Inflammatory bowel disease — is there something new in the immunological background?

Abstract

Abstract: Abstract: Abstract: In the present paper we correlate clinical data, as well as histopathological, immunohistochemical and molecular biology methods, with the occurrence of both forms of inflammatory bowel disease (IBD) i.e. ulcerative colitis and Crohn's disease. We found that patients with a history of Epstein-Barr virus (EBV) or cytomegalovirus (CMV) infections, as well as steroid treatment, had increased susceptibility to the development of IBD. The diagnosis of IBD was confirmed by histopathology. Previous infections by EBV and CMV, as well as M. tuberculosis, were proved by PCR-based techniques and in situ hybridization. We found PCR-proved latent viral infections in 30-50% of the IBD patients we studied. However, we were unable to prove the presence of viral antigens by immunohistochemistry for EBV or CMV. We found positive correlations between the presence of anti-CMV IgG, as well as PCR-positive results for M. tuberculosis with an ulcerative colitis diagnosis. Additionally, up to 80% of IBD patients used steroids, which was found to be correlated with a diagnosis of Crohn's disease. Our data may support the theory that IBD could be related to previous viral infections and the use of

Current knowledge and systematic review of viruses associated with Crohn's disease

“…Using three different ISH probes, Gerhert and colleagues detected EBV in inflamed tissue sections from both CD and UC patients at a higher rate compared with tissue sections without inflammation, suggesting that the virus was associated with active inflammation . In 2011, similar detection rate of EBV in gut tissue from CD and controls was reported .…”

Epstein-Barr Virus Infection Is Common in Inflamed Gastrointestinal Mucosa

“…[43–50] Two groups describe finding PCR-amplifiable EBV DNA in inflammatory bowel disease tissues. [44,48] Yanai et al localized EBER expression to non-epithelial cells (B lymphocytes and histiocytic cells) in both Crohn’s disease and ulcerative colitis tissues. [47] Gehlert et al described preferential localization of EBER -expressing cells to zones of active inflammation in both diseases.…”

Ureter Regeneration–The Proper Scaffold Has to Be Defined

“…This analysis was performed according to the procedure described previously [27]. Briefly, tissue sections were incubated with primary antibody against α-SMM (dilution 1∶400).…”