Inflammatory markers in saliva for diagnosis of sepsis of hospitalizes patients

Galhardo, Luciana Freitas; Ruivo, Gilson Fernandes; Oliveira, Luciane Dias de; Parize, Graziele; Santos, Silvana Soléo Ferreira dos; Pallos, Débora; Leão, Mariella Vieira Pereira

doi:10.1111/eci.13219

Cited by 20 publications

(19 citation statements)

References 16 publications

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“…Such information has been suggested to be useful for the diagnosis and prognosis of both oral cavity and systemic diseases. 13 Hence, it is possible to establish an inflammatory profile of COVID-19 by analysing inflammation-related biomarkers in saliva.…”

Section: Saliva Biomarkers-perspectives For Covid-19 Diagnosis and Prmentioning

confidence: 99%

COVID-19 salivary signature: diagnostic and research opportunities

et al. 2020

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The COVID-19 (caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) epidemic started in Wuhan (Hubei Province, China) in mid-December 2019 and quickly spread across the world as a pandemic. As a key to tracing the disease and to implement strategies aimed at breaking the chain of disease transmission, extensive testing for SARS-CoV-2 was suggested. Although nasopharyngeal/oropharyngeal swabs are the most commonly used biological samples for SARS-CoV-2 diagnosis, they have a number of limitations related to sample collection and healthcare personnel safety. In this context, saliva is emerging as a promising alternative to nasopharyngeal/oropharyngeal swabs for COVID-19 diagnosis and monitoring. Saliva collection, being a non-invasive approach with possibility for self-collection, circumvents to a great extent the limitations associated with the use of nasopharyngeal/oropharyngeal swabs. In addition, various salivary biomarkers including the salivary metabolomics offer a high promise to be useful for better understanding of COVID-19 and possibly in the identification of patients with various degrees of severity, including asymptomatic carriers. This review summarises the clinical and scientific basis for the potential use of saliva for COVID-19 diagnosis and disease monitoring. Additionally, we discuss saliva-based biomarkers and their potential clinical and research applications related to COVID-19.

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Section: Saliva Biomarkers-perspectives For Covid-19 Diagnosis and Prmentioning

confidence: 99%

COVID-19 salivary signature: diagnostic and research opportunities

et al. 2020

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“…The human saliva has hundreds of microbial species, many of which are related to diseases affecting humans [ 3 ], including hereditary diseases, autoimmune diseases, malignancies, and infections [ 4 , 5 ]. Saliva has also been used as an important diagnostic tool in previous coronavirus infections like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of RNA Extraction-Free Method for Detection of SARS-CoV-2 in Salivary Samples for Mass Screening for COVID-19

Mahmoud

Ganesan²,

Ibrahim

et al. 2021

BioMed Research International

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In this COVID-19 pandemic, there is a dire need for cost-effective and less time-consuming alternatives for SARS-CoV-2 testing. The RNA extraction-free method for detecting SARS-CoV-2 in saliva is a promising option. This study found that it has high sensitivity (85.34%), specificity (95.04%), and was comparable to the gold standard nasopharyngeal swab (NPS) sample tests. The method showed good agreement between salivary and NPS samples, with a kappa coefficient of 0.797. However, there are variations in the sensitivity and specificity based on the RT-PCR kit used. The Thermo Fisher Applied Biosystems showed high sensitivity, positive predictive value (PPV), and negative predictive value (NPV) but also showed a higher percentage of invalid reports. On the other hand, the BGI kit showed high specificity, better agreement (kappa coefficient) between the results of saliva and NPS samples, and higher correlation between the Ct values of saliva and NPS samples. Thus, the RNA extraction-free method for salivary sample serves as an effective alternative screening method for COVID-19.

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“…The human saliva has hundreds of microbial species, many of which are related to diseases affecting humans [3],including hereditary, autoimmune diseases, malignancies and infections [4,5]. Saliva has also been used as an important diagnostic tool in previous coronavirus infections like severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of RNA extraction free method for detection of SARS-COV-2 in salivary samples for mass screening for COVID-19

Mahmoud

Ibrahim

Ganesan³

et al. 2021

Preprint

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In this current COVID 19 pandemic, there is a dire need for cost effective and less time-consuming alternatives for SARS-COV-2 testing. The RNA extraction free method for detecting SARS COV 2 in saliva is a promising option, this study found that it has high sensitivity (85.34%), specificity (95.04%) and was comparable to the gold standard nasopharyngeal swab. The method showed good percentage of agreement (kappa coefficient) 0.797 between salivary and NPS samples. However, there are variations in the sensitivity and specificity based on the RT-PCR kit used. The Thermo Fischer- Applied biosystems showed high sensitivity, PPV and NPV but also showed higher percentage of invalid reports. Whereas the BGI kit showed high specificity, better agreement (kappa coefficient) between the results of saliva and NPS samples and higher correlation between the Ct values of saliva and NPS samples. Thus, the RNA extraction free method for salivary sample serves as an effective alternative for SARS CoV 2 testing.

show abstract

Inflammatory markers in saliva for diagnosis of sepsis of hospitalizes patients

Cited by 20 publications

References 16 publications

COVID-19 salivary signature: diagnostic and research opportunities

COVID-19 salivary signature: diagnostic and research opportunities

Evaluation of RNA Extraction-Free Method for Detection of SARS-CoV-2 in Salivary Samples for Mass Screening for COVID-19

Evaluation of RNA extraction free method for detection of SARS-COV-2 in salivary samples for mass screening for COVID-19

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