Influence of 670 nm low-level laser therapy on mast cells and vascular response of cutaneous injuries

Pereira, Manoela Carrera Martinez Cavalcante; Pinho, Cristina Bacellar de; Medrado, Alena Ribeiro Alves Peixoto; Andrade, Zilton de Araújo; Reis, Sílvia Regina de Almeida

doi:10.1016/j.jphotobiol.2009.12.005

Cited by 69 publications

(56 citation statements)

References 27 publications

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“…Besides, elevated numbers of polymorphonuclear cells within the first experimental hours, followed by decreased levels on day 3 post-treatment in laser treated wounds have been reported in previous studies, and a close relation between this phenomenon and the stimulation of mast cells degranulation has been pointed 19 . Therefore, LLLT might provide a short term acute inflammatory response in earlier stages of wound healing, accelerating the phagocutic inflammatory phase of the cicatricial repair.…”

Section: Discussionsupporting

confidence: 67%

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Effect of low level laser on sutured wound healing in rats

et al. 2011

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Purpose:To evaluate the effect of low-level laser therapy (LLLT) ë904 nm on healing of surgical wounds in rats. Methods: Forty male Wistar rats were used, divided into four groups, underwent incision along the lines Alba covering skin, subcutaneous and muscle abdominal, sutured continuously for nylon 5-0. Eight and fifteen days after the surgery process, the repairing area was removed and histological sections were stained with hematoxylin-eosin to assess cellularity inflammatory, Masson´s Trichrome and Picrossirus to quantify the collagen fibers and immunohistochemical technique for counting newly formed vessels. The data were compared statistically using analysis of variance ANOVA, with a "post-hoc Tukey test, p <0.05. Results: Low-level laser therapy reduced the intensity of the inflammatory reaction and influenced the dynamic of the immunoinflammatory response by inducing switching of the leukocyte infiltration pattern (neutrophilic to lymphoplasmacytic infiltration). Also stimulate the deposition and enhance the organization of collagen fibers, featuring a delicate collagen type III. Furthermore, it appeared to a significant increase in the average number of newly formed vessels (p = 0.00 and p = 0.02, respectively). Conclusion: Low-level laser therapy resulted in modulate of the inflammatory response, enhanced deposition of collagen fibers and increase in the average number of newly formed vessels. Key words: Wound Healing. Lasers, Semiconductor. Collagen. Macrophages. Rats. RESUMO Objetivo:Avaliar o efeito da terapia laser de baixa potência (TLBP) ë904 nm na cicatrização de feridas cirúrgicas em ratos. Métodos: Foram utilizados 40 ratos Wistar, machos, divididos em quatro grupos, submetidos à incisão na linha Alba abrangendo pele, subcutâneo e musculatura abdominal, suturados continuamente por fios de nylon 5-0. Oito e quinze dias após o procedimento cirúrgico, a área de reparo foi removida e os cortes histológicos foram corados com hematoxilina-eosina para avaliar celularidade do infiltrado inflamatório, Tricrômico de Masson e Picrosirius para quantificar as fibras de colágeno e técnica imuno-histoquímica para a contagem de vasos neoformados. Os dados foram comparados estatisticamente pela análise de variância ANOVA, com "teste de Tukey post-hoc, p <0,05. Resultados: A terapia laser de baixa potência reduziu a intensidade da reação inflamatória e influenciou a dinâmica da resposta imuno por induzir a mudança do padrão de infiltração de leucócitos (neutrófilos a infiltração mononuclear). Como também estimula a deposição e melhora padrão organizacional das fibras colágenas, apresentando um delicado colágeno tipo III. Além disso, verificou-se um aumento significativo no número médio de vasos neoformados (p = 0,00 e p = 0,02, respectivamente). Conclusão: A terapia laser de baixa potência modula a resposta inflamatória, com melhor deposição de fibras colágenas e aumento do número médio de vasos neoformados. Descritores: Cicatrização de Feridas. Lasers Semicondutores. Colágeno. Macrófagos. Ratos. -Act...

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Section: Discussionsupporting

confidence: 67%

“…On the other hand, in the later stages of the repair process the fibers exhibit an organizational pattern more compact, thicker bundles of red-orange color, indicating a type I collagen 2,11,19 .…”

Section: Discussionmentioning

confidence: 99%

Effect of low level laser on sutured wound healing in rats

et al. 2011

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“…The induction of skin lesions using a 6-mm skin punch was performed successfully in all animals and helped standardize lesions of similar sizes in the experimental animals, similarly to techniques reported in previous studies (KAWALEC et al, 2004;PEREIRA et al, 2010;FIORIO et al, 2017).…”

Section: Discussionmentioning

confidence: 85%

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

Carvalho

Bizo

Toma

et al. 2017

AVB

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Article historyThe use of photobiomodulation therapy (PBMT) has shown promising results for the inflammatory modulation of tissue repair. This study aimed to evaluate the antiinflammatory potential of gallium arsenide (GaAs) PBMT in skin wound healing via (1) measurement of the temperature on the surface of the skin wound; (2) white blood cell count; and (3) histopathological examination. Skin lesions were induced on the dorsum of 20 Wistar rats, after which the animals were divided into two groups: a treatment group, subjected to GaAs PBMT, and a control group. Thermography was performed daily in all of the study animals until the end of the experiment. On the fourth day after lesion induction, whole blood was collected (white blood cell count), animals were euthanized, and skin lesions were biopsied (histopathological examination). There were no differences in the number of leukocytes and in the histopathological evaluations between the groups; however, the thermography analysis indicated an increase in temperature in the treated group. The antiinflammatory activity of GaAs PBMT was not confirmed. The increase in temperature on the surface of the skin lesions after LLLT requires further elucidation because this result could not be justified by the direct action of PBMT.

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“…Concurrently, Shuvaeva, Gorshkova, Kostylev, & Dvoretsky (2011) irradiated normotensive or hypertensive rats with 473 and 650 nm and observed that, especially for the red wavelength, the laser weakened arterial tone, thereby increasing the constrictor effect of norepinephrine. Pereira, Pinho, Medrado, Andrade, & Reis (2010) irradiated mice with 670 nm, after performing skin lesions, and verified an increased expression of vascular endothelial growth factor (VEGF), increased acute inflammation and vasodilation. Such effect of laser therapy is based on the premise of Aras et al (2010), which may have been observed with regard to the time of awakening of the animal, whereas for the irradiated group the time was significantly lower than that to the control group.…”

Section: Evaluation Of Nociceptionmentioning

confidence: 79%

<b>Interaction between low level laser therapy and anesthesia in wistar rats

Rocha

Silva

Meireles

et al. 2016

Acta Sci. Health Sci.

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ABSTRACT. The aim was to assess whether the low level laser therapy (LLLT), 660 nm, can lower the effect of injectable anesthetics in rats. Wistar rats (n = 20) were used in two steps: 1) grip strength test and measuring the anesthesia time for control (G1A) and irradiated (G1AL) groups; 2) after 15 days, rats received new anesthesia injection and were evaluated for nociception (G2A -control; G2AL -laser). Anesthesia was induced by ketamine hydrochloride (75 mg kg ), by intraperitoneal injection, according to the body weight. LLLT used was 660 nm at four sites along the right hind limb. Anesthesia time was shorter for the G1AL (p = 0.0031). There were significant differences between pre-and post-intervention in the grip strength test (p < 0.001), but no differences were detected between groups (p = 0.459). For nociception, G2AL achieved higher values than G2A (p = 0.019 and p = 0.032), and also for the comparison between pre-injury values with the following values (p < 0.001) although no significant difference was found between 10 and 60 minutes (p > 0.05). It can be concluded that the LLLT caused no significant reduction on the effect of anesthesia.Keywords: low-level laser therapy. anesthesia. pain measurement.Interações entre laser de baixa potência e anestesia em ratos wistar RESUMO. O objetivo deste estudo é avaliar se o laser de baixa potência (LBP) pode desempenhar algum efeito na diminuição da ação de anestésicos injetáveis. Foram utilizados 20 ratos Wistar, em duas etapas: 1) realizado teste de força de preensão e contagem de tempo de anestesia para animais controle (G1A) e irradiados (G1AL); 2) após 15 dias, novo procedimento anestésico e avaliada a nocicepção (G2A -controle; G2AL -laser). Foram utilizados os anestésicos Quetamina (75 mg kg -1 ) e Xilazina (10 mg kg -1 ), de acordo com o peso corporal, com injeção intraperitoneal. O laser utilizado foi 660 nm, em 4 locais, ao longo do membro pélvico direito. O tempo cronometrado de anestesia foi menor para G1AL (p = 0,0031). Na avaliação da força de preensão, houve diferenças significativas (p < 0,001) entre o momento pré x pós, mas não entre os grupos (p = 0,459). Para a nocicepção, G2AL obteve valores maiores do que G2A (p = 0,019 e p = 0,032), bem como ao comparar os valores pré-lesão com os seguintes (p < 0,001), mas sem diferenças entre 10 e 60 minutos (p > 0,05). Conclui-se que o LBP não apresentou grandes interações com relação à diminuição do efeito da anestesia.Palavras-chave: terapia a laser de baixa intensidade. anestesia. medição da dor.

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Influence of 670 nm low-level laser therapy on mast cells and vascular response of cutaneous injuries

Cited by 69 publications

References 27 publications

Effect of low level laser on sutured wound healing in rats

Effect of low level laser on sutured wound healing in rats

Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

<b>Interaction between low level laser therapy and anesthesia in wistar rats

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