Influence of 24- epibrassinolide on in vitro shootlets regeneration via direct organogenesis of Phaseolus vulgaris L.

Abstract

This study aimed to optimize an in vitro shootlets regeneration system via direct organogenesis for Phaseolus vulgaris L. cv. Brunca using 24-epibrassinolide (24-Epi). The best medium for shootlets proliferation as well as growth parameters was MS supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi. Stem explants recorded the best results of shootlets proliferation percentage compared with leaf or root explants, respectively. MS medium supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi gave the best results of recovered shootlets (%); photosynthetic pigments, number of leaves, number of shootlets and shootlet length (cm). From the obtained results it can be recognized that, the behavior and trends of 24-Epi on in vitro P. vulgaris cultures media may be oriented and arranged to the behavior of auxins in the nutrient medium for roots formation and as a cytokinin in shootlets proliferation.

Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus ( Phoenix dactylifera L.) using protein profile and RAPD markers

“…Apparently protein electrophoresis is an effective procedure for determination of genetic stability among different treatments, several studies revealed the reliability of this technique in identification of the genetic variations in date palm and many other plants [20], [23], [61], [34], [22], [48]. Genetic impact of different chemicals has been identified using protein SDS-PAGE electrophoresis including PGRs; hydrogen peroxide and sodium salts [38], [35], [29], [4], [51], [52].…”