Influence of a Diester Glucocorticoid Spray on the Cortisol Level and the CCR4⁺CD4⁺Lymphocytes in Dogs with Atopic Dermatitis: Open Study

Abstract

This study investigated the influence of 0.00584% hydrocortisone aceponate spray (HCA; Cortavance Virbac SA, Carros, France) on blood serum cortisol levels and peripheral blood CCR4+ CD4+ T-lymphocyte levels in dogs with atopic dermatitis. Patients were randomly divided into group I (N = 8) and group II (N = 8). The dogs in group I were sprayed with HCA on the affected skin once a day for three weeks. The dogs in group II were treated once a day for 3 days followed by no treatment for 4 days for a total of three weeks. For the dogs in group I and group II the CADESI-03 scores before and after use of HCA showed significant reduction (P < 0.01). The postcortisol level after the use of HCA in group I showed 36.0% decrease and showed significant suppression (P < 0.01). By comparison, the use of HCA on group II did not show decrease in postcortisol levels. There was a tendency of suppression for hypothalamus—pituitary gland—adrenal gland system, but it was not serious influence. In addition, there was no influence on peripheral blood CCR4+ CD4+ lymphocytes percentage in dogs in group I after treatment with HCA.

Phenotypic analysis of mice xenografted with canine epitheliotropic cutaneous T‐cell lymphoma cells

“…White blood cells were isolated from peripheral blood using lysing buffer (BD Biosciences; Franklin Lakes, NJ, USA), followed by an incubation with fluorescein isothiocyanate (FITC)‐conjugated mouse anti‐canine CD3 (clone CA17.2A12, Bio‐Rad Laboratories; Hercules, CA, USA), allophycocyanin (APC)‐conjugated rat anti‐canine CD8 (clone YCATE55.9, Bio‐Rad Laboratories) and phycoerythrin (PE)‐conjugated mouse anti‐human CCR4 (clone 1G1, BD Biosciences) monoclonal antibodies in washing buffer at 4°C for 30 min. Antibodies with appropriate isotypes (FITC‐conjugated mouse IgG1, clone MOPC‐21, Bio Legend; APC‐conjugated rat IgG1, clone 43414, R&D Systems; Minneapolis, MN, USA; PE‐conjugated mouse IgG1, clone MOPC‐21, Bio Legend) were used for each sample.…”

Corrigendum to “Influence of a Diester Glucocorticoid Spray on the Cortisol Level and the CCR4+ CD4+ Lymphocytes in Dogs with Atopic Dermatitis: Open Study”

“…Our paper titled “Influence of a Diester Glucocorticoid Spray on the Cortisol Level and the CCR4 + CD4 + Lymphocytes in Dogs with Atopic Dermatitis: Open Study” [ 1 ] contains an error in Abstract and Introduction. The concentration of hydrocortisone aceponate spray was incorrectly reported as 0.00584% while the correct value is 0.0584%.…”