-Bravo,

Morales,

Rojas

et al. 2024

Preprint

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Background High blood pressure is a prevalent condition in patients with chronic kidney disease on hemodialysis. Adequate control of high blood pressure is essential to reduce deaths in this group of patients. The present study aimed to observe mortality prospectively in a group of patients in hemodialysis and hemodiafiltration programs in whom the use of antihypertensives was optimized with the point of care dry weight (POCW) technique. Methods The present observational, prospective study was carried out at the Pafram Hemodiafiltration Unit in Morona Santiago, Ecuador, and the Hemodialysis Unit of the Fundación Renal del Ecuador in Guayaquil, Ecuador, from August 2019 to December 2023. Patients who were receiving hemodiafiltration were included. Weight was optimized with POCW. Group 1 included patients whose antihypertensive agents were not required to control blood pressure with a value less than 150/85 mmHg predialysis at eight weeks of POCW. In group 2, the patients required antihypertensive therapy. Clinical data, demographic data, mortality data, treatment descriptions, and routine laboratory test results during dialysis were included. The sample was nonprobabilistic. Survival analysis was carried out for the study groups. The log-rank test (Mantel-Cox) was used for survival comparisons. Results The study included 106 patients. Optimal blood pressure control without antihypertensive treatment was achieved in 52 patients (49.1%) (Group 1). In 54 patients (50.9%), antihypertensive agents were required (Group 2). There was more significant mortality in the group that received antihypertensives: 11 patients in group 1 (21.2%) versus 25 patients in group 2 (46.3%) (P = 0.005). Survival was more significant in group 1, with an HR of 2.2163 (1.125–4.158) (P = 0.0243). Conclusion Blood pressure control with active ultrafiltration measures and without the use of antihypertensives is essential for survival in patients with CKD in hemodiafiltration and hemodialysis programs.

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Inflammation in Hypervolemic Hemodialysis Patients: The Roles of RelB and Caspase-4

Cited by 2 publications

References 33 publications

Hypervolemia in Dialysis Patients Impairs STAT3 Signaling and Upregulates miR-142-3p: Effects on IL-10 and IL-6

Hypervolemia in Dialysis Patients Impairs STAT3 Signaling and Upregulates miR-142-3p: Effects on IL-10 and IL-6

Blood pressure control with active ultrafiltration measures and without the use of antihypertensives is essential for survival in patients with CKD in hemodiafiltration and hemodialysis programs. A prospective multicenter observational study.

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