Inflammatory Cytokines and BDNF Response to High-Intensity Intermittent Exercise: Effect the Exercise Volume

Abstract

The purpose of this study was to compare the effects of two similar high-intensity intermittent exercises (HIIE) but different volume 1.25 km (HIIE1.25) and 2.5 km (HIIE2.5) on inflammatory and BDNF responses. Ten physically active male subjects (age 25.22 ± 1.74 years, body mass 78.98 ± 7.31 kg, height 1.78 ± 0.06 m, VO2peak 59.94 ± 9.38 ml·kg·min−1) performed an incremental treadmill exercise test and randomly completed two sessions of HIIE on a treadmill (1:1 min at vVO2max with passive recovery). Blood samples were collected at rest, immediately and 60-min after the exercise sessions. Serum was analyzed for glucose, lactate, IL-6, IL-10, and BDNF levels. Blood lactate concentrations was higher immediately post-exercise compared to rest (HIIE1.25: 1.69 ± 0.26–7.78 ± 2.09 mmol·L−1, and HIIE2.5: 1.89 ± 0.26–7.38 ± 2.57 mmol·L−1, p < 0.0001). Glucose concentrations did not present changes under the different conditions, however, levels were higher 60-min post-exercise than at rest only in the HIIE1.25 condition (rest: 76.80 ± 11.14–97.84 ± 24.87 mg·dL−1, p < 0.05). BDNF level increased immediately after exercise in both protocols (HIIE1.25: 9.71 ± 306–17.86 ± 8.59 ng.mL−1, and HIIE2.5: 11.83 ± 5.82–22.84 ± 10.30 ng.mL−1). Although both exercises increased IL-6, level percent between rest and immediately after exercise was higher in the HIIE2.5 than HIIE1.25 (30 and 10%; p = 0.014, respectively). Moreover, IL-10 levels percent increase between immediately and 60-min post-exercise was higher in HIIE2.5 than HIIE1.25 (37 and 10%; p = 0.012, respectively). In conclusion, both HIIE protocols with the same intensity were effective to increase BDNF and IL-6 levels immediately after exercise while only IL-10 response was related to the durantion of exercise indicanting the importance of this exercise prescription variable.

Interleukin‐10 responses from acute exercise in healthy subjects: A systematic review

“…Regardless of, levels of IL‐10 tend to peak at the cessation of the exercise or shortly thereafter. One previous study shows that after acute exercise (5‐km continuous run on a treadmill at 70% of sVO 2 max) peak concentrations of IL‐10 were reached 60 min after the cessation of acute exercise compared with baseline levels (Cabral‐Santos et al, ). Thus, the systemic effects induced by IL‐10 are for the most part perceived to occur during recovery time from exercise.…”

“…In response to amount of time, prior research has showed that in high‐intensity intermittent exercises of different exercise volumes (running 1.25 and 2.5 km with 1:1 min at sVO 2 max) the increase in IL‐10 concentration was related to the duration of exercise indicating the importance of this variable (Cabral‐Santos et al, ). Taken together, these results showed that changes in IL‐10 production are remarkably related to the duration of exercise.…”

Comparison of the inflammatory and stress response between sprint interval swimming and running

“…It should be highlighted that contracting muscles secrete IL‐6, stimulating the appearance in the circulation of the anti‐inflammatory cytokine IL‐10, which directly inhibits TNF‐α transcription . However, IL‐10 release into the circulation is not a consistent finding in response to high‐intensity exercise protocols . Our results suggest that “all out” sprint exercise is a good time‐efficient strategy for promoting an anti‐inflammatory response.…”

“…11 However, IL-10 release into the circulation is not a consistent finding in response to high-intensity exercise protocols. 14,27,28 Our results suggest that "all out" sprint exercise is a good time-efficient strategy for promoting an anti-inflammatory response.…”

The Effect of a 12-Week Health Training Program on Selected Anthropometric and Biochemical Variables in Middle-Aged Women

“…A lot of research was performed on the kinetics of changes in cytokine levels following acute exercise [ 44 , 46 , 47 ] and regular physical activity [ 48 , 49 ]. The effects of gender [ 46 ], training loads [ 50 ], and age [ 48 , 49 ] on the kinetics of posttraining cytokine levels were also analyzed.…”