Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes

Yamamoto, Seiji; Niida, Shumpei; Azuma, Erika; Yanagibashi, Tsutomu; Matsushima, Masashi; Huang, Ting; Sagara, Hiroshi; Higaki, Sayuri; Ikutani, Masashi; Nagai, Yoshinori; Takatsu, Kiyoshi; Miyazaki, Kenji; Hamashima, Takeru; Mori, Hisashi; Matsuda, Naoyuki; Ikoma, Yoko; Sasahara, Masakiyo

doi:10.1038/srep08505

Cited by 139 publications

(137 citation statements)

References 65 publications

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“…A number of studies have demonstrated that RNA, DNA, and proteins are encapsulated in EVs as bioactive molecules (Table I) (Al-Nedawi et al, 2009;Cai et al, 2013;Waldenstrom et al, 2012;Yamamoto et al, 2015). Some reports have suggested that the bioactive molecules encapsulated in EVs play important roles in intercellular communication, and in specific signal transductions in the pathophysiological states of cells both in vitro and in vivo (Kawamoto et al, 2012;Tominaga et al, 2015;Yamamoto et al, 2015).…”

Section: Bioactive Molecules Encapsulated In Evsmentioning

confidence: 99%

“…Some reports have suggested that the bioactive molecules encapsulated in EVs play important roles in intercellular communication, and in specific signal transductions in the pathophysiological states of cells both in vitro and in vivo (Kawamoto et al, 2012;Tominaga et al, 2015;Yamamoto et al, 2015).…”

Section: Bioactive Molecules Encapsulated In Evsmentioning

confidence: 99%

“…Extracellular vesicles (EVs) are small membranous globules that are released from many cells including endothelial cells, leukocytes, and cancer cells (Kawamoto et al, 2012;Leroyer et al, 2007;Yamamoto et al, 2015). Clinically, the number of EVs has been observed to increase in the bloodstream of patients suffering from acute and chronic inflammation evoked by diseases such as sepsis, stroke, preeclampsia, atherosclerosis, diabetes mellitus, metabolic syndrome, and cancer Boulanger et al, 2006;Koga et al, 2005;Mostefai et al, 2008;Petrozella et al, 2012;Simak et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Clinically, the number of EVs has been observed to increase in the bloodstream of patients suffering from acute and chronic inflammation evoked by diseases such as sepsis, stroke, preeclampsia, atherosclerosis, diabetes mellitus, metabolic syndrome, and cancer Boulanger et al, 2006;Koga et al, 2005;Mostefai et al, 2008;Petrozella et al, 2012;Simak et al, 2006). Several studies have reported that EVs mediate intercellular communica-tions by releasing encapsulated materials such as messenger RNAs (mRNAs), microRNAs (miRNAs), and proteins, to target cells (Al-Nedawi et al, 2009Fujita et al, 2014;Kawamoto et al, 2012;Tominaga et al, 2015;Waldenstrom et al, 2012;Yamamoto et al, 2015). This review summarizes and discusses the current understanding of the pathophysiological role of EVs in various diseases.…”

Section: Introductionmentioning

confidence: 99%

“…When the plasma membrane is activated by extrinsic stimuli such as inflammatory cytokines and lipopolysaccharide (LPS), membranous globular objects of submicron to micron sizes (0.05-3 μm) are shed from the plasma membrane surface, and subsequently released (Morhayim et al, 2014;Raposo and Stoorvogel, 2013;Yamamoto et al, 2015). These globular objects comprised of the plasma membrane are categorized as microparticles/ microvesicles among EVs (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Significance of Extracellular Vesicles: Pathobiological Roles in Disease

Yamamoto

Azuma

Matsushima

et al. 2016

Cell Struct. Funct.

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ABSTRACT. Over the past decade, many studies have been conducted on extracellular vesicles (EVs) in the fields of basic and clinical research. EVs are small sized membranous vesicles generated from many type of cells upon activation by environmental stresses such as heat, hypoxia, and irradiation. EVs theoretically consist of microparticles/microvesicles, exosomes, and apoptotic bodies by different productive mechanisms. Clinically, EVs are observed in the blood stream of patients suffering from acute and chronic inflammation evoked by various diseases, and number of EVs in blood flow is often dependent on the inflammatory status and severity of the diseases. To date, it has been reported that small molecules such as RNAs and proteins are encapsulated in EVs; however, the functions of EVs are still unclear in the biological, pathological, and clinical aspects. In this review, we summarize and discuss the biogenesis-based classification, expected function, and pathobiological activities of EVs.

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Section: Bioactive Molecules Encapsulated In Evsmentioning

confidence: 99%

Section: Bioactive Molecules Encapsulated In Evsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Significance of Extracellular Vesicles: Pathobiological Roles in Disease

Yamamoto

Azuma

Matsushima

et al. 2016

Cell Struct. Funct.

Self Cite

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Heterogeneity of microvesicles from cancer cell lines under inflammatory stimulation with TNF‐α

Gieseler

Plattfaut

Quecke

et al. 2018

Cell Biology International

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Microvesicles (MVs) represent a subgroup of extracellular vesicles (EVs) emerging from various cells by blebbing of their outer membrane. Therefore, they share features such as membrane composition and antigenicity with their parental cells. Released by many immune and tumor cells, MVs act as intercellular messengers, account for horizontal gene transfer and can activate the coagulation system. With the aim to investigate their relevance for tumor cell biology, we characterized MVs released by human tumor cell lines of various origins in the absence or presence of TNF-α. After stimulation, we used the combination of low and high-speed centrifugation to enrich MVs from cell culture supernatants. We analyzed the presentation of phosphatidylserine (PS) and tissue factor (TF) activity on the cell surface and investigated their potency to induce tumor cell migration. In all tumor cell lines, TNF-α stimulation enhanced the release of MVs. While the expression of PS was universally increased, an elevated activity of procoagulant TF could be detected on MVs from lung, pancreatic, and colon carcinoma, but not from breast and ovarian cancer cell lines. Functionally, TNF-α stimulation significantly increased the potency of MVs to induce tumor cell migration. In conclusion, inflammatory conditions promote the release of MVs with increased procoagulant activity from tumor cell lines in vitro. PS-containing and TF-expressing MVs may account for systemic activation of the coagulation system as seen in cancer patients and, since they induce tumor cell migration, they may serve as biomarkers for tumor progression.

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Proteomics of cell–cell interactions in health and disease

et al. 2015

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The mechanisms of cell-cell communications are now under intense study by proteomic approaches. Proteomics has unraveled changes in protein profiling as the result of cell interactions mediated by ligand/receptor, hormones, soluble factors, and the content of extracellular vesicles. Besides being a brief overview of the main and profitable methodologies now available (evaluating theory behind the methods, their usefulness, and pitfalls), this review focuses on-from a proteome perspective-some signaling pathways and post-translational modifications (PTMs), which are essential for understanding ischemic lesions and their recovery in two vital organs in mammals, the heart, and the kidney. Knowledge of misdirection of the proteome during tissue recovery, such as represented by the convergence between fibrosis and cancer, emerges as an important tool in prognosis. Proteomics of cell-cell interaction is also especially useful for understanding how stem cells interact in injured tissues, anticipating clues for rational therapeutic interventions. In the effervescent field of induced pluripotency and cell reprogramming, proteomic studies have shown what proteins from specialized cells contribute to the recovery of infarcted tissues. Overall, we conclude that proteomics is at the forefront in helping us to understand the mechanisms that underpin prevalent pathological processes.

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Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes

Cited by 139 publications

References 65 publications

Significance of Extracellular Vesicles: Pathobiological Roles in Disease

Significance of Extracellular Vesicles: Pathobiological Roles in Disease

Heterogeneity of microvesicles from cancer cell lines under inflammatory stimulation with TNF‐α

Proteomics of cell–cell interactions in health and disease

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