Influence of a 12‐hour, 22°C holding period for buffy coats on the preparation of platelet concentrates stored in plasma

Abstract

BCs can be held at 22 degrees C for up to 12 hours, with no detrimental effect on the quality of PCs stored for up to 5 days in plasma. Such a holding time might help overcome logistic problems in blood banks.

Effect of holding buffy coats 4 or 18 hours before preparing pooled filtered PLT concentrates in plasma

“…Perhaps this increase in the expression of apoptotic makers was responsible for the progressive decline in PLT concentration observed over storage. This decline has also been reported by other authors, although the decrease was limited to 7.8 percent after 8 days of storage 12 …”

“…The effect of holding BCs before preparing nonleukoreduced PCs has previously been investigated by several authors 12 . ‐15 Boeri and coworkers 12 reported in nonleukoreduced single PCs in plasma prepared after holding BCs for 3 or 12 hours similar PLT yield, pH, hypotonic shock response, morphologic scores, and dense granule content after 5 days of storage.…”

“…The effect of holding BCs before preparing nonleukoreduced PCs has previously been investigated by several authors 12 . ‐15 Boeri and coworkers 12 reported in nonleukoreduced single PCs in plasma prepared after holding BCs for 3 or 12 hours similar PLT yield, pH, hypotonic shock response, morphologic scores, and dense granule content after 5 days of storage. Ledent and colleagues 13 compared the degree of α granule release (measured as PLT‐derived growth factor loss) in PCs prepared by the PRP method and by the BC removal method in top and bottom bags after storing the BCs 4 and 24 hours.…”

In vitro Properties of Platelet Concentrates

“…Variation of the holding time of BC also affects platelet yields and, additionally, platelet function. Reducing the BC holding time from 3 h to 0-90 min reduces platelet yields [29] and function [5]. Increasing the BC holding time to 16 h, although increasing platelet yields, is disadvantageous because of significant negative effects on pH value, morphology score, lactate, and β-TG [6].…”

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…However, buffy coats can also be held for a couple of hours or, in case of whole blood processing within 2 to 8 hours after collection, even overnight at room temperature for pooling and processing in platelet concentrates the next day. One study evaluated a number of holding times of the buffy coat, prepared after 3-to 6-hour hold of the whole blood, and saw an increase in platelet yields in those buffy coats: 52% ± 7% when buffy coats were immediately processed into platelet concentrates, 53% ± 10% when held for 90 minutes, 73% ± 4% when held for 3 hours, and lastly 74% ± 9% when the buffy coat was held for 12 hours before processing [38]. A more in-depth evaluation of 5-day stored platelet concentrates from the 3-vs 12-hour-held buffy coats showed no difference for pH, HSR, morphology scores, dense granula content, collagen-induced aggregation, and thromboxane production, but, ß-thromboglobulin content and thrombininduced aggregation were somewhat better for platelet concentrates from buffy coats held for 12 hours than for 3 hours.…”