Inflammatory profile of vertically HIV-1 infected adolescents receiving ART in Cameroon: a contribution toward optimal pediatric HIV control strategies

Ka’e, Aude Christelle; Nanfack, Aubin Joseph; Ambada, Georgia; Santoro, Maria Mercedes; Takou, Desire; Semengue, Ezechiel Ngoufack Jagni; Nka, Alex Durand; Bala, Marie Laure Mpouel; Endougou, Orphelie Ndoh; Elong, Elise; Beloumou, Grace; Djupsa, Sandrine; Gouissi, Davy Hyacinthe; Fainguem, Nadine; Tchouaket, Michel Carlos Tommo; Sosso, Samuel Martin; Kesseng, Daniel; Ndongo, Francis Ateba; Sonela, Nelson; Kamta, Arnaud Cedric Lacmago; Tchidjou, Hyppolite K.; Ndomgue, Therese; Ndiang, Suzie Tetang Moyo; Nlend, Anne Esther Njom; Nkenfou, Celine Nguefeu; Montesano, Carla; Halle-Ekane, Gregory Edie; Cappelli, Giulia; Tiemessen, Caroline T.; Colizzi, Vittorio; Ceccherini-Silberstein, Francesca; Perno, Carlo-Federico; Fokam, Joseph

doi:10.3389/fimmu.2023.1239877

Cited by 3 publications

(1 citation statement)

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“…The concentrations were calculated using standard curves. Cytokine/chemokine levels were measured in pg/mL, according to previous protocols [ 38 , 39 ].…”

Section: Methodsmentioning

confidence: 99%

Immunity in the Progeroid Model of Cockayne Syndrome: Biomarkers of Pathological Aging

Zayoud,

Chikhaoui,

Kraoua

et al. 2024

Cells

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Cockayne syndrome (CS) is a rare autosomal recessive disorder that affects the DNA repair process. It is a progeroid syndrome predisposing patients to accelerated aging and to increased susceptibility to respiratory infections. Here, we studied the immune status of CS patients to determine potential biomarkers associated with pathological aging. CS patients, as well as elderly and young, healthy donors, were enrolled in this study. Complete blood counts for patients and donors were assessed, immune cell subsets were analyzed using flow cytometry, and candidate cytokines were analyzed via multi-analyte ELISArray kits. In CS patients, we noticed a high percentage of lymphocytes, an increased rate of intermediate and non-classical monocytes, and a high level of pro-inflammatory cytokine IL-8. In addition, we identified an increased rate of particular subtypes of T Lymphocyte CD8+ CD28− CD27−, which are senescent T cells. Thus, an inflammatory state was found in CS patients that is similar to that observed in the elderly donors and is associated with an immunosenescence status in both groups. This could explain the CS patients’ increased susceptibility to infections, which is partly due to an aging-associated inflammation process.

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“…The concentrations were calculated using standard curves. Cytokine/chemokine levels were measured in pg/mL, according to previous protocols [ 38 , 39 ].…”

Section: Methodsmentioning

confidence: 99%

Immunity in the Progeroid Model of Cockayne Syndrome: Biomarkers of Pathological Aging

Zayoud,

Chikhaoui,

Kraoua

et al. 2024

Cells

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Potential antiviral and immunomodulatory Amazonian medicinal plant compounds

Napoleão,

Bernardes,

Tenório

et al. 2024

Preprint

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Background: Novel antiretroviral drugs are in constant need for HIV/AIDS patients to face the continuous emerging resistance to the commonly prescribed combination of anti-HIV synthetic agents and their side effects. Methods: Amazonian medicinal plants, Licania macrophylla (Chrysobalanaceae) and Ouratea hexasperma (Ochnaceae) were assayed for antiretroviral and immunomodulatory activity, utilizing an established human leukocyte cell line and the Simian Immunodeficiency Virus. The IL-4, IL-6, IL-8, IL-10 and IFN-y cytokines were quantified after leukocyte culture stimulation with ethanolic plant extracts and challenged with lentivirus infection. Results: Mitotic activity induced by O. hexasperma extract was significatively more pronounced than L. macrophylla extract. Cytokine modulation was recorded in SIV infected cells under independent treatment with O. hexasperma and L. macrophylla extracts. Betulinic acid, ninuriflavone, (-)epigallocathechine, (-)gallocatechine and 4-O-methyl-epigallocatechin were isolated from L. macrophyla. Conclusions: Cellular proliferative activity and cytokine modulation by the extracts assayed have potential applications in the therapy of HIV/AIDS pathology, as the isolated compounds of these plants have been reported for antiviral activity.

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