Inflammation-Related MicroRNAs Are Associated with Plaque Stability Calculated by IVUS in Coronary Heart Disease Patients

Abstract

Objectives This study aimed to investigate the association between inflammation-related microRNAs (miR-21, 146a, 155) and the plaque stability in coronary artery disease patients. Methods The expression of miR-21, 146a, and 155 was measured by real-time PCR in 310 consecutive patients. The level of hs-CRP, IL-6, and IL-8 was measured by ELISA. The plaque stability of coronary stenotic lesions was evaluated with intravascular ultrasound (IVUS). Results (1) The levels of hs-CRP, IL-6, and IL-8 were significantly increased in the UAP and AMI groups compared with the CPS group (P < 0.01). (2) The expression of miR-21 and miR-146a in peripheral blood mononuclear cells (PBMCs) and plasma was significantly higher in CAD patients compared with non-CAD patients, whereas the miR-155 expression in PBMCs and plasma was significantly lower in patients with CAD. (3) The miR-21 expression in PBMCs was higher in UAP and AMI groups compared with CPS group. The miR-146a expression in PBMCs was higher in SAP, UAP, and AMI groups than in CPS group. Although the level of miR-155 in PBMCs was lower in SAP, UAP, and AMI groups than in CPS group. The expression patterns of miR-21, miR-146a, and miR-155 in plasma were consistent with those of PBMCs. (4) The expressions of miR-21 and miR-146a in PBMCs and plasma were significantly higher in the vulnerable plaque group than those in stable plaque group. While miR-155 in PBMCs and plasma was significantly lower in vulnerable plaque group compared with stable plaque group. (5) The levels of miR-21 and miR-146a in PBMCs and plasma were significantly higher in soft plaque group than in fibrous plaque group and calcified plaque group. However, miR-155 in PBMCs and plasma was significantly lower in soft plaque group. Conclusions The expression of miR-21 and miR-146a are associated with the plaque stability in coronary stenotic lesions, whereas miR-155 expression is inversely associated with the plaque stability.

Puerarin alleviates cisplatin‑induced acute renal damage and upregulates microRNA‑31‑related signaling

“…A previous study reported that puerarin facilitated the proliferation, migration and invasion of colorectal cancer cells by targeting Numb protein (18). Numb was originally identified as an intrinsic determinant in Drosophila, affecting the cell fate by negatively altering the Notch signaling pathway (17). Numb is predominantly expressed in normal proximal tubules and detected in interstitial cells of fibrotic kidneys (31).…”

“…miR-31 was also found to directly target the 3'-untranslated region (UTR) sequence of the Numb gene (16). Furthermore, miR-31-5p was reported to promote the proliferation of colorectal cancer cells by targeting the Numb protein (17). Numb is considered an intrinsic determinant in Drosophila, which alters the cell fate by negatively regulating the Notch signaling pathway (18).…”

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“…60 Interestingly, a study associated miR-146a levels with plaque stability in coronary stenotic lesions. 61 The authors found raised miR-146a levels in human peripheral blood mononuclear cells (PBMCs) from vulnerable plaque group versus those from stable plaque group. 61 Accordingly, miR-146a was also increased in PBMCs samples from CAD patients.…”

Emerging Challenges of Radiation-Associated Cardiovascular Dysfunction (RACVD) in Modern Radiation Oncology: Clinical Practice, Bench Investigation, and Multidisciplinary Care

“…In the literature, many clinical studies assessed circulating miRNA levels in peripheral blood for diagnosing, predicting, and monitoring human diseases (158)(159)(160)(161)(162)(163), including cardiac and vascular disease (CVD) (164)(165)(166)(167)(168)(169). For example, the combination of miR-34a-5p and fibrinogen levels have been reported as a useful tool in differentiating pre-thrombotic status in patients with stable coronary artery disease (165).…”