Inflammatory markers in exhaled breath condensate from patients with asthma

Ueno, Tomoe; Kataoka, Masao; Hirano, Atsushi; Iio, Kouji; Tanimoto, Yasushi; Kanehiro, Arihiko; Okada, Chiharu; Soda, Ryo; Takahashi, Kiyoshi; Tanimoto, Mitsune

doi:10.1111/j.1440-1843.2008.01315.x

Cited by 53 publications

(73 citation statements)

References 34 publications

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“…Contrary to our results, other studies have reported total NO 2 -/NO 3 -level in EBC to be elevated in subjects with asthma compared to healthy nonsmoking subjects [17], healthy nonatopic controls [5] or controls [9]. These conflicting results may be due to the very small number of subjects included in these studies, the various methodologies used for measuring NO 2 -and NO 3 -levels, the choice of the reference group for comparisons and other differences such as those related to phenotypes definition.…”

Section: Discussioncontrasting

confidence: 99%

“…Both epidemiological and clinical studies have shown increased level of FeNO in children and adults with asthma, and positive associations between FeNO and allergic sensitisation are consistent over the studies, regardless of rhinitis or asthma [4]. Studies on associations between NO 2 -and NO 3 -levels with asthma, allergy or rhinitis have led to more conflicting results both in adults and children [5][6][7][8][9][10][11][12][13][14][15][16][17]. Until now, none of these studies has simultaneously performed measurements of EBC NO 2 -/NO 3 -and FeNO levels in the same subjects.…”

Section: Introductionmentioning

confidence: 87%

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Exhaled nitric oxide, nitrite/nitrate levels, allergy, rhinitis and asthma in the EGEA study

et al. 2014

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Although interest in biomarkers in the nitrate-nitrite-NO pathway has recently increased, associations between nitrite (NO 2 -) and nitrate (NO 3 -), and asthma, allergic sensitisation and rhinitis remain unclear. The study aimed to evaluate the associations between NO 2 -/NO 3 -and exhaled fraction of nitric oxide (FeNO) levels with asthma, allergic sensitisation and rhinitis.Plasma and exhaled breath condensate (EBC) NO 2 -/NO 3 -and FeNO levels were measured in 523 adults of the French Epidemiological study on Genetics and Environment of Asthma. Allergic sensitisation was defined by a positive skin prick test for at least one aeroallergen. Subjects were classified as non-sensitised, sensitised and as having allergic rhinitis.Plasma NO 2 -/NO 3 -level was unrelated to any disease phenotypes. EBC NO 2 -/NO 3 -level was unrelated to any asthma phenotypes. EBC NO 2 -/NO 3 -and FeNO levels were correlated in sensitised subjects only (r50.21¡0.10, p50.01). EBC NO 2 -/NO 3 -and FeNO levels were higher in sensitised than in non-sensitised subjects (adjusted geometric mean (95% CI): 2.36 (1.96-2.84) versus 1.72 (1.38-2.14) mmol per mg proteins, p50.008; and 18.3 (16.7-20.0) versus 14.8 (13.3-16.5) ppb, p50.0006, respectively), with gradual relationships from sensitised subjects to those with allergic rhinitis (p,0.0001).Results suggest that EBC NO 2 -/NO 3 -and FeNO levels may be considered as biological markers of intensity of allergic sensitisation and rhinitis. @ERSpublications The EGEA study: exhaled nitrate/nitrite/NO levels may be considered markers for intensity of allergy and rhinitis

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Exhaled nitric oxide, nitrite/nitrate levels, allergy, rhinitis and asthma in the EGEA study

et al. 2014

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“…In the study performed by Ueno et al [31], in which CRP was measured using ELISA with a high sensitivity, the authors demonstrated raised CRP levels in the EBC in asthmatics compared with healthy volunteers. These results were confirmed by our previous report, in which the increase in hs-CRP levels both in serum and EBC and the correlation of this parameter with F ENO and ECP in asthmatic patients were revealed [32].…”

Section: Discussionmentioning

confidence: 99%

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Ziętkowski¹,

Skiepko²,

Tomasiak-Lozowska³

et al. 2010

Int Arch Allergy Immunol

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Background: Exercise-induced bronchoconstriction (EIB) in asthmatics depends on the presence of allergic inflammation. This study was performed to assess the possible association of EIB with low-grade systemic inflammation, whose presence was revealed in asthmatic patients. Methods: The study was conducted in a group of 24 asthmatics (14 with EIB, 10 without EIB) and 8 healthy volunteers. Changes in serum and exhaled breath condensate (EBC) high-sensitivity C-reactive protein (hs-CRP) levels induced by intensive exercise were determined. Moreover, the possible correlation of these measurements with the results of other tests used in the diagnosis of asthma as well as laboratory tests commonly associated with asthma were investigated. Results: In asthmatic patients with EIB, a statistically significant increase in hs-CRP levels both in serum and EBC after an exercise test was observed. Twenty-four hours after the exercise test in the group of asthmatics with EIB, a statistically significant increase in exhaled nitric oxide (F_ENO), serum eosinophil cationic protein (ECP) concentrations and bronchial hyperreactivity to histamine was revealed. A statistically significant correlation between the maximum increase in hs-CRP levels both in serum and EBC after exercise and either baseline F_ENO and an increase in serum ECP or F_ENO 24 h after exercise in the group of asthmatics with EIB was revealed. Conclusions: We show that, as a result of intensive exercise leading to bronchoconstriction, an increase in serum and EBC hs-CRP occurs. Our observations could suggest that in asthmatic patients, as a consequence of exercise-induced bronchoconstriction, an intensification of low-grade systemic inflammation can be observed.

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“…Both H 2 O 2 and NO are associated with oxidative stress, which is defined as an imbalance between oxidants and antioxidants, and is thought to be involved in airway inflammation and respiratory diseases [47] H 2 O 2 is generated from superoxide anion by the action of superoxide dismutase in neutrophils, eosinophils, macrophages and epithelial cells, and in particular, by eosinophils in asthma [48][49][50] Exhaled nitric oxide (eNO) is a marker of airway inflammation [1]. Although NO is produced in the airways by a variety of cells, large amounts may be generated from those involved in the acute and chronic inflammatory responses [51].…”

Section: Article In Pressmentioning

confidence: 99%

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

Stefańska

Sokołowska

Sarniak

et al. 2010

Pulmonary Pharmacology & Therapeutics

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ARTICLE IN PRESS AbstractThe imbalance between reactive oxygen species (ROS) synthesis and antioxidants might be involved in the pathogenesis of many inflammatory diseases. NADPH oxidase, an enzyme responsible for ROS production, may represent an attractive therapeutic target to inhibit for the treatment of these diseases.Apocynin is an inhibitor of activation of NADPH oxidase complex present in the inflammatory cells.In double blind, placebo controlled, cross-over study, we investigated the effect of nebulized apocynin on ROS synthesis in 10 nonsmoking healthy volunteers. Apocynin (6 ml of 0.5 mg/ml) was administered by nebulization and its effects on H 2 O 2 , NO 2 -and NO 3 -generation were assessed after 30, 60 and 120 minutes by collecting exhaled breath condensate (EBC) samples using an EcoScreen analyzer. Additionally, respiratory parameters have been evaluated, utilizing spirometry and DLCO. We also analyzed peripheral blood differential counts and NO 2 -serum level, cough scale control and blood pressure as safety parameters. Thus, as apocynin significantly influence ROS concentration, it might have also antiinflammatory properties. As it is safe, it may have a potential to become a drug in airway inflammatory diseases treatment.

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Inflammatory markers in exhaled breath condensate from patients with asthma

Abstract: Measurement of EBC biomarkers is a non-invasive and useful way to evaluate airway inflammation in patients with asthma.

Cited by 53 publications

References 34 publications

Exhaled nitric oxide, nitrite/nitrate levels, allergy, rhinitis and asthma in the EGEA study

Exhaled nitric oxide, nitrite/nitrate levels, allergy, rhinitis and asthma in the EGEA study

Changes in High-Sensitivity C-Reactive Protein in Serum and Exhaled Breath Condensate after Intensive Exercise in Patients with Allergic Asthma

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

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