Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus

Méndez‐Flores, Silvia; Hernández-Molina, Gabriela; Azamar-Llamas, Daniel; Zúñiga, Joaquı́n; Romero-Díaz, Juanita; Furuzawa‐Carballeda, Janette

doi:10.1016/j.cyto.2019.03.010

Cited by 23 publications

(28 citation statements)

References 39 publications

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“…Our study did not show significant differences in RANTES and IP10 concentration between the groups studied. However, it was shown that chemokines play a role in many inflammatory diseases such as systemic lupus erythematosus, Kawasaki disease, Wegener’s granulomatosis, and Behcet’s disease [ 40 – 42 ]. IP10 is a strong attractant for macrophages and NK cells.…”

Section: Discussionmentioning

confidence: 99%

Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in children

et al. 2019

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Section: Discussionmentioning

confidence: 99%

Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in children

et al. 2019

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“…Immunohistochemistry. We followed the methods of Méndez-Flores et al [26]. Briefly, IL-22-expressing cells were determined in 4 μm thick sections of tissue.…”

Section: Skin Samples Skin Punch Biopsies (4 MM Diameter)mentioning

confidence: 99%

“…The reactive blank was incubated with phosphate buffer saline-egg albumin (Sigma-Aldrich) instead of the primary antibody. Both controls excluded nonspecific staining or endogenous enzymatic activities [26]. Spleen and ganglion samples were used as a positive control ( Figure S1).…”

Section: Skin Samples Skin Punch Biopsies (4 MM Diameter)mentioning

confidence: 99%

“…Double-Staining Procedure. We followed the methods of Méndez-Flores et al [26]. To determine the subpopulation of CD4 + /IL-17A + -, CD4 + /IL-4 + -, CD4 + /IFN-γ + -expressing T cells, CD25 + /Foxp3 + regulatory T cells, CD20 + /IL-10 + -producing B cells, and CD123 + /IDO + pDC cell subpopulations, a simultaneous detection was performed (MultiView (mouse-HRP/rabbit-AP) Enzo Life Sciences PBMCs were incubated with 5 μl of Human TruStain FcX™ (BioLegend Inc.) per million cells in 100 ml PBS for 10 minutes, and then, they were labeled with 5 μl of antihuman CD3-FITC-labeled, antihuman CD4-PeCy5-labeled, and antihuman CD161-APC-conjugated monoclonal antibodies (BD Biosciences, San Jose, CA); antihuman CD3-FITC-labeled, antihuman CD4-PeCy5-labeled, and antihu-man CD25-APC-conjugated monoclonal antibodies (BD Biosciences); antihuman CD19-APC-labeled, antihuman CD24-FITC-conjugated, and antihuman CD38-PeCy5labeled monoclonal antibodies (BD Biosciences); or antihuman CCR6-PerCP/Cy5.5-conjugated and antihuman CD123-FITC-labeled monoclonal antibodies (BD Biosciences) in separated tubes during 20 min at 37°C in the dark.…”

Section: Skin Samples Skin Punch Biopsies (4 MM Diameter)mentioning

confidence: 99%

“…Finally, T subsets were analyzed by flow cytometry with an Accuri C6 (BD Biosciences). A total of 500,000-1,000,000 events were recorded for each sample and analyzed with the FlowJo X software (Tree Star, Inc.) [26].…”

Section: Skin Samples Skin Punch Biopsies (4 MM Diameter)mentioning

confidence: 99%

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MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis

Méndez‐Flores

Furuzawa‐Carballeda

Hernández-Molina

et al. 2019

Mediators of Inflammation

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Background. The role of miRNAs in the pathogenesis of cutaneous lupus has not been studied. Objective. It was to assess the levels of a selected panel of circulating miRNAs that could be involved in the regulation of the immune response, inflammation, and fibrosis in cutaneous lupus. Methods. It was a cross-sectional study. We included 22 patients with subacute (SCLE) and 20 with discoid (DLE) lesions, and 19 healthy donors (HD). qRT-PCR for miRNA analysis, flow cytometry in peripheral blood, and skin immunohistochemistry were performed to determine the distribution of CD4 T cells and regulatory cells and their correlation with circulating miRNAs. Results. miR-150, miR-1246, miR-21, miR-23b, and miR-146 levels were downregulated in SCLE vs. HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE vs. HD. Peripheral CD4+/CD25-/IL-4+ cells and CD4+/CD25hi/Foxp3+ were negatively associated with miR-23b, and CD4+/CD25-/IFN-γ+ with miR-1246 in SCLE, whereas CD123+/CD196+/IDO+ cells were positively associated with miR-150 in DLE. In the tissue, CD4+/IL-4+ and CD20+/IL-10+ cells were positively associated with miR-21 and CD4+/IFN-γ+ with miR-31 in SCLE, whereas CD4+/IL-4+ cells were positively associated with miR-150, and CD20+/IL-10+ cells with miR-1246 and miR-146a in DLE. In the SCLE, lower miR-150 levels were correlated with higher CLASI scores. The KEGG pathway enrichment analysis revealed that cell cycle regulation pathways, p53, TGF-β, thyroid hormone, and cancer signaling pathways were shared between miR-21, miR-31, miR-23b, miR-146a, miR-1246, and miR-150. Conclusions. A downregulation of miR-150, miR-1246, and miR-21 in both CLE varieties vs. HD was determined.

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Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy

Chen

Zhang²,

Li³

et al. 2022

Immunity Inflam & Disease

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Background This study used astragalus membranaceus (AM) to treat systemic lupus erythematosus (SLE) model mice during pregnancy, aiming to explore the role of AM in Helper T cell 17 (Th17) differentiation and SLE during pregnancy. Methods We used lipopolysaccharide to constructed the SLE mouse model. AM decoction given by intragastric administration lasted from the eighth week of the mouse age until the mouse was killed. We estimated the messenger RNA levels of IL‐17a and Rorc, counted the Th17 cell number and examined the levels of cytokines including interleukin (IL)‐12, tumor necrosis factor α, interferon gamma, IL‐17A in mouse serum. Periodic acid‐Schiff staining and renal glomerular/tubulointerstitial (TI) score were used to evaluate the progression of lupus nephritis (LN). Results AM treatment improved the conception rate and increased the number and average weight of fetuses in SLE mice. It significantly decreased the urinary albumin/creatinine ratios and reduced the glomerular scores and TI scores in the pregnant SLE mice. AM gavage significantly decreased the weight of spleen, mesenteric lymph node, total splenocytes and T cells, and the expression of proinflammatory factors. Furthermore, AM treatment reduced the ratio of Th17 cells and the expression levels of RORγt and IL‐17A. Conclusion AM significantly improved pregnancy outcomes and inhibited lupus nephritis during pregnancy in SLE mice.

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Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus

Cited by 23 publications

References 39 publications

Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in children

Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in children

MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis

Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy

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