Inflow water is a major source of trout farming contamination with Salmonella and multidrug resistant bacteria

Antunes, Patrı́cia; Campos, Joana; Mourão, Joana; Pereira, Joana; Novais, Carla; Peixe, Luı́sa

doi:10.1016/j.scitotenv.2018.06.143

Cited by 28 publications

(16 citation statements)

References 38 publications

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“…Antunes et al [70] detected plasmid-mediated quinolone resistance (PMQR) genes in 21% of the water, sediment, and food samples taken at a trout farm, and in 12% of commercialized trout in three supermarkets. These PMQR (plasmid-mediated quinolone resistance) genes ( qnrS1-S2-S3 , qnrB7-B19 , qnrD1 , and oqxAB ) were detected in A. hydrophila , and these are frequently identified in fish farming studies [71,72,73].…”

Section: Horizontal Transfer Mechanismsmentioning

confidence: 99%

Horizontal Gene Transfer and Its Association with Antibiotic Resistance in the Genus Aeromonas spp.

et al. 2019

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The evolution of multidrug resistant bacteria to the most diverse antimicrobials known so far pose a serious problem to global public health. Currently, microorganisms that develop resistant phenotypes to multiple drugs are associated with high morbidity and mortality. This resistance is encoded by a group of genes termed ‘bacterial resistome’, divided in intrinsic and extrinsic resistome. The first one refers to the resistance displayed on an organism without previous exposure to an antibiotic not involving horizontal genetic transfer, and it can be acquired via mutations. The latter, on the contrary, is acquired exclusively via horizontal genetic transfer involving mobile genetic elements that constitute the ‘bacterial mobilome’. This transfer is mediated by three different mechanisms: transduction, transformation, and conjugation. Recently, a problem of public health due to implications in the emergence of multi-drug resistance in Aeromonas spp. strains in water environments has been described. This is derived from the genetic material transfer via conjugation events. This is important, since bacteria that have acquired antibiotic resistance in natural environments can cause infections derived from their ingestion or direct contact with open wounds or mucosal tissue, which in turn, by their resistant nature, makes their eradication complex. Implications of the emergence of resistance in Aeromonas spp. by horizontal gene transfer on public health are discussed.

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Section: Horizontal Transfer Mechanismsmentioning

confidence: 99%

Horizontal Gene Transfer and Its Association with Antibiotic Resistance in the Genus Aeromonas spp.

et al. 2019

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“…We analysed 880 Enterobacteriaceae isolates resistant to third-generation cephalosporins recovered from different non-clinical samples (n = 628): (i) healthy human faeces; (ii) healthy livestock animal faeces (78 swine, 44 chickens), (iii) uncooked chicken carcasses for human consumption, (iv) water/wastewater samples of aquatic environments, (v) and water/sediment/feed samples of trout aquaculture environments ( Figure 1). Samples were collected from different regions of Portugal, and processed as described [4][5][6][7]. Briefly, the aliquots of uncooked chicken carcasses (0.2 mL) and aquaculture samples (0.1 mL) pre-enriched in buffered peptone water, and aliquots of the remaining samples (0.2 mL of human or animal faeces, 0.1 mL of wastewaters, filters from filtered water of aquatic environments) were seeded on CHROMagarTM Orientation/MacConkey agar plates supplemented with ceftazidime (1 mg/L) or cefotaxime (1 mg/L) [4][5][6][7].…”

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confidence: 99%

“…Samples were collected from different regions of Portugal, and processed as described [4][5][6][7]. Briefly, the aliquots of uncooked chicken carcasses (0.2 mL) and aquaculture samples (0.1 mL) pre-enriched in buffered peptone water, and aliquots of the remaining samples (0.2 mL of human or animal faeces, 0.1 mL of wastewaters, filters from filtered water of aquatic environments) were seeded on CHROMagarTM Orientation/MacConkey agar plates supplemented with ceftazidime (1 mg/L) or cefotaxime (1 mg/L) [4][5][6][7]. Representative Enterobacteriaceae isolates (oxidase negative, approximately one to five unique morphotypes per plate) were recovered from the agar plates supplemented with antibiotics.…”

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confidence: 99%

Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

et al. 2020

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We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing Enterobacteriaceae from different non-clinical environments in Portugal. We analysed 880 Enterobacteriaceae resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-CeuI-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among Enterobacteriaceae from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing Escherichia coli from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013-2014 versus 0% in [2001][2002][2003][2004], which is in accordance with the trend observed in other European countries. CMY-2-producing E. coli belonged to B2 2 -ST4953 (n = 2, HH), A 0 -ST665 (n = 1, HH) or A 1 -ST48 (n = 1, UCC) clones. bla CMY-2 was identified in non-typeable and IncA/C 2 plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing Enterobacteriaceae occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of bla qAmpC in non-clinical settings.

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“…The present study confirmed that these genes persist ubiquitously in aquatic environments, which is consistent with the results of previous reports. Similarly, river water inflowing to trout farms has been reported to be a source of contamination by human-derived Salmonella ( Antunes et al, 2018 ). Seawater also has been reported to serve as a reservoir of Escherichia coli with ARGs that are carried by seagulls ( Alves et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Tetracycline Resistance Gene Profiles in Red Seabream (Pagrus major) Intestine and Rearing Water After Oxytetracycline Administration

et al. 2020

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Marine aquaculture fish and the environment are possible hot spots for the maintenance and spread of antibiotic resistance genes (ARGs). We here show the time courses of changes of six tetracycline resistance genes (tet) in fish rearing seawater and fish intestine in tank experiments. Experimental tanks were prepared as oxytetracycline (OTC) administration tanks and those without OTC. It was found that tet(B), tet(M), and tet(W) were dominant in seawater among the six tet genes. tet(B) and tet(M) abundances increased immediately after OTC administration, indicating that OTC served as a selective pressure to increase the proportion of tet-possessing bacteria. In contrast, the abundance of tet genes in the fish intestine did not differ between the with-and without-OTC administration groups, and clearly was not altered by OTC administration. Profile changing of tet in seawater and fish intestine did not synchronize. These observations suggested that the dynamics of intestinal tet-possessing bacteria do not directly reflect the environment, but reflect selection within the intestine.

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Inflow water is a major source of trout farming contamination with Salmonella and multidrug resistant bacteria

Cited by 28 publications

References 38 publications

Horizontal Gene Transfer and Its Association with Antibiotic Resistance in the Genus Aeromonas spp.

Horizontal Gene Transfer and Its Association with Antibiotic Resistance in the Genus Aeromonas spp.

Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

Tetracycline Resistance Gene Profiles in Red Seabream (Pagrus major) Intestine and Rearing Water After Oxytetracycline Administration

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