Inflammation-related proteomics demonstrate landscape of fracture blister fluid in patients with acute compartment syndrome

Abstract

BackgroundBlisters are tense vesicles or bullae that arise on swollen skin and are found in a wide range of injuries. As a complication of fracture, fracture blisters are considered soft tissue injuries, which often lead to adverse effects such as prolonged preoperative waiting time and increased risk of surgical site infection. However, our previous study found that in patients with acute compartment syndrome, fracture blisters may be a form of compartment pressure release, but the specific mechanism has not been revealed. Here, we mapped out the proteomic landscape of fracture blister fluid for the first time and compared its expression profile to cupping and burn blisters.MethodsFirst, fluid samples were collected from 15 patients with fracture blisters, 7 patients with cupping blisters, and 9 patients with burn blisters. Then, the expression levels of 92 inflammatory proteins were measured using the Olink Target 96 Inflammation panel. Protein profiles were compared across the three groups using Differential Protein Expression Analysis and Principal Component Analysis (PCA).ResultsFracture blisters had significantly higher levels of 50 proteins in comparison to cupping and 26 proteins in comparison to burn blisters. Notably, PCA showed fracture blisters closely resembled the protein expression profile of burn blisters but were distinct from the protein expression profile of cupping blisters.ConclusionOur study provides the first characterization of fracture blister fluid using proteomics, which provides a valuable reference for further analysis of the difference between blisters caused by fractures and those caused by other pathogenic factors. This compendium of proteomic data provides valuable insights and a rich resource to better understand fracture blisters.

Olink proteomics analysis uncovers the landscape of inflammation-related proteins in patients with acute compartment syndrome

“…In our previous research, complete blood counts revealed that the levels of NEU and CKMB were related to ACS, but a limited number of inflammation-related indicators were observed. Our recent studies also found some variations of inflammatory cytokines in patients with fracture blister fluid and plasma through Olink Proteomics analysis (11,12). Therefore, we used the inflammatory panel of Olink Proteomics analysis to identify the biomarkers of ACS in the current study.…”

Vitreous Olink proteomics reveals inflammatory biomarkers for diagnosis and prognosis of traumatic proliferative vitreoretinopathy

“…Vitreous samples were measured using the Olink ® INFLAMMATION panel* (Olink Proteomics AB, Uppsala, Sweden) according to the manufacturer’s instructions, which allows the simultaneous analysis of 92 analytes using 1 µL of each sample ( 13 , 14 , 16 ). Detailed information about the panel can be found in the Supplementary files , Supplementary Data Sheet 1 .…”