Inflammatory pre-conditioning of mesenchymal multipotent stromal cells improves their immunomodulatory potency in acute pyelonephritis in rats

Plotnikov, Egor Y.; Pulkova, N. V.; Pevzner, Irina B.; Zorova, Ljubava D.; Silachev, D. N.; Morosanova, Maria A.; Сухих, Г. Т.; Zorov, Dmitry B.

doi:10.1016/j.jcyt.2013.02.003

Cited by 14 publications

(11 citation statements)

References 46 publications

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“…102 Furthermore, in in vivo study, pretreating MSCs with TNF- α /or a cocktail of cytokines resulted in enhanced MSC engraftment efficiency and improvement in acute pyelonephritis. 103 Thus, many factors can influence MSC mobilization and engraftment. Therefore, it is critical to accurately assess and tightly control the properties of cultured MSCs for clinical application; otherwise, the physiological microenvironments they encounter may cause them to behave in unexpected ways.…”

Section: Engraftment Of Mscs At Sites Of Injury or Inflammationmentioning

confidence: 99%

Immunobiology of mesenchymal stem cells

et al. 2013

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Mesenchymal stem cells (MSCs) can be isolated from almost all tissues and effectively expanded in vitro. Although their true in situ properties and biological functions remain to be elucidated, these in vitro expanded cells have been shown to possess potential to differentiate into specific cell lineages. It is speculated that MSCs in situ have important roles in tissue cellular homeostasis by replacing dead or dysfunctional cells. Recent studies have demonstrated that in vitro expanded MSCs of various origins have great capacity to modulate immune responses and change the progression of different inflammatory diseases. As tissue injuries are often accompanied by inflammation, inflammatory factors may provide cues to mobilize MSCs to tissue sites with damage. Before carrying out tissue repair functions, MSCs first prepare the microenvironment by modulating inflammatory processes and releasing various growth factors in response to the inflammation status. In this review, we focus on the crosstalk between MSCs and immune responses and their potential clinical applications, especially in inflammatory diseases.

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Section: Engraftment Of Mscs At Sites Of Injury or Inflammationmentioning

confidence: 99%

Immunobiology of mesenchymal stem cells

et al. 2013

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“…Plotnikov et al (10) report that human MSCs decreased inflammation and oxidative stress in a rat pyelonephritis model of kidney inflammation. MSC treatment was associated with reduced kidney tissue tumor necrosis factor α, reactive oxygen species (ROS) production, malonyldialdehyde, nitrite/nitrate ratio and myeloperoxidase activity.…”

mentioning

confidence: 99%

Multipotent mesenchymal stromal cells protect against kidney injury

Tögel

Bonventre²

2013

Cytotherapy

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“…The section of the day 16 panel was taken at 20x magnification. Control picture was taken on day 8 in serum-containing culture medium neurotrophic factors within the microenvironment (Plotnikov et al 2013). These factors could serve as internal signaling molecules to guide SHED to differentiate into dopaminergic neurons accordingly.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported the success of this technique in various aspects such as bone regeneration (Lu et al 2013), immunomodulatory potency (Plotnikov et al 2013), renal ischemic injury amelioration (Masoud et al 2012), infarcted myocardium restoration (Choudhery et al 2012) and many more. On top of this, choosing proper cues is another vital factor to consider in order to maximize the pre-conditioning effect.…”

Section: Introductionmentioning

confidence: 99%

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Gnanasegaran

Govindasamy²,

Musa

et al. 2014

Cytotechnology

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Among the debilitating diseases, neurological related diseases are the most challenging ones to be treated using cell replacement therapies. Recently, dental pulp stem cells (SHED) were found to be most suitable cell choice for neurological related diseases as evidenced with many preclinical studies. To enhance the neurological potential of SHED, we recapitulated one of the pharmacological therapeutic tools in cell replacement treatment, we pre-conditioned dental pulp stem cells (SHED) with culture medium of ReNCell VM, an immortalized neuron progenitor cell, prior to neurogenesis induction and investigated whether this practice enhances their neurogenesis potential especially towards dopaminergic neurons. We hypothesed that the integration of pharmacological practices such as co-administration of various drugs, a wide range of doses and duration as well as pre-conditioning into cell replacement may enhance the efficacy of stem cell therapy. In particular, pre-conditioning is shown to be involved in the protective effect from some membrano-tropic drugs, thereby improving the resistance of cell structures and homing capabilities. We found that cells pre-treated with ReNCell VM conditioned medium displayed bipolar structures with extensive branches resembling putative dopaminergic neurons as compared to nontreated cells. Furthermore, many neuronal related markers such as NES, NR4A2, MSI1, and TH were highly expressed (fold changes [ 2; p \ 0.05) in pretreated cells. Similar observations were detected at the protein level. The results demonstrate for the first time that SHED pre-conditioning enhances neurological potential and we suggest that cells should be primed to their respective environment prior to transplantation.

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Inflammatory pre-conditioning of mesenchymal multipotent stromal cells improves their immunomodulatory potency in acute pyelonephritis in rats

Cited by 14 publications

References 46 publications

Immunobiology of mesenchymal stem cells

Immunobiology of mesenchymal stem cells

Multipotent mesenchymal stromal cells protect against kidney injury

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

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