Influence of a PGF2.ALPHA.-Analogue, Etiproston Tromethamine, on the Functional Corpus Luteum of Dogs

Abstract

ABSTRACT. To induce luteal regression-related abortion/delivery and treat pyometra in dogs, various PGF 2α -analogues (PGAs) are administered, but a PGA most appropriate for clinical application in dogs, with a low incidence of side effects, is being investigated. In this study, we compared the effects of etiproston tromethamine (PGA-E), which has not been investigated in dogs, with those of cloprostenol (PGA-C), which is routinely used in dogs. A single dose of PGA-E at 100, 200, 400 or 800 µg or PGA-C at 12.5, 25, 50 or 100 µg was administered to beagles (n=5 per group) 25 days after ovulation, when the corpus luteum was in the functional phase. We compared the state of luteal regression by measuring plasma progesterone levels. As side effects, the incidences of salivation, vomiting, tachypnea, diarrhea and the drop in body temperature were investigated. In the 400-µg and 800-µg groups treated with PGA-E, the mean intervals from administration until luteal regression were 18.6 days and 31.2 days, respectively. In the dogs treated with 50 µg or more of PGA-C, luteal regression was noted 2 days after administration. The above side effects were observed for 3 hr after administration of PGA-E/PGA-C. In the dogs treated with 800 µg of PGA-E, the mean body temperature was 36.7°C 4 hr after administration; hypothermia persisted. PGA-E may be less useful than PGA-C for promoting luteal regression in dogs in clinical application.

Regulation of prostaglandin D synthase and prostacyclin synthase in the endometrium of cyclic, pregnant, and pseudopregnant rats and their regulation by sex steroids

“…However, PGHS-1 and PGHS-2 transcripts are encoded by distinct genes located on different chromosomes and differ in size (Xie et al 1991, Williams & DuBois 1996. PGHS-1 is a constitutive enzyme present in many mammalian cells (Kraemer et al 1992, Kujubu & Herschman 1992, Kennedy 1994 and its expression appears to be regulated developmentally (Kirihara et al 2005). PGHS-2 is undetectable in most mammalian tissues (Kennedy 1994), but its expression can be induced by growth factors, cytokines, and tumor promoters (Simmons et al 1991, Evett et al 1993, O'Neill & Ford-Hutchinson 1993.…”