Inflammation, oxidative stress and genotoxicity responses to biodiesel emissions in cultured mammalian cells and animals

Scholten, Rebecca Harnung; Roursgaard, Martin; Krais, Annette M.

doi:10.1080/10408444.2020.1762541

Cited by 31 publications

(19 citation statements)

References 130 publications

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“…Hence, the physicochemical characteristics of the emitted particles, such as content of PAH, metals and ratio of elemental and organic carbon [14,17,18], depend on engine combustion conditions. Particle size and thereby specific surface area (SSA) is a driver of pulmonary inflammation [19] and acute phase response [20,21] whereas certain metals and PAH, as well as ROS formation are linked to genotoxicity [16,22,23]. Several PAH compounds including benzo[a]pyrene are classified as carcinogenic or possibly carcinogenic by IARC [24].…”

Section: Introductionmentioning

confidence: 99%

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

et al. 2020

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Background: Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from three fuel types were compared at 13% engine intake O 2 concentration: MK1 ultra low sulfur diesel (DEP13) and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O 2 concentration. We evaluated physicochemical properties and histopathological, inflammatory and genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in mice compared to reference diesel particles and carbon black.

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Section: Introductionmentioning

confidence: 99%

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

et al. 2020

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“…The EC fraction was higher for DEP13 compared to HVO13 and RME13. [21,23] 17 [16,19] 16 [15,17] 21 [19,23] 15 [14,16] 15 [14,15] Estimated specific surface area (SSA) a The average exhaust PM mass concentration (mg/m 3 ), before dilution and particle mobility diameter (nm) with ± 1 std. dev.…”

Section: Electron Microscopymentioning

confidence: 99%

“…Hence, the physicochemical characteristics of the emitted particles, such as content of PAH, metals and ratio of elemental and organic carbon [14,16,17], depend on engine combustion conditions. Particle size and thereby speci c surface area (SSA) is a driver of pulmonary in ammation [18] and acute phase response [19,20] whereas certain metals and PAH, as well as ROS formation are linked to genotoxicity [21,22,23]. Several PAH compounds including benzo[a]pyrene are classi ed as carcinogenic or possibly carcinogenic by IARC [24].…”

Section: Introductionmentioning

confidence: 99%

“…Emissions also depend on fuel type [17,25,26,27], and recently, renewable diesel fuels have been introduced on large scales to replace fossil diesel [28,29,30]. There is incomplete knowledge on the potential adverse health effects of emissions from these new types of renewable diesel fuels, especially regarding gentoxicity and carcinogenicity [23]. Renewable diesel fuels are based on vegetable oils, animal fats or waste products and are used in conventional engines as full substitution or in blends.…”

Section: Introductionmentioning

confidence: 99%

“…cardiovascular and systemic response between biodiesel and petroleum diesel. Recently, Møller et al[23] reviewed the available literature on cell and animal studies, and concluded that the evidence of biodiesel-induced inflammatory, oxidative stress and genotoxicity response is weak and inconsistent between studies. Furthermore, engine type, combustion conditions and after-treatment have been identified as major determinants of the chemical composition of the diesel exhaust particles[47,81].…”

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Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

Bendtsen

Gren²,

Malmborg³

et al. 2020

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Background Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from three fuel types were compared at 13% engine intake O 2 concentration: MK1 ultra low sulfur diesel (DEP13) and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O 2 concentration. We evaluated physicochemical properties and histopathological, inflammatory and genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in mice compared to reference diesel particles and carbon black. Results Moderate variations were seen in physical properties for the five particles: primary particle diameter: 15-22 nm, specific surface area: 152-222 m 2 /g, and count median mobility diameter: 55-103 nm. Larger differences were found in chemical composition: organic carbon/total carbon ratio (0.12-0.60), polycyclic aromatic hydrocarbon content (1-27 mg/mg) and acid-extractable metal content (0.9-16 mg/mg). Intratracheal exposure to all five particles induced similar toxicological responses, with different potency. Lung particle retention was observed in DEP13 and HVO13 exposed mice on day 28 post-exposure, with less retention for the other fuel types. RME exposure induced limited response whereas the remaining particles induced dose-dependent inflammation and acute phase response on day 1. DEP13 induced acute phase response on day 28 and inflammation on day 90. DNA strand break levels were not increased as compared to vehicle, but were increased in lung and liver compared to blank filter extraction control. Neutrophil influx on day 1 correlated best with estimated deposited surface area, but also with elemental carbon, organic carbon and PAHs. DNA strand break levels in lung on day 28 and in liver on day 90 correlated with acellular particle-induced ROS. Conclusions We studied diesel exhaust particles designed to differ in physicochemical properties. Our study highlights specific surface area, elemental carbon content, PAHs and ROS-generating potential as physicochemical predictors of diesel particle toxicity.

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Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans

et al. 2021

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Inflammation, oxidative stress and genotoxicity responses to biodiesel emissions in cultured mammalian cells and animals

Cited by 31 publications

References 130 publications

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans

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