Influence des acides linoléique (18:2 n-6) et α-linolénique (18:3 n-3) sur la composition, la perméabilité et la fluidité des phospholipides cardiaques du rat : étude à l'aide de modèles membranaires (liposomes)

Rocquelin, G.; YOYO, N.; Ducruet, Jean‐Marc; Guenot, L.

doi:10.1051/rnd:19860109

Cited by 12 publications

(2 citation statements)

References 18 publications

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“…The specific role of 18:3 n-3 of LEAR oil in the etiology of cardiac necrosis was postulated by McCutcheon et al (1976) and Trenholm et al (1979) Clandinin, 1980;McMurchie et aL, 1983;Rocquelin et aL, 1986;McMurchie, 1988). Preliminary results in our laboratory (Grynberg et al, 1984) …”

Section: Resultsmentioning

confidence: 99%

Relationship between lipid parameters and the occurrence and severity of lesions in the heart : a study on rats fed low erucic acid rapeseed oil

Rocquelin¹,

Juanéda²,

Astorg³

et al. 1989

Reprod. Nutr. Dévelop.

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Summary -Fifty-six male Wistar SPF rats were fed a diet containing low erucic acid rapeseed (LEAR) oil (15% by weight) as the only source of lipids for 18 wk. Lipid parameters (fatty acid composition and contents of lipid classes) and the occurrence and severity of focal lesions were both determined on the heart of each animal. Four groups were constituted according to the severity of cardiac lesions. Statistical analyses were applied to the data to find a relationship between the lipid parameters and the severity of heart lesions. None of the measured parameters (heart contents of neutral lipids, total phospholipids, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, sphingomyelin and fatty acid composition of each phospholipid class) appeared to be related with the grading of the lesions. Therefore, we failed to find a direct support for the assumption that heart lesions, induced by LEAR oil, are mediated by changes in the lipid and/or fatty acid composition of heart membranes. However, this hypothesis can not be discarded.

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Section: Resultsmentioning

confidence: 99%

Relationship between lipid parameters and the occurrence and severity of lesions in the heart : a study on rats fed low erucic acid rapeseed oil

Rocquelin¹,

Juanéda²,

Astorg³

et al. 1989

Reprod. Nutr. Dévelop.

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“…Le profil en acides gras polyinsaturés des phospholipides cardiaques pourrait avoir une influence considérable sur la régulation de certaines enzymes clés. Cette étude a été réalisée pour savoir si une incorporation sélective d'acides gras n-6 ou n-3 dans les phospholipides membranaires responsible for a decrease in the n-6/n-3 ratio in cardiac phospholipids (Rocquelin et al, 1986;Swanson et al, 1986;Hartog et al, 1987;Nalbone et al, 1988). On the other hand, these changes in cellular lipid environment may induce noticeable alterations in cardiac function (Hock et al, 1987;Grynberg et al, 1988;Lamers et al, 1988) …”

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Influence of dietary linseed oil and sunflower seed oil on some mechanical and metabolic parameters of isolated working rat hearts

Demaison

Grynberg²

1991

Reprod. Nutr. Dévelop.

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Summary ― The role played by membrane lipid environment on cardiac function remains poorly defined. The polyunsaturated fatty acid profile of myocardial phospholipids could be of utmost importance in the regulation of key-enzyme activities. This study was undertaken to determine whether selective incorporation of n-6 or n-3 fatty acids in membrane phospholipids might influence cardiac mechanical performances and metabolism. For 8 wk, male weanling Wistar rats were fed a semipurified diet containing either 10% sunflower seed oil (72% C18:2 n-6) or 10% linseed oil (54% C18:3n-3) as the sole source of lipids. The hearts were then removed and perfused according to working mode with a Krebs-Henseleit buffer containing glucose (11 mM) and insulin (10 UII1). Cardiac rate, coronary and aortic flows and ejection fraction were monitored after 30 min of perfusion. Myocardial metabolism was estimated by evaluating the intracellular fate of 1-!4C palmitate. Sunflower seed oil and linseed oil feeding did not modify either coronary or aortic flow, which suggests that cardiac mechanical work was not affected by the diets. Conversely, cardiac rate was significantly decreased (-18%; P < 0.01) when rats were fed the n-3 polyunsaturated fatty acid rich diet. Radioanalysis of the myocardial metabolism suggested that replacing n-6 polyunsaturated fatty acids by n-3 polyunsaturated fatty acids: i) did not alter palmitate uptake; ii) prolonged palmitate incorporation into cardiac triglycerides; iii) reduced beta-oxidation of palmitic acid. These results support the assumption that dietary fatty acids, particularly n-6 and n-3 fatty acids, play an important role in the regulation of cardiac mechanical and metabolic activity.dietary polyunsaturated fatty acid / membrane phospholipid I isolated perfused rat heart I mechanical activity / metabolism

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Rat vitamin E status and heart lipid peroxidation: Effect of dietary α‐Linolenic acid and marine n−3 fatty acids

Javouhey-Donzel

Guenot

Maupoil³

et al. 1993

Lipids

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Three groups of sixteen male rats each were fed semi-purified diets containing 15% by weight of lipid for a period of 4 wk. The diets contained the same amount of polyunsaturated fatty acids (PUFA) (20% of total fatty acids) and saturated fatty acids (19% of total fatty acids). Dietary PUFA were represented exclusively by linoleic acid (18:2 diet), or 10% linoleic acid and 10% linolenic acid (18:3 diet), or 10% linoleic acid and 10% long-chain n-3 fatty acids (LCn-3 diet). The overall amount of vitamin E was similar in the three diets, i.e., 140, 133 and 129 mg/kg diet, respectively. Following appropriate extraction, tocopherol levels in heart, liver, brain, adipose tissue (AT) and plasma were measured by high-performance liquid chromatography. The level of vitamin E in the heart decreased with n-3 PUFA diets, most markedly with LCn-3 PUFA. Liver and AT vitamin E contents also decreased with n-3 PUFA diets when expressed as micrograms/mg total lipids and micrograms/mg phospholipids, respectively. Total plasma vitamin E was lower in rats fed the LCn-3 diet, but there was no significant difference when expressed as microgram/mg total lipids. Brain vitamin E was not affected by the various diets. In vitro cardiac lipid peroxidation was quantified by the thiobarbituric acid reactive substances (TBARS) test. Heart homogenates were incubated at 37 degrees C for 15 and 30 min in both the absence (uninduced) or presence (induced) of a free radical generating system (1 mM xanthine, 0.1 IU per mL xanthine oxidase, 0.2 mM/0.4 mM Fe/ethylenediaminetetraacetic acid). TBARS release was time-independent but significantly higher when LCn-3 fatty acids were fed to rats in either the uninduced or induced system. The study demonstrated that n-3 PUFA diets can influence vitamin E status of rats even in short-term experiments and can change the susceptibility of the heart to in vitro lipid peroxidation.

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Influence des acides linoléique (18:2 n-6) et α-linolénique (18:3 n-3) sur la composition, la perméabilité et la fluidité des phospholipides cardiaques du rat : étude à l'aide de modèles membranaires (liposomes)

Cited by 12 publications

References 18 publications

Relationship between lipid parameters and the occurrence and severity of lesions in the heart : a study on rats fed low erucic acid rapeseed oil

Relationship between lipid parameters and the occurrence and severity of lesions in the heart : a study on rats fed low erucic acid rapeseed oil

Influence of dietary linseed oil and sunflower seed oil on some mechanical and metabolic parameters of isolated working rat hearts

Rat vitamin E status and heart lipid peroxidation: Effect of dietary α‐Linolenic acid and marine n−3 fatty acids

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