Influence of a step-change in metal exposure (Cd, Cu, Zn) on metal accumulation and subcellular partitioning in a freshwater bivalve, Pyganodon grandis: A long-term transplantation experiment between lakes with contrasting ambient metal levels

Cooper, Sophie; Bonneris, Emmanuelle; Michaud, Annick L.; Pinel‐Alloul, Bernadette; Campbell, Peter G. C.

doi:10.1016/j.aquatox.2013.01.021

Cited by 22 publications

(14 citation statements)

References 25 publications

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“…Furthermore, for O. mykiss and Chironomus riparius , an important part of lysosomes was also recovered in the cytosol fraction. Therefore, interpretation of results of metal subcellular distribution from studies that considered the separation of lysosomes without prior validation must consider the potentially important overlap between this fraction and the mitochondrial and cytosolic fractions (Giguère et al ; Kamunde ; Cooper et al ). However, many studies did not make the separation between mitochondrial and microsomal fractions, labeling the fraction containing these two components as “organelles” (Wallace and Luoma ; Rainbow et al ; Geffard et al ).…”

Section: Discussionmentioning

confidence: 99%

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Cardon

Caron

Rosabal

et al. 2018

Limnology & Ocean Methods

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The use of fractionation protocols to determine metal subcellular distribution in aquatic organisms has gained much interest over the last 15 yr, however, accurate separations among the different components of cells are challenging. Subcellular fractions separated with such an approach are operationally defined and a potentially significant difference can exist between anticipated and resulting fractions. This study customizes and validates subcellular partitioning protocols, for three different freshwater organisms representing a diversity of challenges for subcellular fractionation: Daphnia magna, Chironomus riparius, and liver of Oncorhynchus mykiss. Several protocols involving different homogenization methods, centrifugation speeds, or conservation conditions were tested, and their efficiencies were assessed using enzymatic biomarker assays. Our work allowed us to identify critical steps to improve separations. First, for D. magna, a crustacean with a reinforced chitinous exoskeleton, the use of a strong homogenization method using a sonicator is necessary. Second, for both invertebrates, we observed the leaking of the mitochondrial matrix during cell fractionation, regardless of the homogenization strength and conservation conditions. Therefore, we propose that the mitochondria fraction should be referred to as the mitochondrial membrane fraction, and the cytosol fraction should be identified as the cytosol and mitochondrial matrix fraction. Third, the presence of a lipid-rich layer during O. mykiss liver fractionation may lead to an overlap between mitochondria and cytosol and must be considered in the protocol development. Finally, lysosomes should not be pooled with the microsomes fraction without prior validation. Overall, this study provides a benchmark for future methodological studies on similar taxa.

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Section: Discussionmentioning

confidence: 99%

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Cardon

Caron

Rosabal

et al. 2018

Limnology & Ocean Methods

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“…Although low Cd levels were recorded in the present study, a BAF higher than 1 was recorded in digestive gland samples (Table ). Previous studies have shown that in mussel whole soft tissues, bioaccumulation of Cd occurs over time after exposure, indicating a very slow or negligible elimination rate of this element . This slow rate can be explained by the fact that, as MT degrades, the Cd released induces synthesis of a new protein, to which the metal becomes resequestered .…”

Section: Discussionmentioning

confidence: 99%

Evaluation and comparison of trace metal accumulation in different tissues of potential bioindicator organisms: Macrobenthic filter feeders Styela plicata, Sabella spallanzanii, and Mytilus galloprovincialis

Bellante

Piazzese

Cataldo

et al. 2016

Enviro Toxic and Chemistry

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Trace metal concentrations were measured in different tissues of Sabella spallanzanii, Styela plicata, and Mytilus galloprovincialis collected in the Termini Imerese Harbor (Sicily, Italy) to evaluate the potential use of these species as bioindicators. Higher bioaccumulation factors (BAFs) were calculated in the tube of S. spallanzanii, except for As, which had a higher BAF in the branchial crown of the same species. Regarding the other species analyzed, higher BAFs were found in the digestive gland of M. galloprovincialis. An exception was Pb, which was significantly more concentrated in the branchial basket and tunic of S. plicata. The BAFs calculated in the present study show that all the species analyzed accumulate a certain amount of metals as a consequence of filter feeding mechanisms, and thus it was possible to assess the suitability of S. plicata, S. spallanzanii, and M. galloprovincialis as indicators of water quality. In particular, the tube of S. spallanzanii is an important compartment in terms of metal retention and is more suitable for the evaluation of contamination from trace elements. Environ Toxicol Chem 2016;35:3062-3070. © 2016 SETAC.

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“…These services are often at risk due to the increasing stress caused by contaminants from activities related to urbanization, industrialization, intensive agriculture in the watershed, massive tourism, and intensive aquaculture (e.g. Amiard et al 2006, Cooper et al 2013. Among numerous contaminants released to the environment, metals and metalloids are a worldwide concern due to their persistence and toxicity (ATSDR 2015).…”

Section: Introductionmentioning

confidence: 99%

Enhanced trace element concentrations in tissues of the clam <em>Ruditapes decussatus</em> transplanted to areas influenced by human activities (Ria Formosa, Portugal)

et al. 2017

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Summary:To examine the extent to which human activities near the Ria Formosa coastal lagoon influence the accumulation of trace elements (TE) in Ruditapes decussatus, individuals were transplanted from a natural bank located in the lower lagoon to three sites located in clam growth grounds under the influence of a small city (Faro), a fish farming centre (Olhão) and a site near the lagoon inlet (Lavajo). Concentrations were determined in substrate of the clam grounds and in the digestive gland, gills, mantle plus siphons, and remaining tissues of clams in four periods of the year. These measurements were accomplished with the monthly survey of the gametogenic stages, condition index, proteins, glycogen, total lipids, pH and osmolarity of hemolymph. Arsenic, Cu, Mn, V, Cr and Pb were preferentially linked to the digestive gland, while Cd was linked to the gills. TE concentrations in the digestive gland and remaining tissues were higher in winter, most likely reflecting additional inputs associated with rain. The lack of disruptions in biological parameters and the prolonged period of spawning and gonad recovery in clams suggest that the current TE availability in the lagoon has a minor influence on the reproductive cycle and hence on clam production. Keywords: trace elements; biological parameters; Ruditapes decussatus; Ria Formosa. Aumento de la concentración de elementos traza en tejidos de la almeja Ruditapes decussatus trasplantada a zonas influenciadas por actividades humanas (ría Formosa, Portugal)Resumen: Para examinar en qué medida las actividades humanas cercanas a la laguna costera ría Formosa influyen en la acumulación de elementos traza (ET) en Ruditapes decussatus, se trasplantaron individuos desde un banco natural situado en la parte baja de la laguna a tres sitios localizados en zonas de cultivo de almejas bajo la influencia de una ciudad pequeña (Faro), un centro piscícola (Olhão) y cerca de la entrada de una laguna (Lavajo). Se determinaron las concentraciones de ET en el substrato de locales y en la glándula digestiva, branquias, manto más sifones, y en los tejidos restantes de las almejas en cuatro períodos a lo largo de un año. Estas mediciones fueron realizadas con el estudio mensual de las etapas gametógenas, índice de condición, proteínas, glicóneo, lípidos totales, pH y osmolaridad de la hemolinfa. El As, Cu, Mn, V, Cr y Pb fueron relacionados preferencialmente a la glándula digestiva, mientras que el Cd fue a las branquias. La concentración de ET en la glándula digestiva y los tejidos restantes fue más elevada en invierno, probablemente reflejando los aportes adicionales asociados con la lluvia. La falta de alteraciones de los parámetros biológicos, el período prolongado de desove y la recuperación de las gónadas en las almejas sugieren que la disponibilidad actual de ET en la laguna tienen una influencia menor durante el ciclo reproductivo y, por lo tanto, en la producción de almejas.

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Influence of a step-change in metal exposure (Cd, Cu, Zn) on metal accumulation and subcellular partitioning in a freshwater bivalve, Pyganodon grandis: A long-term transplantation experiment between lakes with contrasting ambient metal levels

Cited by 22 publications

References 25 publications

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Evaluation and comparison of trace metal accumulation in different tissues of potential bioindicator organisms: Macrobenthic filter feeders Styela plicata, Sabella spallanzanii, and Mytilus galloprovincialis

Enhanced trace element concentrations in tissues of the clam <em>Ruditapes decussatus</em> transplanted to areas influenced by human activities (Ria Formosa, Portugal)

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