Inflammation induced by inactivated Aeromonas hydrophila in Nile tilapia fed diets supplemented with Saccharomyces cerevisiae

Reque, Valeska Regina; Moraes, Julieta Rodini Engrácia de; Belo, Marco Antônio de Andrade; Moraes, Flávio Ruas de

doi:10.1016/j.aquaculture.2009.12.014

Cited by 48 publications

(55 citation statements)

References 39 publications

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“…In agreement with the findings of Reque et al (2010) in tilapia fed supplemented diet with Saccaromyces cerevisiae, the acute inflammation induced by A. hydrophila did not show alterations in hematocrit.…”

Section: Discussionsupporting

confidence: 79%

“…The importance of thrombocytes as a phogocytic-cell has been reported in birds, Gallus gallus domesticus (Grecchi et al, 1980;Kajigaya et al, 1985), bullfrog, Lithobathes catesbeianus (Ishida et al, 1985;Dias and Sinhorini, 1991;Penha et al, 1996) and tilapia (Suzuki, 1986;Matushima and Mariano, 1996). Similarly to the present results, Reque et al (2010) observed increased number of thrombocytes accompanied by lowered number of macrophages, neutrophils and lymphocytes in the inflammatory exudates of tilapia fed Saccharomyces cerevisae. Zanuzzo (2010) evaluated the respiratory burst of macrophages in Brycon amazonicus, kept in water containing A. vera, and observed an increase in the respiratory activity of leucocytes .…”

Section: Discussionsupporting

confidence: 73%

“…The circulating blood cells that migrate into tissues to participate in inflammatory response include thrombocytes, lymphocytes, macrophages and granulocytes, corroborating previous studies. (Bozzo et al, 2007;Reque et al, 2010;Salvador et al, 2012;Claudiano, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

et al. 2015

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This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.Keywords: inflammation, carrageenin, swim bladder, propolis, Aloe.Inflamação aguda e resposta hematológica em tilápias do Nilo alimentadas com extratos naturais de própolis e Aloe barbadensis suplementados na dieta ResumoEste estudo avaliou a resposta inflamatória aguda induzida por carragenina na bexiga natatóriade tilápia do Nilo suplementada com a mistura dos extratos naturais de própolis e Aloe barbadensis (1:1), nas concentrações de 0,5%, 1% e 2% na dieta durante o período de 15 dias. Trinta e seis peixes foram distribuídos em quatro tratamentos com três repetições: peixes suplementados com 0,5% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 1% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina; peixes suplementados com 2% da mistura dos extratos de própolis e Aloe (1:1) injetados na bexiga natatória com 500 µg de carragenina e peixes não suplementados injetados na bexiga natatória com 500 µg de carragenina. Seis horas após as injeções foram coletadas amostras de sangue e exsudato da bexiga natatória dos peixes. Foi observado aumento na contagem de leucócitos no exsudato da bexiga natatória de peixes suplementados com os extratos de própolis e Aloe injetados com carragenina. As células mais frequentes foram os macrófagos seguidos pelos leucócitos granulares, trombócitos e linfócitos. A suplementação com própolis e Aloe a 0,5% provocou aumento significativo no número de células no foco inflamatório, principalmente dos macrófagos, células responsáveis pela atividade fagocitária nos tecidos, agente da resposta imune inata nos peixes.Palavras-chave: inflamação,...

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 73%

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Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

et al. 2015

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“…In fish supplemented with the ascorbyl polyphosphate and at 20 kg m -3 stocking density, the plasma cortisol concentration was lower, which, at least partly, favored cell accumulation on the coverslip. In teleost fish, the glucocorticoid activity of cortisol has proved to suppress immune defense responses, acting mainly on the lymphocyte populations which coordinate the release of inflammatory mediators such as cytokines (Reque et al, 2010). However, pacus supplemented with ascorbyl polyphosphate at 5 kg m -3 stocking density showed a greater formation of Langhans cells, which have greater activity of acid phosphatase and succinic hydrogenase, a better developed Golgi system, and more orderly distribution of cell organelles (Anderson et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo

Moraes

Soares

et al. 2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effect of food supplementation with vitamin C on macrophage and multinucleated giant cell (MGC) activities of pacus at two stocking densities. The experiment was carried out in a 2x2x3 split-plot factorial arrangement with: 0 and 500 mg kg -1 vitamin C; 5 and 20 kg m -3 stocking densities; and evaluation times at 3, 6, and 12 days after the subcutaneous implantation of glass coverslips (DPI). The number of macrophages and MGC, as well as cortisol and glucose plasma levels were determined. The number of macrophages and MGC with two to five nuclei was significantly greater in fish supplemented with vitamin C at 5 kg m -3 stocking density at 3 DPI in comparison to nonsupplemented ones. The macrophage and MGC counts were lower in fish with high-plasma cortisol concentration. Supplementation with 500 mg vitamin C benefits macrophage activity on foreign-body inflammation, and high-cortisol concentration has suppressive effects on this response.Index terms: Piaractus mesopotamicus, ascorbic acid, glucocorticoid, inflammation, Langhans cells, multinucleated giant cells. Vitamina C e cortisol endógeno em resposta inflamatória tipo corpo estranho em pacusResumo -O objetivo deste trabalho foi avaliar o efeito da suplementação com vitamina C no aumento da atividade de macrófagos e células gigantes multinucleadas (CGM), em pacus mantidos em duas densidades de estocagem. O experimento foi realizado em parcelas subdivididas, em arranjo fatorial 2x2x3 com: 0 e 500 mg kg -1 de vitamina C; densidade de estocagem de 5 e 20 kg m -3 ; e tempos de avaliação de 3, 6 e 12 dias após o implante subcutâneo (DPI) de lamínulas de vidro. Foram determinados o número de macrófagos e de CGM, bem como os níveis de cortisol e glicose no plasma. O número de macrófagos e de CGM com 2 a 5 núcleos foi significativamente maior nos peixes suplementados com vitamina C, à densidade 5 kg m -3 , aos 3 DPI, em relação aos não suplementados. Constatou-se menor quantidade de macrófagos e CGM em peixes com baixa concentração plasmática de cortisol. A suplementação com 500 mg de vitamina C por quilograma de ração beneficia a atividade de macrófagos em inflamações do tipo corpo estranho, e a elevada concentração de cortisol circulante tem efeito supressor nesta resposta.Termos para indexação: Piaractus mesopotamicus, ácido ascórbico, glicocorticoide, inflamação, células de Langhans, células gigantes multinucleadas.

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“…In acute infl ammatory reactions occurs signifi cant increase in the number of neutrophils that are recruited by chemotaxis to act in the infl amed site [29,30].…”

Section: Discussionmentioning

confidence: 99%

Clinical safety of amino acids and vitamins administered through parenteral route

Moraes¹,

Scherma²,

Prado³

et al. 2017

Int J Vet Sci Res

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The parenteral use of amino acids and vitamins has been used to improve performance in animal production. However, few studies demonstrating the toxicity of these compounds. The present investigation evaluated the toxicological and clinical safety of the injectable supplement compound by amino acids and vitamins, administered by intramuscular route in Wistar rats. 56 females (± 220g), pubescent virgins, were randomly divided into seven groups (n=8): Treated with dose of 0.2, 2 and 4 mL, sampled 24 and 96 hours post-treatment (HPT), totaling six groups and one control group (Physiology Standard). Signifi cant increase in the enzymatic activity of serum ALT, AST, LDH and ALP was observed in animals treated with 2 and 4 mL (24 HPT). However, these changes returned to baseline levels 96 HPT, except in serum AST and ALP in animals treated with 4 mL. CK-NAC showed dose-response effect, since the higher compound dose was followed by proportional increase in the circulating levels of this enzyme, associated to muscle degeneration and necrosis area in rats treat with 4mL at 96 HPT. The evaluation of hepatic function did not result in signifi cant changes in the serum levels of triglycerides, cholesterol, albumin, urea and creatinine in the comparison between rats treated with 0.2 mL and control rats, demonstrating the innocuousness of this treatment on the activity of liver and kidney. However, deleterious effects were observed in animals subjected to doses of 2 and 4 mL, including observed increased splenic and hepatosomatic response, signifi cant decrease in circulating albumin levels and signifi cant leukocytosis, which is characterized by neutrophilia, followed by lymphopenia demonstrating the compound irritating effect on muscle tissue.

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Inflammation induced by inactivated Aeromonas hydrophila in Nile tilapia fed diets supplemented with Saccharomyces cerevisiae

Cited by 48 publications

References 39 publications

Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Clinical safety of amino acids and vitamins administered through parenteral route

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