Inflammatory markers in ewes submitted to surgical or transcervical embryo collection

Oliveira, Fernando Caetano de; Haas, Cristina; Ferreira, C. E. R.; Goularte, Karina Lemos; Pegoraro, L. M. C.; Gasperin, Bernardo Garziera; Schneider, Augusto; Mondadori, Rafael Gianella; Lucia, Thomaz; Vieira, Arnaldo Diniz

doi:10.1016/j.smallrumres.2017.11.012

Cited by 13 publications

(10 citation statements)

References 15 publications

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“…The duration of embryo flushing approached to 25 min in both treatment groups. This time is similar to 30 min reported in Dorper sheep, also receiving epidural anaesthesia (Gusmão et al., ), or cervical (26 min) and laparoscopic (30 min) embryo recovery in Crioula wool sheep (Oliveira et al., ). In addition, the overall flushing fluid recovery (~93%) appeared to be satisfactory, and it was similar to the 95% recovery rate reported by Gusmão et al.…”

Section: Discussionsupporting

confidence: 84%

Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Fonseca

Zambrini

Guimarães

et al. 2018

Reprod Domestic Animals

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This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7-day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d-cloprostenol by latero-vulvar route 7 days apart. After the second injection of d-cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d-cloprostenol (latero-vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d-cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous-synchronized Santa Inês ewes pretreated with d-cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.

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Section: Discussionsupporting

confidence: 84%

Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Fonseca

Zambrini

Guimarães

et al. 2018

Reprod Domestic Animals

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“…In this sense, Leite et al () have demonstrated advances in hormonal protocols for cervical dilation based on the combination of oestradiol and oxytocin previously to the TC method. Moreover, according to Oliveira et al (), the TC method is less invasive, allows animals to be used successively and has no postsurgical effects such as adherence with consequent fertility impairment. Therefore, this technique could be a viable alternative for embryo collection in sheep MOET programmes.…”

Section: Introductionmentioning

confidence: 99%

Cervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection

Santos

Arashiro

Balaro

et al. 2018

Reprod Domestic Animals

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This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (κ = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route.

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“…Comparisons between surgical and transcervical techniques demonstrate that both are efficient in embryo recovery (Santos et al, 2020), with similar (Oliveira et al, 2018) or even reduced Santos et al, 2020) durations of execution and expressing the same inflammatory markers (Oliveira et al, 2018). NSER is more advantageous as it is a less invasive and simplified approach.…”

Section: New Approaches and Perspectivesmentioning

confidence: 99%

“…Cervix manipulation causes a transient increase in inflammatory markers (Santos et al, 2020), but this does not seem to affect reproduction in the short term as the return to estrus and fertilization of Dorper ewes after NSER has already been observed . Nonetheless, Oliveira et al (2018) suggest that studies evaluating the repeatability of the NSER technique should be done in sheep.…”

Section: New Approaches and Perspectivesmentioning

confidence: 99%

“…The causes of ERCL are not fully elucidated, but are related to a drop in P4 concentrations (SAHARREA et al, 1998) and inadequate follicle luteinization, leading to a consistently high estradiol (E2) concentration (OKADA et al, 2000). It is more frequent in superovulated animals, probably due to the greater number of developing follicles , affecting part or all CLs (Oliveira et al, 2018). For SOV programs, the ERCL is a reason of concern, since it has as a consequence an abnormal ova transport and recovery of poorer quality embryos (Ishwar and Memon, 1996a;.…”

Section: Introductionmentioning

confidence: 99%

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Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

Dias¹

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Multiple ovulation and embryo transfer (MOET) play a key role in accelerating sheep farming, but it is still limited by obstacles such as embryo recovery technique and variability in the superovulatory (SOV) response. This study evaluates alternatives to these limitations, aiming to increase in vivo embryo production in sheep. The first chapter reviews some relevant points of non-surgical embryo recovery (NSER): ovine cervical anatomy, physiological mechanisms of cervical relaxation, and current cervical relaxation protocols (PRC). The second and third chapters evaluate the efficiency of PRC using zero (0.0mg), half (0.5mg), or full dose (1.0mg) of estradiol benzoate (EB) in synchronized and/or superovulated ewes and its effects on NSER feasibility and luteal function. Sheep were randomly assigned to receive 37.5 µg of d-cloprostenol i.m. and 0.0 mg (0EB group; n=12); 0.5 mg (0.5EB group; n=12) or 1.0 mg EB (1.0EB group, n=12) 16 h before and 50 IU oxytocin i.v. 20 minutes before the NSER. In synchronized sheep, NSER was performed in 91.7% (33/36) with a longer transposition duration with Hegar dilator and longer catheter-mandrel (P<0.05) in 0.0EB. The treatments did not differ (P>0.05) in the duration of uterine flushing or the rate of structure recovery. In superovulated ewes, there was no treatment or category effect (P>0.05) concerning NSER success. There was a reduction (P<0.05) in ovarian biometry and progesterone (P4) concentration and an increase in estradiol (E2) concentration on D17. The fourth chapter assesses the effectiveness of hCG administration after ovulation. Ovarian function, P4 profile, and embryo yield were evaluated. Superovulated ewes received 300 IU of hCG i.m. (GhCG, n=24) or not (GControl, n=25) 96 h after P4 removal. NSER efficiency, SOV response, and luteal tissue area were similar between groups (P>0.05). The hCG increased (P<0.05) P4 levels and tended to prevent structural luteolysis (P=0.07) but had no effect (P>0.05) on functional luteolysis. The fifth chapter studies the effect of 250, 333, or 400 IU of a commercial pFSH (1FSH: 1LH) on follicular growth, SOV response, and embryo yield. Superovulated ewes with decreasing doses of 250 (G250), 333 (G333), or 400 IU (G400) of pFSH were submitted to NSER. Estrus response, estrus interval, CL and LUF counts and follicular growth did not differ (P>0.05) between groups. The treatments did not differ (P>0.05) in embryo yield, but G333 and G400 showed a tendency (P=0.06) to have a higher mean of recovered structures compared to G250. This study concludes that: 1) NSER can be successfully performed in different sheep breeds, regardless of category, using a cervical relaxation protocol without estradiol benzoate; 2) the use or not of EB affected the luteal function, interfering mainly in estradiol levels; 3) hCG treatment increased circulating P4 before NSER and may prevent early luteal regression; 4) protocols using 250, 333 and 400 IU of pFSH are efficient in promoting follicular growth and superovulatory responses, but 333 IU appears to be more efficient in increasing embryo yield. Keywords: Biotechnologies. Embryos. MOET. Transcervical.

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Inflammatory markers in ewes submitted to surgical or transcervical embryo collection

Cited by 13 publications

References 15 publications

Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Cervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection

Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

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