Influence of 2,4-D Growth Regulator and Foliar Explant Source on Coffea arabica Embryogenic Calli

Livramento, Kalynka Gabriella do; Torres, Luana Ferreira; Barbosa, Flávia C. Rufini; Andrade, Jéssica de Castro e; Livramento, Dárlan Einstein do; Paiva, Luciano V.

doi:10.9734/jeai/2018/39558

Cited by 1 publication

(2 citation statements)

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“…The research conducted by Livramento et al (2018) has found that Arabica coffee explants with a lower concentration of 2,4-D auxin exhibit the highest potential for inducing embryogenic callus, in contrast with the findings presented in Table 3. Different effectivity of these plant growth regulators may vary depending on the types of plants and the mother plants used as explant sources.…”

Section: Callus Texturementioning

confidence: 70%

“…In previous studies, callus color was used to distinguish between embryogenic and non-embryogenic callus based on their regeneration ability. Embryogenic callus typically appears as yellowish-white, yellow, or brownish-yellow, while brown callus is an indication of non-embryogenic callus that has lost its cell capability to proliferate and regenerate (Ibrahim & Hartati, 2017;Livramento et al, 2018;Ibrahim et al, 2019;Rismayanti & Nafi'ah, 2021).…”

Section: Callus Colormentioning

confidence: 99%

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Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

Fahira,

Latunra,

Johannes

2023

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Robusta coffee (Coffea canephora) is Indonesia's essential leading trade commodity. Robusta coffee is preferred for its bitter taste. However, robusta coffee productivity decreased due to bad agriculture practice. Tissue culture is an alternative to cultivation with various advantages. The plant growth regulators (PGRs) influence tissue culture’s success. Due to its stability, 2,4 dichlorophenoxyacetic acid has become a common synthetic PGRs. PGRs can be obtained from natural ingredients, such as tomatoes. This study aimed to analyze the effect of 2,4-dichlorophenoxyacetic acid and tomato extract addition and its appropriate concentration for callus induction of robusta coffee in vitro. This research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study used a Complete Randomized Design with two factors. The first factor was the concentration of tomato extract (0%; 7.5%; 10%; and 12.5%). Meanwhile, the second factor was the concentration of 2,4 dichlorophenoxyacetic acid (0 ppm; 1 ppm; 2 ppm; and 3 ppm). Observation parameters include the percentage of callus formation, callus growing time, callus fresh weight, callus color, and callus texture. The quantitative data were analyzed by the Kruskal-Wallis test followed by the Mann-Whitney Test to compare the effect of each treatment. The results showed that among other treatment combinations, treatment with the addition of 2 ppm 2,4-D and 10% tomato extract (T2D2) and treatment with the addition of 3 ppm 2,4-D and 10% tomato extract (T2D3) had the best effect in inducing the callus of robusta coffee.

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Section: Callus Texturementioning

confidence: 70%